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3 results on '"Wauben MH"'

1. Updating the MISEV minimal requirements for extracellular vesicle studies: building bridges to reproducibility

Author

Witwer, KW, Soekmadji, C, Hill, AF, Wauben, MH, Buzas, EI, Di Vizio, D, Falcon-Perez, JM, Gardiner, C, Hochberg, F, Kurochkin, IV, Lotvall, J, Mathivanan, S, Nieuwland, R, Sahoo, S, Tahara, H, Torrecilhas, AC, Weaver, AM, Yin, H, Zheng, L, Gho, YS, Quesenberry, P, Thery, C, Witwer, KW, Soekmadji, C, Hill, AF, Wauben, MH, Buzas, EI, Di Vizio, D, Falcon-Perez, JM, Gardiner, C, Hochberg, F, Kurochkin, IV, Lotvall, J, Mathivanan, S, Nieuwland, R, Sahoo, S, Tahara, H, Torrecilhas, AC, Weaver, AM, Yin, H, Zheng, L, Gho, YS, Quesenberry, P, and Thery, C

Published
2017

2. Minimal experimental requirements for definition of extracellular vesicles and their functions: a position statement from the International Society for Extracellular Vesicles.

Author

Lötvall, J, Hill, AF, Hochberg, F, Buzás, EI, Di Vizio, D, Gardiner, C, Gho, YS, Kurochkin, IV, Mathivanan, S, Quesenberry, P, Sahoo, S, Tahara, H, Wauben, MH, Witwer, KW, Théry, C, Lötvall, J, Hill, AF, Hochberg, F, Buzás, EI, Di Vizio, D, Gardiner, C, Gho, YS, Kurochkin, IV, Mathivanan, S, Quesenberry, P, Sahoo, S, Tahara, H, Wauben, MH, Witwer, KW, and Théry, C

Abstract

Secreted membrane-enclosed vesicles, collectively called extracellular vesicles (EVs), which include exosomes, ectosomes, microvesicles, microparticles, apoptotic bodies and other EV subsets, encompass a very rapidly growing scientific field in biology and medicine. Importantly, it is currently technically challenging to obtain a totally pure EV fraction free from non-vesicular components for functional studies, and therefore there is a need to establish guidelines for analyses of these vesicles and reporting of scientific studies on EV biology. Here, the International Society for Extracellular Vesicles (ISEV) provides researchers with a minimal set of biochemical, biophysical and functional standards that should be used to attribute any specific biological cargo or functions to EVs.

Published
2014

3. Minimal experimental requirements for definition of extracellular vesicles and their functions: a position statement from the International Society for Extracellular Vesicles.

Author

Lötvall, J, Hill, AF, Hochberg, F, Buzás, EI, Di Vizio, D, Gardiner, C, Gho, YS, Kurochkin, IV, Mathivanan, S, Quesenberry, P, Sahoo, S, Tahara, H, Wauben, MH, Witwer, KW, Théry, C, Lötvall, J, Hill, AF, Hochberg, F, Buzás, EI, Di Vizio, D, Gardiner, C, Gho, YS, Kurochkin, IV, Mathivanan, S, Quesenberry, P, Sahoo, S, Tahara, H, Wauben, MH, Witwer, KW, and Théry, C

Abstract

Secreted membrane-enclosed vesicles, collectively called extracellular vesicles (EVs), which include exosomes, ectosomes, microvesicles, microparticles, apoptotic bodies and other EV subsets, encompass a very rapidly growing scientific field in biology and medicine. Importantly, it is currently technically challenging to obtain a totally pure EV fraction free from non-vesicular components for functional studies, and therefore there is a need to establish guidelines for analyses of these vesicles and reporting of scientific studies on EV biology. Here, the International Society for Extracellular Vesicles (ISEV) provides researchers with a minimal set of biochemical, biophysical and functional standards that should be used to attribute any specific biological cargo or functions to EVs.

Published
2014

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