1. Measuring the confluence of iPSCs using an automated imaging system
- Author
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Magliocca, V., Vinci, M., Persichini, T., Locatelli, Franco, Tartaglia, M., Compagnucci, C., Locatelli F. (ORCID:0000-0002-7976-3654), Magliocca, V., Vinci, M., Persichini, T., Locatelli, Franco, Tartaglia, M., Compagnucci, C., and Locatelli F. (ORCID:0000-0002-7976-3654)
- Abstract
This study focuses on understanding how growing iPSCs on different ECM coating substrates can affect cell confluence. A protocol to assess iPSC confluence in real time has been established without the need to count cells in single cell suspension to avoid any growth perturbation. A high-content image analysis system was used to assess iPCS confluence on 4 different ECMs over time in an automated manner. Different analysis settings were used to assess cell confluence of adherent iPSCs and only a slight difference (at 24 and 48 hours with laminin) has been observed whether a 60, 80 or 100% mask was applied. We also show that laminin lead to the best confluence compared to Matrigel, vitronectin and fibronectin.
- Published
- 2020