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1,064 results on '"Rowan, A.E."'

1. Transcriptional comparison of testicular adrenal rest tumors with fetal and adult tissues

Author

Schröder, Mariska A.M., Sweep, F.C.G.J., Herwaarden, A.E. van, Mitchell, R.T., Eliveld, J., Pelt, A.M.M. van, Rowan, A.E., Korbie, D., Stikkelbroeck, N., Claahsen-van der Grinten, H.L., Span, P.N., Schröder, Mariska A.M., Sweep, F.C.G.J., Herwaarden, A.E. van, Mitchell, R.T., Eliveld, J., Pelt, A.M.M. van, Rowan, A.E., Korbie, D., Stikkelbroeck, N., Claahsen-van der Grinten, H.L., and Span, P.N.

Abstract

Item does not contain fulltext, BACKGROUND: Testicular adrenal rest tumors (TART) are a common complication of unknown cellular origin in patients with congenital adrenal hyperplasia (CAH). These benign tumors have both adrenal and testicular characteristics and are hypothesized to either derive from cells of adrenal origin from the fetal adrenogonadal primordium or by atypical differentiation of adult Leydig-progenitor cells. OBJECTIVE: This study aims to unravel the identity and etiology of TART. METHODS: Co-expression of adrenal-specific CYP11B1 and Leydig cell-specific HSD17B3 in TART was studied using immunohistochemistry. We studied the possibility of TART being derived from atypical differentiation of adult Leydig-progenitor cells by the quantification of adrenal-specific enzyme expression upon adrenocorticotrophic hormone (ACTH)-like stimulation of ex vivo cultured platelet-derived growth factor receptor alpha-positive cells. By comparing the transcriptome of TART (n = 16) with the transcriptome of fetal adrenal (n = 13), fetal testis (n = 5), adult adrenal (n = 11), and adult testis (n = 10) tissues, we explored the identity of TART. RESULTS: We demonstrate co-expression of adrenal-specific CYP11B1 and testis-specific HSD17B3 in TART cells, indicating the existence of a distinct TART cell exhibiting both adrenal and testicular characteristics. Ex vivo cultured adult Leydig-progenitor cells did not express the ACTH-receptor MC2R but did express CYP11B1 upon stimulation. Unsupervised clustering of transcriptome data showed that TART was most similar to adult adrenal tissue, followed by adult testis tissue, and least similar to either fetal tissue. CONCLUSION: Our data suggest that TART is induced - most likely via activation of a cAMP/protein kinase A-dependent receptor - from a progenitor cell into a unique mature adrenal-like cell type, sometimes exhibiting both adrenal and testicular features.

Published
2022

2. Transcriptional comparison of testicular adrenal rest tumors with fetal and adult tissues

Author

Schröder, Mariska A.M., Sweep, F.C.G.J., Herwaarden, A.E. van, Mitchell, R.T., Eliveld, J., Pelt, A.M.M. van, Rowan, A.E., Korbie, D., Stikkelbroeck, N., Claahsen-van der Grinten, H.L., Span, P.N., Schröder, Mariska A.M., Sweep, F.C.G.J., Herwaarden, A.E. van, Mitchell, R.T., Eliveld, J., Pelt, A.M.M. van, Rowan, A.E., Korbie, D., Stikkelbroeck, N., Claahsen-van der Grinten, H.L., and Span, P.N.

Abstract

Item does not contain fulltext, BACKGROUND: Testicular adrenal rest tumors (TART) are a common complication of unknown cellular origin in patients with congenital adrenal hyperplasia (CAH). These benign tumors have both adrenal and testicular characteristics and are hypothesized to either derive from cells of adrenal origin from the fetal adrenogonadal primordium or by atypical differentiation of adult Leydig-progenitor cells. OBJECTIVE: This study aims to unravel the identity and etiology of TART. METHODS: Co-expression of adrenal-specific CYP11B1 and Leydig cell-specific HSD17B3 in TART was studied using immunohistochemistry. We studied the possibility of TART being derived from atypical differentiation of adult Leydig-progenitor cells by the quantification of adrenal-specific enzyme expression upon adrenocorticotrophic hormone (ACTH)-like stimulation of ex vivo cultured platelet-derived growth factor receptor alpha-positive cells. By comparing the transcriptome of TART (n = 16) with the transcriptome of fetal adrenal (n = 13), fetal testis (n = 5), adult adrenal (n = 11), and adult testis (n = 10) tissues, we explored the identity of TART. RESULTS: We demonstrate co-expression of adrenal-specific CYP11B1 and testis-specific HSD17B3 in TART cells, indicating the existence of a distinct TART cell exhibiting both adrenal and testicular characteristics. Ex vivo cultured adult Leydig-progenitor cells did not express the ACTH-receptor MC2R but did express CYP11B1 upon stimulation. Unsupervised clustering of transcriptome data showed that TART was most similar to adult adrenal tissue, followed by adult testis tissue, and least similar to either fetal tissue. CONCLUSION: Our data suggest that TART is induced - most likely via activation of a cAMP/protein kinase A-dependent receptor - from a progenitor cell into a unique mature adrenal-like cell type, sometimes exhibiting both adrenal and testicular features.

Published
2022

3. Magnetic anisotropy of individually addressed spin states

Author

Peters, L. C. J. M., Christianen, P.C.M., Engelkamp, H., Groenenboom, G.C., Maan, J.C., Kampert, W.A.G., Rowan, A.E., Zeitler, U., Peters, L. C. J. M., Christianen, P.C.M., Engelkamp, H., Groenenboom, G.C., Maan, J.C., Kampert, W.A.G., Rowan, A.E., and Zeitler, U.

Abstract

Contains fulltext : 244646.pdf (Publisher’s version ) (Open Access)

Published
2021

7. Synthesis and Magnetic Properties of Two-Step-Coordination Schiff Base Clusters

Author

Peters, L.C.J.M., Engelkamp, H., Zeitler, U., Christianen, P.C.M., Tinnemans, P., Maan, J.C., Rowan, A.E., Peters, L.C.J.M., Engelkamp, H., Zeitler, U., Christianen, P.C.M., Tinnemans, P., Maan, J.C., and Rowan, A.E.

Abstract

Contains fulltext : 235688.pdf (Publisher’s version ) (Open Access)

Published
2021

8. Magnetic anisotropy of individually addressed spin states

Author

Peters, L. C. J. M., Christianen, P.C.M., Engelkamp, H., Groenenboom, G.C., Maan, J.C., Kampert, W.A.G., Rowan, A.E., Zeitler, U., Peters, L. C. J. M., Christianen, P.C.M., Engelkamp, H., Groenenboom, G.C., Maan, J.C., Kampert, W.A.G., Rowan, A.E., and Zeitler, U.

Abstract

Contains fulltext : 244646.pdf (Publisher’s version ) (Open Access)

Published
2021

10. Polyisocyanide Hydrogels as a Tunable Platform for Mammary Gland Organoid Formation

Author

Zhang, Y., Tang, C., Span, P.N., Rowan, A.E., Aalders, T., Schalken, J.A., Adema, G.J., Kouwer, P.H.J., Zegers, M.M.P., Ansems, M., Zhang, Y., Tang, C., Span, P.N., Rowan, A.E., Aalders, T., Schalken, J.A., Adema, G.J., Kouwer, P.H.J., Zegers, M.M.P., and Ansems, M.

Abstract

Contains fulltext : 227470.pdf (publisher's version ) (Open Access)

Published
2020

12. Double Porphyrin Cage Compounds

Author

Stout, K., Peters, T.P.J., Mabesoone, M.F.J., Visschers, F.L.L., Meijer, E.M, Klop, J., Rowan, A.E., Nolte, R.J.M., Elemans, J.A.A.W., Stout, K., Peters, T.P.J., Mabesoone, M.F.J., Visschers, F.L.L., Meijer, E.M, Klop, J., Rowan, A.E., Nolte, R.J.M., and Elemans, J.A.A.W.

Abstract

Contains fulltext : 227130.pdf (publisher's version ) (Open Access)

Published
2020

13. Polyisocyanide Hydrogels as a Tunable Platform for Mammary Gland Organoid Formation

Author

Zhang, Y., Tang, C., Span, P.N., Rowan, A.E., Aalders, T., Schalken, J.A., Adema, G.J., Kouwer, P.H.J., Zegers, M.M.P., Ansems, M., Zhang, Y., Tang, C., Span, P.N., Rowan, A.E., Aalders, T., Schalken, J.A., Adema, G.J., Kouwer, P.H.J., Zegers, M.M.P., and Ansems, M.

Abstract

Contains fulltext : 227470.pdf (publisher's version ) (Open Access)

Published
2020

15. Double Porphyrin Cage Compounds

Author

Stout, K., Peters, T.P.J., Mabesoone, M.F.J., Visschers, F.L.L., Meijer, E.M, Klop, J., Rowan, A.E., Nolte, R.J.M., Elemans, J.A.A.W., Stout, K., Peters, T.P.J., Mabesoone, M.F.J., Visschers, F.L.L., Meijer, E.M, Klop, J., Rowan, A.E., Nolte, R.J.M., and Elemans, J.A.A.W.

Abstract

Contains fulltext : 227130.pdf (publisher's version ) (Open Access)

Published
2020

16. Double Porphyrin Cage Compounds

Author

Stout, K., Peters, T.P.J., Mabesoone, M.F.J., Visschers, F.L.L., Meijer, E.M, Klop, J., Rowan, A.E., Nolte, R.J.M., Elemans, J.A.A.W., Stout, K., Peters, T.P.J., Mabesoone, M.F.J., Visschers, F.L.L., Meijer, E.M, Klop, J., Rowan, A.E., Nolte, R.J.M., and Elemans, J.A.A.W.

Abstract

Contains fulltext : 227130.pdf (publisher's version ) (Open Access)

Published
2020

17. Polyisocyanide Hydrogels as a Tunable Platform for Mammary Gland Organoid Formation

Author

Zhang, Y., Tang, C., Span, P.N., Rowan, A.E., Aalders, T., Schalken, J.A., Adema, G.J., Kouwer, P.H.J., Zegers, M.M.P., Ansems, M., Zhang, Y., Tang, C., Span, P.N., Rowan, A.E., Aalders, T., Schalken, J.A., Adema, G.J., Kouwer, P.H.J., Zegers, M.M.P., and Ansems, M.

Abstract

Contains fulltext : 227470.pdf (publisher's version ) (Open Access)

Published
2020

18. Epitaxial Crystallization of Insulin on an Ordered 2D Polymer Template

Author

Poel, Wester de, Elemans, J.A.A.W., Enckevort, Willem J.P. van, Rowan, A.E., Vlieg, Elias, Poel, Wester de, Elemans, J.A.A.W., Enckevort, Willem J.P. van, Rowan, A.E., and Vlieg, Elias

Abstract

Contains fulltext : 202332.pdf (publisher's version ) (Closed access)

Published
2019

19. Cytoskeletal stiffening in synthetic hydrogels

Author

Almeida, P. de, Jaspers, M., Vaessen, Sarah L., Tagit, O., Portale, G., Rowan, A.E., Kouwer, P.H.J., Almeida, P. de, Jaspers, M., Vaessen, Sarah L., Tagit, O., Portale, G., Rowan, A.E., and Kouwer, P.H.J.

Abstract

Contains fulltext : 201184.pdf (publisher's version ) (Open Access)

Published
2019

20. Monitoring (111)In-labelled polyisocyanopeptide (PIC) hydrogel wound dressings in full-thickness wounds

Author

Veld, R.C. op 't, Joosten, L., Boomen, O.I. van den, Boerman, O.C., Kouwer, P.H.J., Middelkoop, E., Rowan, A.E., Jansen, J.A., Walboomers, X.F., Wagener, F.A.D.T.G., Veld, R.C. op 't, Joosten, L., Boomen, O.I. van den, Boerman, O.C., Kouwer, P.H.J., Middelkoop, E., Rowan, A.E., Jansen, J.A., Walboomers, X.F., and Wagener, F.A.D.T.G.

Abstract

Contains fulltext : 205348.pdf (publisher's version ) (Closed access), Wounds often result in scarring, prolonged morbidity, and loss of function. New interactive and modifiable hydrogel wound dressings are being developed for these injuries. Polyisocyanopeptide (PIC) gel is a promising thermosensitive hydrogel having several characteristics that can facilitate wound repair, including ease of application/removal and strain-stiffening properties that mimic extracellular matrix components. However, it is unknown whether the PIC gel remains in the wound for a clinically relevant time period. Therefore, PIC polymers were functionalized with a DTPA group allowing labelling with Indium-111 (111In). Following application of this radiolabelled gel to splinted and non-splinted murine full-thickness skin wounds the signal was monitored using SPECT/CT imaging for 7 days. The SPECT signal from the PIC gel was highly stable and covered the complete wound area. Non-bound 111In-EDTA was rapidly cleared via the kidneys to the urine. The impact of PIC gels on wound repair was further studied visually and histologically. Radiolabelled PIC gel was observed to move both over and under the skin, while histological analysis demonstrated that part of the gel became encapsulated within the wound repair tissue, but did not delay wound closure or otherwise impair wound healing. This work illustrates for the first time the use of 111In-labelled PIC gels for diagnostic and monitoring purposes and describes the use of PIC in the (non-)splinted murine skin wound model. It was found that PIC gels remained in splinted and non-splinted full-thickness skin wounds during wound repair. This warrants the continuation of developing the PIC gel into a clinically advanced wound dressing.

Published
2019

21. Synthetic Semiflexible and Bioactive Brushes

Author

Voerman, D., Schluck, M., Weiden, J., Joosten, B.H., Eggermont, L.J., Eijnde, Tuur van den, Ignacio, B.J., Cambi, A., Figdor, C.G., Kouwer, P.H.J., Verdoes, M., Hammink, R., Rowan, A.E., Voerman, D., Schluck, M., Weiden, J., Joosten, B.H., Eggermont, L.J., Eijnde, Tuur van den, Ignacio, B.J., Cambi, A., Figdor, C.G., Kouwer, P.H.J., Verdoes, M., Hammink, R., and Rowan, A.E.

Abstract

Contains fulltext : 206170.pdf (publisher's version ) (Open Access)

Published
2019

24. Epitaxial Crystallization of Insulin on an Ordered 2D Polymer Template

Author

Poel, Wester de, Elemans, J.A.A.W., Enckevort, Willem J.P. van, Rowan, A.E., Vlieg, Elias, Poel, Wester de, Elemans, J.A.A.W., Enckevort, Willem J.P. van, Rowan, A.E., and Vlieg, Elias

Abstract

Contains fulltext : 202332.pdf (publisher's version ) (Closed access)

Published
2019

25. Cytoskeletal stiffening in synthetic hydrogels

Author

Almeida, P. de, Jaspers, M., Vaessen, Sarah L., Tagit, O., Portale, G., Rowan, A.E., Kouwer, P.H.J., Almeida, P. de, Jaspers, M., Vaessen, Sarah L., Tagit, O., Portale, G., Rowan, A.E., and Kouwer, P.H.J.

Abstract

Contains fulltext : 201184.pdf (publisher's version ) (Open Access)

Published
2019

26. Monitoring (111)In-labelled polyisocyanopeptide (PIC) hydrogel wound dressings in full-thickness wounds

Author

Veld, R.C. op 't, Joosten, L., Boomen, O.I. van den, Boerman, O.C., Kouwer, P.H.J., Middelkoop, E., Rowan, A.E., Jansen, J.A., Walboomers, X.F., Wagener, F.A.D.T.G., Veld, R.C. op 't, Joosten, L., Boomen, O.I. van den, Boerman, O.C., Kouwer, P.H.J., Middelkoop, E., Rowan, A.E., Jansen, J.A., Walboomers, X.F., and Wagener, F.A.D.T.G.

Abstract

Contains fulltext : 205348.pdf (Publisher’s version ) (Open Access), Wounds often result in scarring, prolonged morbidity, and loss of function. New interactive and modifiable hydrogel wound dressings are being developed for these injuries. Polyisocyanopeptide (PIC) gel is a promising thermosensitive hydrogel having several characteristics that can facilitate wound repair, including ease of application/removal and strain-stiffening properties that mimic extracellular matrix components. However, it is unknown whether the PIC gel remains in the wound for a clinically relevant time period. Therefore, PIC polymers were functionalized with a DTPA group allowing labelling with Indium-111 (111In). Following application of this radiolabelled gel to splinted and non-splinted murine full-thickness skin wounds the signal was monitored using SPECT/CT imaging for 7 days. The SPECT signal from the PIC gel was highly stable and covered the complete wound area. Non-bound 111In-EDTA was rapidly cleared via the kidneys to the urine. The impact of PIC gels on wound repair was further studied visually and histologically. Radiolabelled PIC gel was observed to move both over and under the skin, while histological analysis demonstrated that part of the gel became encapsulated within the wound repair tissue, but did not delay wound closure or otherwise impair wound healing. This work illustrates for the first time the use of 111In-labelled PIC gels for diagnostic and monitoring purposes and describes the use of PIC in the (non-)splinted murine skin wound model. It was found that PIC gels remained in splinted and non-splinted full-thickness skin wounds during wound repair. This warrants the continuation of developing the PIC gel into a clinically advanced wound dressing.

Published
2019

27. Synthetic Semiflexible and Bioactive Brushes

Author

Voerman, D., Schluck, M., Weiden, J., Joosten, B.H., Eggermont, L.J., Eijnde, Tuur van den, Ignacio, B.J., Cambi, A., Figdor, C.G., Kouwer, P.H.J., Verdoes, M., Hammink, R., Rowan, A.E., Voerman, D., Schluck, M., Weiden, J., Joosten, B.H., Eggermont, L.J., Eijnde, Tuur van den, Ignacio, B.J., Cambi, A., Figdor, C.G., Kouwer, P.H.J., Verdoes, M., Hammink, R., and Rowan, A.E.

Abstract

Contains fulltext : 206170.pdf (publisher's version ) (Open Access)

Published
2019

29. Epitaxial Crystallization of Insulin on an Ordered 2D Polymer Template

Author

Poel, Wester de, Elemans, J.A.A.W., Enckevort, Willem J.P. van, Rowan, A.E., Vlieg, Elias, Poel, Wester de, Elemans, J.A.A.W., Enckevort, Willem J.P. van, Rowan, A.E., and Vlieg, Elias

Abstract

Contains fulltext : 202332.pdf (publisher's version ) (Closed access)

Published
2019

30. Cytoskeletal stiffening in synthetic hydrogels

Author

Almeida, P. de, Jaspers, M., Vaessen, Sarah L., Tagit, O., Portale, G., Rowan, A.E., Kouwer, P.H.J., Almeida, P. de, Jaspers, M., Vaessen, Sarah L., Tagit, O., Portale, G., Rowan, A.E., and Kouwer, P.H.J.

Abstract

Contains fulltext : 201184.pdf (publisher's version ) (Open Access)

Published
2019

31. Monitoring (111)In-labelled polyisocyanopeptide (PIC) hydrogel wound dressings in full-thickness wounds

Author

Veld, R.C. op 't, Joosten, L., Boomen, O.I. van den, Boerman, O.C., Kouwer, P.H.J., Middelkoop, E., Rowan, A.E., Jansen, J.A., Walboomers, X.F., Wagener, F.A.D.T.G., Veld, R.C. op 't, Joosten, L., Boomen, O.I. van den, Boerman, O.C., Kouwer, P.H.J., Middelkoop, E., Rowan, A.E., Jansen, J.A., Walboomers, X.F., and Wagener, F.A.D.T.G.

Abstract

Contains fulltext : 205348.pdf (Publisher’s version ) (Open Access), Wounds often result in scarring, prolonged morbidity, and loss of function. New interactive and modifiable hydrogel wound dressings are being developed for these injuries. Polyisocyanopeptide (PIC) gel is a promising thermosensitive hydrogel having several characteristics that can facilitate wound repair, including ease of application/removal and strain-stiffening properties that mimic extracellular matrix components. However, it is unknown whether the PIC gel remains in the wound for a clinically relevant time period. Therefore, PIC polymers were functionalized with a DTPA group allowing labelling with Indium-111 (111In). Following application of this radiolabelled gel to splinted and non-splinted murine full-thickness skin wounds the signal was monitored using SPECT/CT imaging for 7 days. The SPECT signal from the PIC gel was highly stable and covered the complete wound area. Non-bound 111In-EDTA was rapidly cleared via the kidneys to the urine. The impact of PIC gels on wound repair was further studied visually and histologically. Radiolabelled PIC gel was observed to move both over and under the skin, while histological analysis demonstrated that part of the gel became encapsulated within the wound repair tissue, but did not delay wound closure or otherwise impair wound healing. This work illustrates for the first time the use of 111In-labelled PIC gels for diagnostic and monitoring purposes and describes the use of PIC in the (non-)splinted murine skin wound model. It was found that PIC gels remained in splinted and non-splinted full-thickness skin wounds during wound repair. This warrants the continuation of developing the PIC gel into a clinically advanced wound dressing.

Published
2019

32. Synthetic Semiflexible and Bioactive Brushes

Author

Voerman, D., Schluck, M., Weiden, J., Joosten, B.H., Eggermont, L.J., Eijnde, Tuur van den, Ignacio, B.J., Cambi, A., Figdor, C.G., Kouwer, P.H.J., Verdoes, M., Hammink, R., Rowan, A.E., Voerman, D., Schluck, M., Weiden, J., Joosten, B.H., Eggermont, L.J., Eijnde, Tuur van den, Ignacio, B.J., Cambi, A., Figdor, C.G., Kouwer, P.H.J., Verdoes, M., Hammink, R., and Rowan, A.E.

Abstract

Contains fulltext : 206170.pdf (publisher's version ) (Open Access)

Published
2019

33. Controlling the gelation temperature of biomimetic polyisocyanides

Author

Kouwer, P.H.J., Almeida, P. de, Boomen, O.I. van den, Eksteen-Akeroyd, Z.H., Hammink, R., Jaspers, M., Kragt, S., Mabesoone, M.F., Nolte, R.J.M., Rowan, A.E., Rutten, M.G.T.A., Sage, V.A.A. le, Schoenmakers, D.C., Xing, C., Xu, J., Kouwer, P.H.J., Almeida, P. de, Boomen, O.I. van den, Eksteen-Akeroyd, Z.H., Hammink, R., Jaspers, M., Kragt, S., Mabesoone, M.F., Nolte, R.J.M., Rowan, A.E., Rutten, M.G.T.A., Sage, V.A.A. le, Schoenmakers, D.C., Xing, C., and Xu, J.

Abstract

Contains fulltext : 183344.pdf (author's version ) (Open Access) Contains fulltext : 183344pub.pdf (publisher's version ) (Closed access)

Published
2018

34. Functional Crosslinks in Synthetic Biomimetic Networks

Author

Rowan, A.E., Kouwer, P.H.J., Schoenmakers, D.C., Rowan, A.E., Kouwer, P.H.J., and Schoenmakers, D.C.

Abstract

Radboud University, 21 september 2018, Promotor : Rowan, A.E. Co-promotor : Kouwer, P.H.J., Contains fulltext : 195018.pdf (publisher's version ) (Open Access)

Published
2018

36. Water-Soluble Conjugated Polymers Composites for Chemo-/Biosensing

Author

Rowan, A.E., Zhan, Y., Xing, C., Kouwer, P.H.J., Yuan, H., Rowan, A.E., Zhan, Y., Xing, C., Kouwer, P.H.J., and Yuan, H.

Abstract

Radboud University, 05 februari 2018, Promotores : Rowan, A.E., Zhan, Y., Xing, C. Co-promotor : Kouwer, P.H.J., Contains fulltext : 180514.pdf (publisher's version ) (Open Access)

Published
2018

37. Crosslinking of fibrous hydrogels

Author

Schoenmakers, D.C., Rowan, A.E., Kouwer, P.H.J., Schoenmakers, D.C., Rowan, A.E., and Kouwer, P.H.J.

Abstract

Contains fulltext : 192101.pdf (publisher's version ) (Open Access)

Published
2018

38. Thermosensitive biomimetic polyisocyanopeptide hydrogels may facilitate wound repair.

Author

Veld, R.C. op 't, Boomen, O.I. van den, Lundvig, D.M.S., Bronkhorst, E.M., Kouwer, P.H.J., Jansen, J.A., Middelkoop, E., Hoff, J.W. Von den, Rowan, A.E., Wagener, F.A.D.T.G., Veld, R.C. op 't, Boomen, O.I. van den, Lundvig, D.M.S., Bronkhorst, E.M., Kouwer, P.H.J., Jansen, J.A., Middelkoop, E., Hoff, J.W. Von den, Rowan, A.E., and Wagener, F.A.D.T.G.

Abstract

1 oktober 2018, Contains fulltext : 196295.pdf (publisher's version ) (Closed access), Changing wound dressings inflicts pain and may disrupt wound repair. Novel synthetic thermosensitive hydrogels based on polyisocyanopeptide (PIC) offer a solution. These gels are liquid below 16 degrees C and form gels beyond room temperature. The architecture and mechanical properties of PIC gels closely resemble collagen and fibrin, and include the characteristic stiffening response at high strains. Considering the reversible thermo-responsive behavior, we postulate that PIC gels are easy to apply and remove, and facilitate healing without eliciting foreign body responses or excessive inflammation. Biocompatibility may be higher in RGD-peptide-functionalized PIC gels due to enhanced cell binding capabilities. Full-thickness dorsal skin wounds in mice were compared to wounds treated with PIC gel and PIC-RGD gel for 3 and 7 days. No foreign body reactions and similar wound closure rates were found in all groups. The level of macrophages, myofibroblasts, epithelial migration, collagen expression, and blood vessels did not significantly differ from controls. Surprisingly, granulocyte populations in the wound decreased significantly in the PIC gel-treated groups, likely because foreign bacteria could not penetrate the gel. RGD-peptides did not further improve any effect observed for PIC. The absence of adverse effects, ease of application, and the possibilities for bio-functionalization make the biomimetic PIC hydrogels suitable for development into wound dressings.

Published
2018

39. Strong optical nonlinearities of self-assembled polymorphic microstructures of phenylethynyl functionalized fluorenones

Author

Li, X., Semin, Sergey, Estrada, Leandro A., Yuan, C.Q., Duan, Y., Cremers, J., Tinnemans, P.T., Kouwer, Paul, Rowan, A.E., Rasing, Theo, Xu, Jialiang, Li, X., Semin, Sergey, Estrada, Leandro A., Yuan, C.Q., Duan, Y., Cremers, J., Tinnemans, P.T., Kouwer, Paul, Rowan, A.E., Rasing, Theo, and Xu, Jialiang

Abstract

Contains fulltext : 200409.pdf (Publisher’s version ) (Open Access)

Published
2018

40. Polyisocyanopeptide hydrogels: A novel thermo-responsive hydrogel supporting pre-vascularization and the development of organotypic structures

Author

Zimoch, Jakub, Simo Padial, P., Klar, Agnes S., Vallmajo-Martin, Queralt, Meuli, Martin, Biedermann, Thomas, Rowan, A.E., Reichmann, Ernst, Zimoch, Jakub, Simo Padial, P., Klar, Agnes S., Vallmajo-Martin, Queralt, Meuli, Martin, Biedermann, Thomas, Rowan, A.E., and Reichmann, Ernst

Abstract

Contains fulltext : 191187.pdf (publisher's version ) (Open Access)

Published
2018

41. Crosslinking of fibrous hydrogels

Author

Schoenmakers, D.C., Rowan, A.E., Kouwer, P.H.J., Schoenmakers, D.C., Rowan, A.E., and Kouwer, P.H.J.

Abstract

Contains fulltext : 192101.pdf (publisher's version ) (Open Access)

Published
2018

44. Controlling the gelation temperature of biomimetic polyisocyanides

Author

Kouwer, P.H.J., Almeida, P. de, Boomen, O.I. van den, Eksteen-Akeroyd, Z.H., Hammink, R., Jaspers, M., Kragt, S., Mabesoone, M.F., Nolte, R.J.M., Rowan, A.E., Rutten, M.G.T.A., Sage, V.A.A. le, Schoenmakers, D.C., Xing, C., Xu, J., Kouwer, P.H.J., Almeida, P. de, Boomen, O.I. van den, Eksteen-Akeroyd, Z.H., Hammink, R., Jaspers, M., Kragt, S., Mabesoone, M.F., Nolte, R.J.M., Rowan, A.E., Rutten, M.G.T.A., Sage, V.A.A. le, Schoenmakers, D.C., Xing, C., and Xu, J.

Abstract

Contains fulltext : 183344pub.pdf (Publisher’s version ) (Open Access) Contains fulltext : 183344.pdf (author's version ) (Open Access)

Published
2018

45. Functional Crosslinks in Synthetic Biomimetic Networks

Author

Rowan, A.E., Kouwer, P.H.J., Schoenmakers, D.C., Rowan, A.E., Kouwer, P.H.J., and Schoenmakers, D.C.

Abstract

Radboud University, 21 september 2018, Promotor : Rowan, A.E. Co-promotor : Kouwer, P.H.J., Contains fulltext : 195018.pdf (publisher's version ) (Open Access)

Published
2018

47. Water-Soluble Conjugated Polymers Composites for Chemo-/Biosensing

Author

Rowan, A.E., Zhan, Y., Xing, C., Kouwer, P.H.J., Yuan, H., Rowan, A.E., Zhan, Y., Xing, C., Kouwer, P.H.J., and Yuan, H.

Abstract

Radboud University, 05 februari 2018, Promotores : Rowan, A.E., Zhan, Y., Xing, C. Co-promotor : Kouwer, P.H.J., Contains fulltext : 180514.pdf (publisher's version ) (Open Access)

Published
2018

48. Crosslinking of fibrous hydrogels

Author

Schoenmakers, D.C., Rowan, A.E., Kouwer, P.H.J., Schoenmakers, D.C., Rowan, A.E., and Kouwer, P.H.J.

Abstract

Contains fulltext : 192101.pdf (publisher's version ) (Open Access)

Published
2018

49. Polyisocyanopeptide hydrogels: A novel thermo-responsive hydrogel supporting pre-vascularization and the development of organotypic structures

Author

Zimoch, Jakub, Simo Padial, P., Klar, Agnes S., Vallmajo-Martin, Queralt, Meuli, Martin, Biedermann, Thomas, Wilson, Christopher J., Rowan, A.E., Reichmann, Ernst, Zimoch, Jakub, Simo Padial, P., Klar, Agnes S., Vallmajo-Martin, Queralt, Meuli, Martin, Biedermann, Thomas, Wilson, Christopher J., Rowan, A.E., and Reichmann, Ernst

Abstract

Contains fulltext : 191187.pdf (publisher's version ) (Open Access)

Published
2018

50. Thermosensitive biomimetic polyisocyanopeptide hydrogels may facilitate wound repair.

Author

Veld, R.C. op 't, Boomen, O.I. van den, Lundvig, D.M.S., Bronkhorst, E.M., Kouwer, P.H.J., Jansen, J.A., Middelkoop, E., Hoff, J.W. Von den, Rowan, A.E., Wagener, F.A.D.T.G., Veld, R.C. op 't, Boomen, O.I. van den, Lundvig, D.M.S., Bronkhorst, E.M., Kouwer, P.H.J., Jansen, J.A., Middelkoop, E., Hoff, J.W. Von den, Rowan, A.E., and Wagener, F.A.D.T.G.

Abstract

01 oktober 2018, Contains fulltext : 196295.pdf (Publisher’s version ) (Open Access), Changing wound dressings inflicts pain and may disrupt wound repair. Novel synthetic thermosensitive hydrogels based on polyisocyanopeptide (PIC) offer a solution. These gels are liquid below 16 degrees C and form gels beyond room temperature. The architecture and mechanical properties of PIC gels closely resemble collagen and fibrin, and include the characteristic stiffening response at high strains. Considering the reversible thermo-responsive behavior, we postulate that PIC gels are easy to apply and remove, and facilitate healing without eliciting foreign body responses or excessive inflammation. Biocompatibility may be higher in RGD-peptide-functionalized PIC gels due to enhanced cell binding capabilities. Full-thickness dorsal skin wounds in mice were compared to wounds treated with PIC gel and PIC-RGD gel for 3 and 7 days. No foreign body reactions and similar wound closure rates were found in all groups. The level of macrophages, myofibroblasts, epithelial migration, collagen expression, and blood vessels did not significantly differ from controls. Surprisingly, granulocyte populations in the wound decreased significantly in the PIC gel-treated groups, likely because foreign bacteria could not penetrate the gel. RGD-peptides did not further improve any effect observed for PIC. The absence of adverse effects, ease of application, and the possibilities for bio-functionalization make the biomimetic PIC hydrogels suitable for development into wound dressings.

Published
2018

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