Search

Your search keyword '"Miinalainen, Ilkka"' showing total 11 results
Start Over Author "Miinalainen, Ilkka" Publication Type Electronic Resources
11 results on '"Miinalainen, Ilkka"'

1. Erbb4 regulates the oocyte microenvironment during folliculogenesis

Author

Veikkolainen, Ville, Ali, Nsrein, Doroszko, Milena, Kiviniemi, Antti, Miinalainen, Ilkka, Ohlsson, Claes, Poutanen, Matti, Rahman, Nafis, Elenius, Klaus, Vainio, Seppo J., Naillat, Florence, Veikkolainen, Ville, Ali, Nsrein, Doroszko, Milena, Kiviniemi, Antti, Miinalainen, Ilkka, Ohlsson, Claes, Poutanen, Matti, Rahman, Nafis, Elenius, Klaus, Vainio, Seppo J., and Naillat, Florence

Abstract

Polycystic ovary syndrome (PCOS) is one of the most common endocrine disorders leading to infertility in women affecting reproductive, endocrine and metabolic systems. Recent genomewide association studies on PCOS cohorts revealed a single nucleotide polymorphism (SNP) in the ERBB4 receptor tyrosine kinase 4 gene, but its role in ovary development or during folliculogenesis remains poorly understood. Since no genetic animal models mimicking all PCOS reproductive features are available, we conditionally deleted Erbb4 in murine granulosa cells (GCs) under the control of Amh promoter. While we have demonstrated that Erbb4 deletion displayed aberrant ovarian function by affecting the reproductive function (asynchronous oestrous cycle leading to few ovulations and subfertility) and metabolic function (obesity), their ovaries also present severe structural and functional abnormalities (impaired oocyte development). Hormone analysis revealed an up-regulation of serum luteinizing hormone, hyperandrogenism, increased production of ovarian and circulating anti-Mullerian hormone. Our data implicate that Erbb4 deletion in GCs leads to defective intercellular junctions between the GCs and oocytes, causing changes in the expression of genes regulating the local microenvironment of the follicles. In vitro culture assays reducing the level of Erbb4 via shRNAs confirm that Erbb4 is essential for regulating Amh level. In conclusion, our results indicate a functional role for Erbb4 in the ovary, especially during folliculogenesis and its reduced expression plays an important role in reproductive pathophysiology, such as PCOS development. [GRAPHICS] .

Published
2020
Full Text
View/download PDF

2. The generation and use of recombinant extracellular vesicles as biological reference material

Author

Geeurickx, Edward, Tulkens, Joeri, Dhondt, Bert, Van Deun, Jan, Lippens, Lien, Vergauwen, Glenn, Heyrman, Elisa, De Sutter, Delphine, Gevaert, Kris, Impens, Francis, Miinalainen, Ilkka, Van Bockstal, Pieter-Jan, De Beer, Thomas, Wauben, Marca H M, Nolte-'t-Hoen, Esther N M, Bloch, Katarzyna, Swinnen, Johannes V, van der Pol, Edwin, Nieuwland, Rienk, Braems, Geert, Callewaert, Nico, Mestdagh, Pieter, Vandesompele, Jo, Denys, Hannelore, Eyckerman, Sven, De Wever, Olivier, Hendrix, An, Geeurickx, Edward, Tulkens, Joeri, Dhondt, Bert, Van Deun, Jan, Lippens, Lien, Vergauwen, Glenn, Heyrman, Elisa, De Sutter, Delphine, Gevaert, Kris, Impens, Francis, Miinalainen, Ilkka, Van Bockstal, Pieter-Jan, De Beer, Thomas, Wauben, Marca H M, Nolte-'t-Hoen, Esther N M, Bloch, Katarzyna, Swinnen, Johannes V, van der Pol, Edwin, Nieuwland, Rienk, Braems, Geert, Callewaert, Nico, Mestdagh, Pieter, Vandesompele, Jo, Denys, Hannelore, Eyckerman, Sven, De Wever, Olivier, and Hendrix, An

Abstract

Recent years have seen an increase of extracellular vesicle (EV) research geared towards biological understanding, diagnostics and therapy. However, EV data interpretation remains challenging owing to complexity of biofluids and technical variation introduced during sample preparation and analysis. To understand and mitigate these limitations, we generated trackable recombinant EV (rEV) as a biological reference material. Employing complementary characterization methods, we demonstrate that rEV are stable and bear physical and biochemical traits characteristic of sample EV. Furthermore, rEV can be quantified using fluorescence-, RNA- and protein-based technologies available in routine laboratories. Spiking rEV in biofluids allows recovery efficiencies of commonly implemented EV separation methods to be identified, intra-method and inter-user variability induced by sample handling to be defined, and to normalize and improve sensitivity of EV enumerations. We anticipate that rEV will aid EV-based sample preparation and analysis, data normalization, method development and instrument calibration in various research and biomedical applications

Published
2019

3. The generation and use of recombinant extracellular vesicles as biological reference material

Author

dB&C I&I, LS Celbiologie-Algemeen, Celbiologie, Geeurickx, Edward, Tulkens, Joeri, Dhondt, Bert, Van Deun, Jan, Lippens, Lien, Vergauwen, Glenn, Heyrman, Elisa, De Sutter, Delphine, Gevaert, Kris, Impens, Francis, Miinalainen, Ilkka, Van Bockstal, Pieter-Jan, De Beer, Thomas, Wauben, Marca H M, Nolte-'t-Hoen, Esther N M, Bloch, Katarzyna, Swinnen, Johannes V, van der Pol, Edwin, Nieuwland, Rienk, Braems, Geert, Callewaert, Nico, Mestdagh, Pieter, Vandesompele, Jo, Denys, Hannelore, Eyckerman, Sven, De Wever, Olivier, Hendrix, An, dB&C I&I, LS Celbiologie-Algemeen, Celbiologie, Geeurickx, Edward, Tulkens, Joeri, Dhondt, Bert, Van Deun, Jan, Lippens, Lien, Vergauwen, Glenn, Heyrman, Elisa, De Sutter, Delphine, Gevaert, Kris, Impens, Francis, Miinalainen, Ilkka, Van Bockstal, Pieter-Jan, De Beer, Thomas, Wauben, Marca H M, Nolte-'t-Hoen, Esther N M, Bloch, Katarzyna, Swinnen, Johannes V, van der Pol, Edwin, Nieuwland, Rienk, Braems, Geert, Callewaert, Nico, Mestdagh, Pieter, Vandesompele, Jo, Denys, Hannelore, Eyckerman, Sven, De Wever, Olivier, and Hendrix, An

Published
2019

4. The generation and use of recombinant extracellular vesicles as biological reference material

Author

dB&C I&I, LS Celbiologie-Algemeen, Celbiologie, Geeurickx, Edward, Tulkens, Joeri, Dhondt, Bert, Van Deun, Jan, Lippens, Lien, Vergauwen, Glenn, Heyrman, Elisa, De Sutter, Delphine, Gevaert, Kris, Impens, Francis, Miinalainen, Ilkka, Van Bockstal, Pieter-Jan, De Beer, Thomas, Wauben, Marca H M, Nolte-'t-Hoen, Esther N M, Bloch, Katarzyna, Swinnen, Johannes V, van der Pol, Edwin, Nieuwland, Rienk, Braems, Geert, Callewaert, Nico, Mestdagh, Pieter, Vandesompele, Jo, Denys, Hannelore, Eyckerman, Sven, De Wever, Olivier, Hendrix, An, dB&C I&I, LS Celbiologie-Algemeen, Celbiologie, Geeurickx, Edward, Tulkens, Joeri, Dhondt, Bert, Van Deun, Jan, Lippens, Lien, Vergauwen, Glenn, Heyrman, Elisa, De Sutter, Delphine, Gevaert, Kris, Impens, Francis, Miinalainen, Ilkka, Van Bockstal, Pieter-Jan, De Beer, Thomas, Wauben, Marca H M, Nolte-'t-Hoen, Esther N M, Bloch, Katarzyna, Swinnen, Johannes V, van der Pol, Edwin, Nieuwland, Rienk, Braems, Geert, Callewaert, Nico, Mestdagh, Pieter, Vandesompele, Jo, Denys, Hannelore, Eyckerman, Sven, De Wever, Olivier, and Hendrix, An

Published
2019

5. The generation and use of recombinant extracellular vesicles as biological reference material

Author

dB&C I&I, LS Celbiologie-Algemeen, Celbiologie, Geeurickx, Edward, Tulkens, Joeri, Dhondt, Bert, Van Deun, Jan, Lippens, Lien, Vergauwen, Glenn, Heyrman, Elisa, De Sutter, Delphine, Gevaert, Kris, Impens, Francis, Miinalainen, Ilkka, Van Bockstal, Pieter-Jan, De Beer, Thomas, Wauben, Marca H M, Nolte-'t-Hoen, Esther N M, Bloch, Katarzyna, Swinnen, Johannes V, van der Pol, Edwin, Nieuwland, Rienk, Braems, Geert, Callewaert, Nico, Mestdagh, Pieter, Vandesompele, Jo, Denys, Hannelore, Eyckerman, Sven, De Wever, Olivier, Hendrix, An, dB&C I&I, LS Celbiologie-Algemeen, Celbiologie, Geeurickx, Edward, Tulkens, Joeri, Dhondt, Bert, Van Deun, Jan, Lippens, Lien, Vergauwen, Glenn, Heyrman, Elisa, De Sutter, Delphine, Gevaert, Kris, Impens, Francis, Miinalainen, Ilkka, Van Bockstal, Pieter-Jan, De Beer, Thomas, Wauben, Marca H M, Nolte-'t-Hoen, Esther N M, Bloch, Katarzyna, Swinnen, Johannes V, van der Pol, Edwin, Nieuwland, Rienk, Braems, Geert, Callewaert, Nico, Mestdagh, Pieter, Vandesompele, Jo, Denys, Hannelore, Eyckerman, Sven, De Wever, Olivier, and Hendrix, An

Published
2019

6. Wnt5a Deficiency Leads to Anomalies in Ureteric Tree Development, Tubular Epithelial Cell Organization and Basement Membrane Integrity Pointing to a Role in Kidney Collecting Duct Patterning

Author

Pietilä, Ilkka, Prunskaite-Hyyryläinen, Renata, Kaisto, Susanna, Tika, Elisavet, van Eerde, Albertien M, Salo, Antti M, Garma, Leonardo, Miinalainen, Ilkka, Feitz, Wout F, Bongers, Ernie M H F, Juffer, André, Knoers, Nine V A M, Renkema, Kirsten Y, Myllyharju, Johanna, Vainio, Seppo J, Pietilä, Ilkka, Prunskaite-Hyyryläinen, Renata, Kaisto, Susanna, Tika, Elisavet, van Eerde, Albertien M, Salo, Antti M, Garma, Leonardo, Miinalainen, Ilkka, Feitz, Wout F, Bongers, Ernie M H F, Juffer, André, Knoers, Nine V A M, Renkema, Kirsten Y, Myllyharju, Johanna, and Vainio, Seppo J

Published
2016

7. Wnt5a Deficiency Leads to Anomalies in Ureteric Tree Development, Tubular Epithelial Cell Organization and Basement Membrane Integrity Pointing to a Role in Kidney Collecting Duct Patterning

Author

Pietilä, Ilkka, Prunskaite-Hyyryläinen, Renata, Kaisto, Susanna, Tika, Elisavet, van Eerde, Albertien M, Salo, Antti M, Garma, Leonardo, Miinalainen, Ilkka, Feitz, Wout F, Bongers, Ernie M H F, Juffer, André, Knoers, Nine V A M, Renkema, Kirsten Y, Myllyharju, Johanna, Vainio, Seppo J, Pietilä, Ilkka, Prunskaite-Hyyryläinen, Renata, Kaisto, Susanna, Tika, Elisavet, van Eerde, Albertien M, Salo, Antti M, Garma, Leonardo, Miinalainen, Ilkka, Feitz, Wout F, Bongers, Ernie M H F, Juffer, André, Knoers, Nine V A M, Renkema, Kirsten Y, Myllyharju, Johanna, and Vainio, Seppo J

Published
2016

8. Wnt5a Deficiency Leads to Anomalies in Ureteric Tree Development, Tubular Epithelial Cell Organization and Basement Membrane Integrity Pointing to a Role in Kidney Collecting Duct Patterning

Author

Genetica Klinische Genetica, Child Health, Genetica, Genetica Groep Van Tintelen, Pietilä, Ilkka, Prunskaite-Hyyryläinen, Renata, Kaisto, Susanna, Tika, Elisavet, van Eerde, Albertien M, Salo, Antti M, Garma, Leonardo, Miinalainen, Ilkka, Feitz, Wout F, Bongers, Ernie M H F, Juffer, André, Knoers, Nine V A M, Renkema, Kirsten Y, Myllyharju, Johanna, Vainio, Seppo J, Genetica Klinische Genetica, Child Health, Genetica, Genetica Groep Van Tintelen, Pietilä, Ilkka, Prunskaite-Hyyryläinen, Renata, Kaisto, Susanna, Tika, Elisavet, van Eerde, Albertien M, Salo, Antti M, Garma, Leonardo, Miinalainen, Ilkka, Feitz, Wout F, Bongers, Ernie M H F, Juffer, André, Knoers, Nine V A M, Renkema, Kirsten Y, Myllyharju, Johanna, and Vainio, Seppo J

Published
2016

9. Common and specific effects of TIE2 mutations causing venous malformations.

Author

UCL - (SLuc) Service de chirurgie plastique, UCL - SSS/DDUV - Institut de Duve, Nätynki, Marjut, Kangas, Jaakko, Miinalainen, Ilkka, Sormunen, Raija, Pietilä, Riikka, Soblet, Julie, Boon, Laurence M., Vikkula, Miikka, Limaye, Nisha, Eklund, Lauri, UCL - (SLuc) Service de chirurgie plastique, UCL - SSS/DDUV - Institut de Duve, Nätynki, Marjut, Kangas, Jaakko, Miinalainen, Ilkka, Sormunen, Raija, Pietilä, Riikka, Soblet, Julie, Boon, Laurence M., Vikkula, Miikka, Limaye, Nisha, and Eklund, Lauri

Abstract

Venous malformations (VMs) are localized defects in vascular morphogenesis frequently caused by mutations in the gene for the endothelial tyrosine kinase receptor TIE2. Here, we report the analysis of a comprehensive collection of 22 TIE2 mutations identified in patients with VM, either as single amino acid substitutions or as double-mutations on the same allele. Using endothelial cell (EC) cultures, mouse models and ultrastructural analysis of tissue biopsies from patients, we demonstrate common as well as mutation-specific cellular and molecular features, on the basis of which mutations cluster into categories that correlate with data from genetic studies. Comparisons of double-mutants with their constituent single-mutant forms identified the pathogenic contributions of individual changes, and their compound effects. We find that defective receptor trafficking and subcellular localization of different TIE2 mutant forms occur via a variety of mechanisms, resulting in attenuated response to ligand. We also demonstrate, for the first time, that TIE2 mutations cause chronic activation of the MAPK pathway resulting in loss of normal EC monolayer due to extracellular matrix (ECM) fibronectin deficiency and leading to upregulation of plasminogen/plasmin proteolytic pathway. Corresponding EC and ECM irregularities are observed in affected tissues from mouse models and patients. Importantly, an imbalance between plasminogen activators versus inhibitors would also account for high d-dimer levels, a major feature of unknown cause that distinguishes VMs from other vascular anomalies.

Published
2015

10. Mitogen-activated protein kinase (MAPK) pathway regulates branching by remodeling epithelial cell adhesion

Author

University of Helsinki, Institute of Biotechnology, Iherman-Hella, Anneliis, Lume, Maria, Miinalainen, Ilkka, Pirttiniemi, Anniina, Gui, Yujuan, Peränen, Johan, Charron, Jean, Saarma, Mart, Costantini, Frank, Kuure, Satu Helena, University of Helsinki, Institute of Biotechnology, Iherman-Hella, Anneliis, Lume, Maria, Miinalainen, Ilkka, Pirttiniemi, Anniina, Gui, Yujuan, Peränen, Johan, Charron, Jean, Saarma, Mart, Costantini, Frank, and Kuure, Satu Helena

Published
2014

11. Channel-forming activities in the glycosomal fraction from the bloodstream form of Trypanosoma brucei.

Author

UCL - SSS/DDUV - Institut de Duve, Gualdron-López, Melisa, Vapola, Miia H, Miinalainen, Ilkka J, Hiltunen, J Kalervo, Michels, Paulus, Antonenkov, Vasily D, UCL - SSS/DDUV - Institut de Duve, Gualdron-López, Melisa, Vapola, Miia H, Miinalainen, Ilkka J, Hiltunen, J Kalervo, Michels, Paulus, and Antonenkov, Vasily D

Abstract

These results indicate that the membrane of glycosomes apparently contains several types of pore-forming channels connecting the glycosomal lumen and the cytosol.

Published
2012

Catalog

Books, media, physical & digital resources
See catalog results