1. A novel cetacean adenovirus in stranded harbour porpoises from the North Sea: detection and molecular characterization
- Author
-
van Beurden, Steven J, IJsseldijk, Lonneke L, van de Bildt, Marco W G, Begeman, Lineke, Wellehan, James F X, Waltzek, Thomas B, de Vrieze, Geert, Gröne, Andrea, Kuiken, Thijs, Verheije, M Hélène, Penzes, Judit J, van Beurden, Steven J, IJsseldijk, Lonneke L, van de Bildt, Marco W G, Begeman, Lineke, Wellehan, James F X, Waltzek, Thomas B, de Vrieze, Geert, Gröne, Andrea, Kuiken, Thijs, Verheije, M Hélène, and Penzes, Judit J
- Abstract
Harbour porpoises (Phocoena phocoena) are the most prevalent cetaceans in the North Sea. The fecal viral flora of 21 harbour porpoises stranded along the Dutch coastline was analyzed by a metagenomics approach. Sequences of a novel cetacean mastadenovirus, designated harbour porpoise adenovirus 1 (HpAdV-1), were detected. The sequence of a 23-kbp genomic region, spanning the conserved late region, was determined using primer walking. Phylogenetic analysis indicated that HpAdV-1 is most closely related to bottlenose dolphin adenovirus and clusters with Cetartiodactyla adenoviruses. The prevalence of HpAdV-1 was low (2.6%) based on targeted PCR-screening of the intestinal contents of 151 harbour porpoises stranded between 2010 and 2013.
- Published
- 2017