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Background: Sport has high social valence and is a primary context for physical activity for the majority of youth. The aim of this study was to estimate the contribution of participation in organized sport to the total daily energy expenditure and also to its moderate-to-vigorous portion in male adolescents. Methods: The sample comprised 165 Portuguese male youth, aged 13 to 16 years. Physical activity was assessed with a multi-method approach (Actigraph GT1M accelerometer plus 3-day diary record). Differences in the intensities of physical activity and sedentary behavior of male sport participants and nonsport participants were compared using independent sample t-test. Results: Male participants in organized sports spent significantly more time in moderate-to-vigorous activities than nonparticipants, although the P-value for the 15 to 16 years age-group was marginal (P = .08) on the weekend days. In addition, male adolescents spent 11% to 13% of total daily energy expenditure in organized sports which corresponded to 35% to 42% of the moderate-to-vigorous portion of daily energy expenditure. Conclusion: Organized sport appears to be a relevant component of daily activity energy expenditure to promote healthy lifestyles among male adolescents.