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[ES] Este trabajo se centra en el uso de biomasa como combustible en una celda de combustible de óxido sólido. Se puede separar en dos partes. La primera es una parte teórica que explica qué es una celda de combustible, cómo funcionan y los diferentes tipos y aplicaciones que tienen. La segunda parte es una parte experimental en la que se realiza el experimento para probar la biomasa como combustible para la pila de combustible y se muestran los resultados., [EN] This work is focus on the use of biomass as a fuel in a solid oxide fuel cell. It can be separated in two parts. The first one is a theorical part that explains what a fuel cell is, how they operate and the different types and applications that they have. The second part is an experimental part in which is explained the experiment done to prove biomass as a fuel for the fuel cell and are shown the results.

[ES] Este trabajo se centra en el uso de biomasa como combustible en una celda de combustible de óxido sólido. Se puede separar en dos partes. La primera es una parte teórica que explica qué es una celda de combustible, cómo funcionan y los diferentes tipos y aplicaciones que tienen. La segunda parte es una parte experimental en la que se realiza el experimento para probar la biomasa como combustible para la pila de combustible y se muestran los resultados., [EN] This work is focus on the use of biomass as a fuel in a solid oxide fuel cell. It can be separated in two parts. The first one is a theorical part that explains what a fuel cell is, how they operate and the different types and applications that they have. The second part is an experimental part in which is explained the experiment done to prove biomass as a fuel for the fuel cell and are shown the results.

[ES] Este trabajo se centra en el uso de biomasa como combustible en una celda de combustible de óxido sólido. Se puede separar en dos partes. La primera es una parte teórica que explica qué es una celda de combustible, cómo funcionan y los diferentes tipos y aplicaciones que tienen. La segunda parte es una parte experimental en la que se realiza el experimento para probar la biomasa como combustible para la pila de combustible y se muestran los resultados., [EN] This work is focus on the use of biomass as a fuel in a solid oxide fuel cell. It can be separated in two parts. The first one is a theorical part that explains what a fuel cell is, how they operate and the different types and applications that they have. The second part is an experimental part in which is explained the experiment done to prove biomass as a fuel for the fuel cell and are shown the results.

Consumption of dietary bioactives is an avenue to enhancing the effective healthiness of diets by attenuating the glycaemic response. The intestinal brush border enzyme sucrase-isomaltase (SI) is the sole enzyme hydrolysing consumed sucrose, and we previously showed the acute effects of olive leaf extract (OLE) on sucrase activity when given together with sugars both in vitro and in vivo. Here we tested whether OLE could affect sucrase expression when pre-incubated chronically, a "priming" effect not dependent on competitive interaction with SI, in both a cell model and a human intervention. Using differentiated Caco-2/TC7 cells, long-term pre-treatment with oleuropein-rich olive leaf extract (OLE) lowered SI mRNA, surface protein and activity, and attenuated subsequent sucrose hydrolysis. Based on these results, a randomised, double-blinded, placebo-controlled, crossover pilot study was conducted. OLE (50 mg oleuropein) was consumed in capsule form 3 times a day for 1 week by 11 healthy young women followed by an oral sucrose tolerance test in the absence of OLE. However this treatment, compared to placebo, did not induce a change in post-prandial blood glucose maximum concentration (Glcmax), time to reach Glcmax and incremental area under the curve. These results indicate that changes in SI mRNA, protein and activity in an intestinal cell model by OLE are not sufficient under these conditions to induce a functional effect in vivo in healthy volunteers.Copyright © 2019 by the authors. Licensee MDPI, Basel, Switzerland.

Consumption of dietary bioactives is an avenue to enhancing the effective healthiness of diets by attenuating the glycaemic response. The intestinal brush border enzyme sucrase-isomaltase (SI) is the sole enzyme hydrolysing consumed sucrose, and we previously showed the acute effects of olive leaf extract (OLE) on sucrase activity when given together with sugars both in vitro and in vivo. Here we tested whether OLE could affect sucrase expression when pre-incubated chronically, a "priming" effect not dependent on competitive interaction with SI, in both a cell model and a human intervention. Using differentiated Caco-2/TC7 cells, long-term pre-treatment with oleuropein-rich olive leaf extract (OLE) lowered SI mRNA, surface protein and activity, and attenuated subsequent sucrose hydrolysis. Based on these results, a randomised, double-blinded, placebo-controlled, crossover pilot study was conducted. OLE (50 mg oleuropein) was consumed in capsule form 3 times a day for 1 week by 11 healthy young women followed by an oral sucrose tolerance test in the absence of OLE. However this treatment, compared to placebo, did not induce a change in post-prandial blood glucose maximum concentration (Glcmax), time to reach Glcmax and incremental area under the curve. These results indicate that changes in SI mRNA, protein and activity in an intestinal cell model by OLE are not sufficient under these conditions to induce a functional effect in vivo in healthy volunteers.Copyright © 2019 by the authors. Licensee MDPI, Basel, Switzerland.

The present paper demonstrates the topicality and effectiveness of using the gas-liquid chromatography method in a forensic-medical examination for an analysis of the content of alcohol, narcotic and toxic substances in biological samples., В данной работе показана актуальность и эффективность использования метода газожидкостной хроматографии в судебно-медицинской экспертизе для анализа содержания алкоголя, ядовитых и наркотических веществ в биологических образцах., У даній роботі показана актуальність і ефективність використання методу газорідинної хроматографії в судово-медичній експертизі для аналізу вмісту алкоголю, отруйних і наркотичних речовин у біологічних зразках.