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Aim Orthodontic treatment need indexes are indispensable tools for collecting aepidemiological information, to define the need for clinical intervention on a graduated risk scale and to monitor the evolution of clinical conditions over time. The purpose of this research is to evaluate the prevalence of malocclusion and associated risk factors, and to compare the prevalence of malocclusion related to children’s growth. Investigating the correlation between malocclusion and dental occlusion development is crucial to define orthodontic timing and the most suitable treatment modalities. Materials and methods A cross-sectional, multi-center, observational study was carried out throughout Italy: 4,422 patients aged between 2 and 13 years were visited. The patients were divided into two age groups: one composed of patients aged 2 -7 years, and one of children between 8 and 13 years of age. The prevalence data were classified according to the items of the Baby ROMA (first group, deciduous/early mixed dentition) and ROMA Index (second group, late mixed/permanent dentition). Pearson’s Chi-square test was applied for the calculation of statistical significance in the comparison of prevalence data between the two age groups (P <0.05). Results The need for orthodontic treatment among Italian children is high. In the sample aged between 2 and 7 years, the most frequent malocclusions are the moderate and severe open bite (23%), moderate and severe Class II (21.2%), deep bite (18.8%), cross bite (16.5%), Class III (7.7%), and crowding (5.1%). In the sample aged between 8 and 13 years the most frequent malocclusions are crowding (50.8 %), Class II (33.1%), deep bite (19.2%) crossbite (18.1%), Class III (9.1%), open bite (6.6%), facial or mandibular asymmetries (3.3%), and functional asymmetries (3.2%). It was found a statistically significant variation of the different malocclusion conditions according to age. Conclusions The study confirms that some risk factors occur during gro

Diseases of the descending aorta have emerged as a clinical issue in Marfan syndrome following improvements in proximal aorta surgical treatment and the consequent increase in life expectancy. Although a role for hemodynamic alterations in the etiology of descending aorta disease in Marfan patients has been suggested, whether flow characteristics may be useful as early markers remains to be determined. Seventy-five Marfan patients and 48 healthy subjects were prospectively enrolled. In- and through-plane vortexes were computed by 4D flow cardiovascular magnetic resonance (CMR) in the thoracic aorta through the quantification of in-plane rotational flow and systolic flow reversal ratio, respectively. Regional pulse wave velocity and axial and circumferential wall shear stress maps were also computed. In-plane rotational flow and circumferential wall shear stress were reduced in Marfan patients in the distal ascending aorta and in proximal descending aorta, even in the 20 patients free of aortic dilation. Multivariate analysis showed reduced in-plane rotational flow to be independently related to descending aorta pulse wave velocity. Conversely, systolic flow reversal ratio and axial wall shear stress were altered in unselected Marfan patients but not in the subgroup without dilation. In multivariate regression analysis proximal descending aorta axial (p = 0.014) and circumferential (p = 0.034) wall shear stress were independently related to local diameter. Reduced rotational flow is present in the aorta of Marfan patients even in the absence of dilation, is related to aortic stiffness and drives abnormal circumferential wall shear stress. Axial and circumferential wall shear stress are independently related to proximal descending aorta dilation beyond clinical factors. In-plane rotational flow and circumferential wall shear stress may be considered as an early marker of descending aorta dilation in Marfan patients. The online version of this article (10.1186/s12968-0

Aims: Exercise-derived parameters, specifically peak exercise oxygen uptake (peak VO 2 ) and minute ventilation/carbon dioxide relationship slope (VE/VCO 2 slope), have a pivotal prognostic value in heart failure (HF). It is unknown how the prognostic threshold of peak VO 2 and VE/VCO 2 slope has changed over the last 20 years in parallel with HF prognosis improvement. Methods and results: Data from 6083 HF patients (81% male, age 61 ± 13 years), enrolled in the MECKI score database between 1993 and 2015, were retrospectively analysed. By enrolment year, four groups were generated: group 1 1993–2000 (n = 440), group 2 2001–2005 (n = 1288), group 3 2006–2010 (n = 2368), and group 4 2011–2015 (n = 1987). We compared the 10-year survival of groups and analysed how the overall risk (cardiovascular death, urgent heart transplantation, or left ventricular assist device implantation) changed over time according to peak VO 2 and VE/VCO 2 slope and to major clinical and therapeutic variables. At 10 years, a progressively higher survival from group 1 to group 3 was observed, with no further improvement afterwards. A 20% risk for peak VO 2 15 mL/min/kg (95% confidence interval 16–13), 9 (11–8), 4 (4–2) and 5 (7–4) was observed in group 1, 2, 3, and 4, respectively, while the VE/VCO 2 slope value for a 20% risk was 32 (37–29), 47 (51–43), 59 (64–55), and 57 (63–52), respectively. Conclusions: Heart failure prognosis improved over time up to 2010 in a HF population followed by experienced centres. The peak VO 2 and VE/VCO 2 slope cut-offs identifying a definite risk progressively decreased and increased over time, respectively. The prognostic threshold of peak VO 2 and VE/VCO 2 slope must be updated whenever HF prognosis improves.

Contains fulltext : 165650.PDF (publisher's version ) (Open Access), BACKGROUND: The global burden of pediatric severe respiratory illness is substantial, and influenza viruses contribute to this burden. Systematic surveillance and testing for influenza among hospitalized children has expanded globally over the past decade. However, only a fraction of the data has been used to estimate influenza burden. In this analysis, we use surveillance data to provide an estimate of influenza-associated hospitalizations among children worldwide. METHODS AND FINDINGS: We aggregated data from a systematic review (n = 108) and surveillance platforms (n = 37) to calculate a pooled estimate of the proportion of samples collected from children hospitalized with respiratory illnesses and positive for influenza by age group (<6 mo, <1 y, <2 y, <5 y, 5-17 y, and <18 y). We applied this proportion to global estimates of acute lower respiratory infection hospitalizations among children aged <1 y and <5 y, to obtain the number and per capita rate of influenza-associated hospitalizations by geographic region and socio-economic status. Influenza was associated with 10% (95% CI 8%-11%) of respiratory hospitalizations in children <18 y worldwide, ranging from 5% (95% CI 3%-7%) among children <6 mo to 16% (95% CI 14%-20%) among children 5-17 y. On average, we estimated that influenza results in approximately 374,000 (95% CI 264,000 to 539,000) hospitalizations in children <1 y-of which 228,000 (95% CI 150,000 to 344,000) occur in children <6 mo-and 870,000 (95% CI 610,000 to 1,237,000) hospitalizations in children <5 y annually. Influenza-associated hospitalization rates were more than three times higher in developing countries than in industrialized countries (150/100,000 children/year versus 48/100,000). However, differences in hospitalization practices between settings are an important limitation in interpreting these findings. CONCLUSIONS: Influenza is an important contributor to respiratory hospitalizations among young children worldwide. Increasing influen

The Penman–Monteith equation has been widely used to estimate the maximum evaporation rate (E) from wet/saturated forest canopies, regardless of canopy cover fraction. Forests are then represented as a big leaf and interception loss considered essentially as a one-dimensional process. With increasing forest sparseness the assumptions behind this big leaf approach become questionable. In sparse forests it might be better to model E and interception loss at the tree level assuming that the individual tree crowns behave as wet bulbs (‘‘wet bulb approach”). In this study, and for five different forest types and climate conditions, interception loss measurements were compared to modelled values (Gash’s interception model) based on estimates of E by the Penman–Monteith and the wet bulb approaches. Results show that the wet bulb approach is a good, and less data demanding, alternative to estimate E when the forest canopy is fully ventilated (very sparse forests with a narrow canopy depth). When the canopy is not fully ventilated, the wet bulb approach requires a reduction of leaf area index to the upper, more ventilated parts of the canopy, needing data on the vertical leaf area distribution, which is seldom-available. In such cases, the Penman–Monteith approach seems preferable. Our data also show that canopy cover does not per se allow us to identify if a forest canopy is fully ventilated or not. New methodologies of sensitivity analyses applied to Gash’s model showed that a correct estimate of E is critical for the proper modelling of interception loss.

Contains fulltext : 165650.PDF (publisher's version ) (Open Access), BACKGROUND: The global burden of pediatric severe respiratory illness is substantial, and influenza viruses contribute to this burden. Systematic surveillance and testing for influenza among hospitalized children has expanded globally over the past decade. However, only a fraction of the data has been used to estimate influenza burden. In this analysis, we use surveillance data to provide an estimate of influenza-associated hospitalizations among children worldwide. METHODS AND FINDINGS: We aggregated data from a systematic review (n = 108) and surveillance platforms (n = 37) to calculate a pooled estimate of the proportion of samples collected from children hospitalized with respiratory illnesses and positive for influenza by age group (<6 mo, <1 y, <2 y, <5 y, 5-17 y, and <18 y). We applied this proportion to global estimates of acute lower respiratory infection hospitalizations among children aged <1 y and <5 y, to obtain the number and per capita rate of influenza-associated hospitalizations by geographic region and socio-economic status. Influenza was associated with 10% (95% CI 8%-11%) of respiratory hospitalizations in children <18 y worldwide, ranging from 5% (95% CI 3%-7%) among children <6 mo to 16% (95% CI 14%-20%) among children 5-17 y. On average, we estimated that influenza results in approximately 374,000 (95% CI 264,000 to 539,000) hospitalizations in children <1 y-of which 228,000 (95% CI 150,000 to 344,000) occur in children <6 mo-and 870,000 (95% CI 610,000 to 1,237,000) hospitalizations in children <5 y annually. Influenza-associated hospitalization rates were more than three times higher in developing countries than in industrialized countries (150/100,000 children/year versus 48/100,000). However, differences in hospitalization practices between settings are an important limitation in interpreting these findings. CONCLUSIONS: Influenza is an important contributor to respiratory hospitalizations among young children worldwide. Increasing influen

Contains fulltext : 165650.PDF (publisher's version ) (Open Access), BACKGROUND: The global burden of pediatric severe respiratory illness is substantial, and influenza viruses contribute to this burden. Systematic surveillance and testing for influenza among hospitalized children has expanded globally over the past decade. However, only a fraction of the data has been used to estimate influenza burden. In this analysis, we use surveillance data to provide an estimate of influenza-associated hospitalizations among children worldwide. METHODS AND FINDINGS: We aggregated data from a systematic review (n = 108) and surveillance platforms (n = 37) to calculate a pooled estimate of the proportion of samples collected from children hospitalized with respiratory illnesses and positive for influenza by age group (<6 mo, <1 y, <2 y, <5 y, 5-17 y, and <18 y). We applied this proportion to global estimates of acute lower respiratory infection hospitalizations among children aged <1 y and <5 y, to obtain the number and per capita rate of influenza-associated hospitalizations by geographic region and socio-economic status. Influenza was associated with 10% (95% CI 8%-11%) of respiratory hospitalizations in children <18 y worldwide, ranging from 5% (95% CI 3%-7%) among children <6 mo to 16% (95% CI 14%-20%) among children 5-17 y. On average, we estimated that influenza results in approximately 374,000 (95% CI 264,000 to 539,000) hospitalizations in children <1 y-of which 228,000 (95% CI 150,000 to 344,000) occur in children <6 mo-and 870,000 (95% CI 610,000 to 1,237,000) hospitalizations in children <5 y annually. Influenza-associated hospitalization rates were more than three times higher in developing countries than in industrialized countries (150/100,000 children/year versus 48/100,000). However, differences in hospitalization practices between settings are an important limitation in interpreting these findings. CONCLUSIONS: Influenza is an important contributor to respiratory hospitalizations among young children worldwide. Increasing influen

Background: Immune thrombocytopenic purpura (ITP) is characterized by reduced platelet count secondary to immune-mediated destruction, this results in an increased bleeding risk. Autoantibodies binding to platelets tag them for premature destruction in the spleen. For this reason, splenectomy is often performed as treatment of chronic forms of disease that are resistant to pharmacological therapy. Methods: We studied 30 patients with ITP and compared them with age-matched controls. Results: We show that B cells of patients with chronic ITP are intrinsically hyperreactive, producing more than normal IgG in vivo and plasma cells in vitro. In normal individuals after splenectomy, a significant depletion of memory B cells is observed, associated with loss of reactivity to CpG oligodeoxynucleotide and consequent inability to form antibody-producing cells. In Enzyme-Linked ImmunoSpot Methods, we compared three splenectomized ITP patients relapsing after surgery, 30 healthy controls, and 37 individuals splenectomized for trauma, spherocytosis, thalassemia, nonhematological tumor, and other diseases.Conclusions:We confirmed that B cells of ITP patients remain hyperreactive in vitro and form high numbers of antibody-producing cells after splenectomy. Thus, chronic ITP may be associated with intrinsic B-cell hyperfunction, leading to the production of antibodies with multiple specificities including that against platelets.

The Penman–Monteith equation has been widely used to estimate the maximum evaporation rate (E) from wet/saturated forest canopies, regardless of canopy cover fraction. Forests are then represented as a big leaf and interception loss considered essentially as a one-dimensional process. With increasing forest sparseness the assumptions behind this big leaf approach become questionable. In sparse forests it might be better to model E and interception loss at the tree level assuming that the individual tree crowns behave as wet bulbs (‘‘wet bulb approach”). In this study, and for five different forest types and climate conditions, interception loss measurements were compared to modelled values (Gash’s interception model) based on estimates of E by the Penman–Monteith and the wet bulb approaches. Results show that the wet bulb approach is a good, and less data demanding, alternative to estimate E when the forest canopy is fully ventilated (very sparse forests with a narrow canopy depth). When the canopy is not fully ventilated, the wet bulb approach requires a reduction of leaf area index to the upper, more ventilated parts of the canopy, needing data on the vertical leaf area distribution, which is seldom-available. In such cases, the Penman–Monteith approach seems preferable. Our data also show that canopy cover does not per se allow us to identify if a forest canopy is fully ventilated or not. New methodologies of sensitivity analyses applied to Gash’s model showed that a correct estimate of E is critical for the proper modelling of interception loss.

A multicentre prospective survey on the use of artificial nutrition (AN) and its complications has been carried out on 1657 hospitalised patients including 7.8% in surgical departments, 7.1% in medical departments and 59.9% in intensive care units. Gastrointestinal diseases and cancer were the most frequent pathologies among patients requiring AN. Parenteral nutrition (PN) alone was employed in 1103 (66.5%) patients. In 267 (16.1%) PN was associated with enteral nutrition (EN). 287 (17.3%) patients were fed by the enteral route alone. The average daily energy intake was 35 kcal/kg for central PN, 26 kcal/kg for peripheral PN and 33 kcal/kg for EN. The mean daily nitrogen intake was 0.19 g/kg for central PN, 0.17 g/kg for peripheral PN and 0.20 g/kg for EN. In 10% of patients PN was electrolyte free and in only 50% all the main electrolytes were added. Trace elements were added in 48% of formulations, mainly as multiple combination. Insulin was added in 61% of PN patients, while albumin was given with PN in 20% of patients. Adverse reactions were observed in 10.9% of PN patients and in 11.5% of EN patients. The most frequent complications occurring in parenterally fed patients were infections of the central venous catheter and metabolic alterations (severe electrolyte abnormalities, liver or renal dysfunction, hypo or persistent hyperglycemia). In enterally fed patients the frequent complications were abdominal distension, cramps and diarrhoea. Adverse reactions were responsible for discontinuation of AN in 2.2% of parenterally supported patients and in 5.7% of enterally fed patients.

The region spanning the tumor necrosis factor (TNF) cluster in the human major histocompatibility complex (MHC) has been implicated in susceptibility to numerous immunopathological and inflammatory diseases. However, strong linkage disequilibrium in the region creates conserved TNF block haplotypes; hampering identification of specific polymorphisms responsible for disease phenotypes. Here we review studies investigating TNF block haplotypes and their links with cytokine production and inflammatory disease risk.Recent work has done much to characterize TNF block haplotypes in different ethnicities and their associations with the larger overall structure of MHC ancestral haplotypes. There have also been studies examining the effects of TNF block haplotypes on levels of inflammatory cytokine production, with others investigating haplotype association with diseases risk. Carriage of specific TNF block haplotypes have been associated with increased risk of Type II Diabetes, Chronic Venous Leg Ulceration and Nucleoside Reverse Transcriptase-associated Sensory Neuropathy, all conditions where the underlying pathogenesis lies in a dysregulated inflammatory response. Despite this, no one haplotype was associated with susceptibility to all these diseases, inferring the TNF block region is involved in the multiple pathways of inflammatory dysregulation leading to disease phenotype.The central MHC on human chromosome 6 spans more than 700 kilobases of DNA and contains in excess of 60 genes, making it one of the most gene-dense regions in the human chromosome. A number of diseases, many of them immunopathological in character, have been associated with variations in MHC genes marked by alleles of the HLA loci. It is thought that whilst there are associations with the HLA class II region of the MHC there are also genes within the central MHC which act in conjunction with the class II genes to mediate susceptibility to these diseases. Some of the genes within the central MHC are

The development of highly integrated and compact micro-satellites and the need for a higher precision of positioning and attitude control in space missions has stimulated the development of micro-propulsion systems. At TU-Delft, investigations are focusing on non-chemical, thermal propulsion. TU-Delft is also collaborating with TNO and UTwente to develop a mini-propulsion system (T3-?ps) to be flown in 2011 as part of the MicroNed Nanosatellite Program. To complement these developments, the TU-Delft is developing test facilities and equipment for on ground testing of such systems. The most critical need is the ability to take thrust and impulse bit measurements with adequate accuracy and resolution. To this purpose, the TU-Delft has developed a thrust stand capable of measuring thrust levels in the range 100-1000mN and minimum impulse bit of 5mNs as well as a smaller stand capable of measuring thrusts ranging from 0.5-50mN and a minimum impulse bit of 0.05mNs. In collaboration with Universidad Politécnica de Madrid, we are currently investigating the development of a thrust stand capable of measuring thrust levels in the range 10-2000?N with an accuracy of ± 2?N and minimum impulse bit of 0.25?Ns. In addition, for thruster testing under rough vacuum conditions, a Heraeus vacutherm oven has been successfully adapted. This paper describes the test facility developments made at the TU-Delft as part of the MicroNed program. We will describe the design objectives, and the design methods used, provide an outline of the thrust stands and the vacuum facility and talk about their development status and how well they perform. Also test results will be presented to show the performances of the various thrust stands and the benefits of testing under rough vacuum conditions., Space Engineering, Aerospace Engineering

The development of highly integrated and compact micro-satellites and the need for a higher precision of positioning and attitude control in space missions has stimulated the development of micro-propulsion systems. At TU-Delft, investigations are focusing on non-chemical, thermal propulsion. TU-Delft is also collaborating with TNO and UTwente to develop a mini-propulsion system (T3-?ps) to be flown in 2011 as part of the MicroNed Nanosatellite Program. To complement these developments, the TU-Delft is developing test facilities and equipment for on ground testing of such systems. The most critical need is the ability to take thrust and impulse bit measurements with adequate accuracy and resolution. To this purpose, the TU-Delft has developed a thrust stand capable of measuring thrust levels in the range 100-1000mN and minimum impulse bit of 5mNs as well as a smaller stand capable of measuring thrusts ranging from 0.5-50mN and a minimum impulse bit of 0.05mNs. In collaboration with Universidad Politécnica de Madrid, we are currently investigating the development of a thrust stand capable of measuring thrust levels in the range 10-2000?N with an accuracy of ± 2?N and minimum impulse bit of 0.25?Ns. In addition, for thruster testing under rough vacuum conditions, a Heraeus vacutherm oven has been successfully adapted. This paper describes the test facility developments made at the TU-Delft as part of the MicroNed program. We will describe the design objectives, and the design methods used, provide an outline of the thrust stands and the vacuum facility and talk about their development status and how well they perform. Also test results will be presented to show the performances of the various thrust stands and the benefits of testing under rough vacuum conditions., Space Engineering, Aerospace Engineering

A new approach is suggested for estimating evaporation of intercepted rainfall from single trees in sparse forests. It is shown that, theoretically, the surface temperature of a wet tree crown will depend on the available energy and windspeed. But for a fully saturated canopy under rainy conditions, surface temperature will approach the wet bulb temperature when available energy tends to zero. This was confirmed experimentally from measurements of the radiation balance, aerodynamic conductance for water vapour and surface temperature on an isolated tree crown. Net radiation over a virtual cylindrical surface, enclosing the tree crown, was monitored by a set of radiometers positioned around that surface. Aerodynamic conductance for the tree crown was derived by scaling up measurements of leaf boundary layer conductance using the heated leaf replica method. Thermocouples were used to measure the average leaf surface temperature. Results showed that a fully wet single tree crown behaves like a wet bulb, allowing evaporation of intercepted rainfall to be estimated by a simple diffusion equation for water vapour, which is not restricted by the assumptions of one-dimensional transfer models usually used at the stand scale. Using this approach, mean evaporation rate from wet, saturated tree crowns was 0.27 or 0.30 mm h−1, when surface temperature was taken equal to the air wet bulb temperature or estimated accounting for the available energy, respectively.

This paper is a review of physically-based rainfall interception modelling. Fifteen models were selected, representing distinct concepts of the interception process. Applications of these models to field data sets published before March 2008 are also analysed. We review the theoretical basis of the different models, and give an overview of the models’ characteristics. The review is designed to help with the decision on which model to apply to a specific data set. The most commonly applied models were found to be the original and sparse Gash models (69 cases) and the original and sparse Rutter models (42 cases). The remaining 11 models have received much less attention, but the contribution of the Mulder model should also be acknowledged. The review reveals the need for more modelling of deciduous forest, for progressively more sparse forest and for forest in regions with intensive storms and the consequent high rainfall rates. The present review also highlights drawbacks of previous model applications. Failure to validate models, the few comparative studies, and lack of consideration given to uncertainties in measurements and parameters are the most outstanding drawbacks. Finally, the uncertainties in model input data are rarely taken into account in rainfall interception modelling.

In a previous study, it was shown that an isolated, fully saturated tree-crown behaves like a wet bulb, allowing evaporation of intercepted rainfall to be estimated by a simple diffusion equation for water vapour. This observation was taken as the basis for a new approach in modelling interception loss from savanna-type woodland, whereby the ecosystem evaporation is derived by scaling up the evaporation from individual trees, rather than by considering a homogeneous forest cover. Interception loss from isolated trees was estimated by combining the aforementioned equation for water vapour flux with Gash's analytical model. A new methodology, which avoids the subjectivity inherent in the Leyton method, was used for estimating the crown storage capacity. Modelling performance was evaluated against data from two Mediterranean savanna-type oak woodlands (montados) in southern Portugal. Interception loss estimates were in good agreement with observations in both sites. The proposed modelling approach is physically based, requires only a limited amount of data and should be suitable for the modelling of interception loss in isolated trees and savanna-type ecosystems.

This paper is a review of physically-based rainfall interception modelling. Fifteen models were selected, representing distinct concepts of the interception process. Applications of these models to field data sets published before March 2008 are also analysed. We review the theoretical basis of the different models, and give an overview of the models’ characteristics. The review is designed to help with the decision on which model to apply to a specific data set. The most commonly applied models were found to be the original and sparse Gash models (69 cases) and the original and sparse Rutter models (42 cases). The remaining 11 models have received much less attention, but the contribution of the Mulder model should also be acknowledged. The review reveals the need for more modelling of deciduous forest, for progressively more sparse forest and for forest in regions with intensive storms and the consequent high rainfall rates. The present review also highlights drawbacks of previous model applications. Failure to validate models, the few comparative studies, and lack of consideration given to uncertainties in measurements and parameters are the most outstanding drawbacks. Finally, the uncertainties in model input data are rarely taken into account in rainfall interception modelling.

A new approach is suggested for estimating evaporation of intercepted rainfall from single trees in sparse forests. It is shown that, theoretically, the surface temperature of a wet tree crown will depend on the available energy and windspeed. But for a fully saturated canopy under rainy conditions, surface temperature will approach the wet bulb temperature when available energy tends to zero. This was confirmed experimentally from measurements of the radiation balance, aerodynamic conductance for water vapour and surface temperature on an isolated tree crown. Net radiation over a virtual cylindrical surface, enclosing the tree crown, was monitored by a set of radiometers positioned around that surface. Aerodynamic conductance for the tree crown was derived by scaling up measurements of leaf boundary layer conductance using the heated leaf replica method. Thermocouples were used to measure the average leaf surface temperature. Results showed that a fully wet single tree crown behaves like a wet bulb, allowing evaporation of intercepted rainfall to be estimated by a simple diffusion equation for water vapour, which is not restricted by the assumptions of one-dimensional transfer models usually used at the stand scale. Using this approach, mean evaporation rate from wet, saturated tree crowns was 0.27 or 0.30 mm h−1, when surface temperature was taken equal to the air wet bulb temperature or estimated accounting for the available energy, respectively.

In a previous study, it was shown that an isolated, fully saturated tree-crown behaves like a wet bulb, allowing evaporation of intercepted rainfall to be estimated by a simple diffusion equation for water vapour. This observation was taken as the basis for a new approach in modelling interception loss from savanna-type woodland, whereby the ecosystem evaporation is derived by scaling up the evaporation from individual trees, rather than by considering a homogeneous forest cover. Interception loss from isolated trees was estimated by combining the aforementioned equation for water vapour flux with Gash's analytical model. A new methodology, which avoids the subjectivity inherent in the Leyton method, was used for estimating the crown storage capacity. Modelling performance was evaluated against data from two Mediterranean savanna-type oak woodlands (montados) in southern Portugal. Interception loss estimates were in good agreement with observations in both sites. The proposed modelling approach is physically based, requires only a limited amount of data and should be suitable for the modelling of interception loss in isolated trees and savanna-type ecosystems.

The region spanning the tumour necrosis factor (TNF) cluster in the human major histocompatibility complex is implicated in susceptibility to immunopathological disease, but ethnic differences and linkage disequilibrium have hampered identification of critical polymorphisms. Here, we investigate Europeans, Asians (Bidayuh, Chinese, Indian, Jehai, Malay, Temuan) and Australian Aborigines to provide a framework for disease-association studies. DNA from 999 unrelated healthy donors was genotyped at 38 loci, primarily in coding and promoter regions over a 60-kb region spanning seven genes near TNF. The PHASE algorithm was used to statistically infer TNF block haplotypes and estimate their frequencies in each population. The TNF block is carried as 31 haplotypes in all populations combined, with < 19 in any single population. Only six haplotypes have a unique tag single nucleotide polymorphism (SNP) valid for all populations, but seven haplotypes could be tagged with individual SNPs in selected populations. Four to eight TNF block haplotypes exist across all ethnicities, and hence must pre-date the divergence of these populations from a common ancestor >160000 years ago. Some haplotypes are unique to isolated populations, but they do not contain unique SNP. Hence, they reflect restricted migration and/or extinction of some families rather than de novo mutation.

Associations between major histocompatibility complex (MHC) ancestral haplotypes (AHs) and immunopathological diseases are traditionally ascribed to human leukocyte antigen (HLA) class I or class II alleles. However, polymorphisms in TNF and nearby genes in the central MHC can influence risk. We have defined TNF block haplotypes in Asian, European and Australian Aboriginal donors and shown conservation of TNF block haplotypes in geographically distinct populations, consistent with a common evolutionary origin. Here we show that most TNF block haplotypes do not align with a single MHC AH and associations often vary with ethnicity. This suggests more recent recombination events between the TNF block and the HLA alleles. © 2009 John Wiley & Sons A/S.

Redistribution of ground-level rainfall and interception loss by an isolated Quercus ilex tree were measured over 2 years in a Mediterranean oak savannah. Stemflow, meteorological variables and sap flow were also monitored. Rainfall at ground level was measured by a set of rain-gauges located in a radial layout centred on the tree trunk and extending beyond the crown limits. Interception loss was computed as the difference between the volume of rainwater that would reach the ground in the absence of the tree and the volume of water that actually fell on the ground sampling area (stemflow included). This procedure provided correct interception loss estimates, irrespective of rainfall inclination. Results have shown a clear non-random spatial distribution of ground-level rainfall, with rainwater concentrations upwind beneath the crown and rain-shadows downwind. Interception loss amounted to 22% of gross rainfall, per unit of crown-projected area. Stand interception loss, per unit of ground area, was only 8% of gross rainfall and 28% of tree evapotranspiration. These values reflect the low crown cover fraction of the stand (0·39) and the specific features of the Mediterranean rainfall regime (predominantly with few large storms). Nevertheless, it still is an important component of the water balance of these Mediterranean ecosystems

Redistribution of ground-level rainfall and interception loss by an isolated Quercus ilex tree were measured over 2 years in a Mediterranean oak savannah. Stemflow, meteorological variables and sap flow were also monitored. Rainfall at ground level was measured by a set of rain-gauges located in a radial layout centred on the tree trunk and extending beyond the crown limits. Interception loss was computed as the difference between the volume of rainwater that would reach the ground in the absence of the tree and the volume of water that actually fell on the ground sampling area (stemflow included). This procedure provided correct interception loss estimates, irrespective of rainfall inclination. Results have shown a clear non-random spatial distribution of ground-level rainfall, with rainwater concentrations upwind beneath the crown and rain-shadows downwind. Interception loss amounted to 22% of gross rainfall, per unit of crown-projected area. Stand interception loss, per unit of ground area, was only 8% of gross rainfall and 28% of tree evapotranspiration. These values reflect the low crown cover fraction of the stand (0·39) and the specific features of the Mediterranean rainfall regime (predominantly with few large storms). Nevertheless, it still is an important component of the water balance of these Mediterranean ecosystems

A method for analysing the degree of visual interference from a sensitive area (an area from which a view of the quarry is not desirable) is described. There are three types of problems: reclaiming derelict quarries; defining impact attenuation principles in operating quarries; and selecting sites for new quarries. The analysis is applied to the Colleferro Valley, central Italy., A method for analysing the degree of visual interference from a sensitive area (an area from which a view of the quarry is not desirable) is described. There are three types of problems: reclaiming derelict quarries; defining impact attenuation principles in operating quarries; and selecting sites for new quarries. The analysis is applied to the Colleferro Valley, central Italy.

Supersedes AD-153 530.