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The current study describes the chemical composition, antifungal, antibiofilm, antibacterial and molecular docking studies of Syzygium dyerianumgrowing in Malaysia. The essential oil was obtained through hydrodistillation and characterized using gas chromatography (GC-FID) and gas chromatography-mass spectrometry (GC-MS). The antifungal and antibacterial activities were developed using the broth microdilution assay, whereas the effect on the microbial biofilms was determined using a semi-quantitative static biofilm assay. A total of 31 components were identified, which represent 99.5 % of the essential oil. The results revealed that the essential oil consisted mainly of β-pinene (15.6 %), α-terpineol (13.3 %), α-pinene (11.1 %), caryophyllene oxide (8.8 %), limonene (8.1 %), borneol (6.0 %) and viridiflorol (5.1 %). The results of the microdilution method showed that essential oil exhibited activity against Candida albicansand Streptococcus mutanswith minimal inhibitory concentration values of 125 and 250 μg/mL, respectively. Furthermore, essential oil decreased the biofilm of C. albicansand S. mutansby 20.11 ± 0.27 % and 32.10 ± 4.81 % when treated with 250 μg/mL. The best docking energy was observed with viridiflorol (−29.7 kJ/mol). This study highlights that essential oil can potentially be a natural antifungal, antibacterial, and antibiofilm agent that could be applied in the pharmaceutical and food industries.

The combination of S. salivarius K12 and M. acuminata are being used as synbiotic, but its safety evaulation is required.