Search

High rates of youth unemployment across the world have captured the attention of many world organizations and other policy makers. One policy solution that has been proposed to curb these high rates is encouraging youth entrepreneurship. In this paper, we examine the formation of attitudes that are favorable to entrepreneurship using data from 185 business students in South India. We adopt an approach that tests the relative efficacy of two principal factors in the formation of entrepreneurial attitude, i.e., stocks of youth human/social capital and a series of personality traits. Results from a probit model suggest that the youth’s prior labor market experience, the social capital that youth have accumulated through volunteering, and the social connections that parents have made are all highly predictive of pro-entrepreneurial attitudes; personality traits exert less importance. Implications for these findings are discussed for the creation of strategies that can stimulate entrepreneurship among youth as one way to combat high rates of youth unemployment.

ABSTRACTThe aim of the present study was to synthesize silver (Ag) and titanium dioxide (TiO2) nanoparticles (NPs) using green synthesis from aqueous leaf extract of Euphorbia prostrataas antileishmanial agents and to explore the underlying molecular mechanism of induced cell death. In vitroantileishmanial activity of synthesized NPs was tested against promastigotes of Leishmania donovaniby alamarBlue and propidium iodide uptake assays. Antileishmanial activity of synthesized NPs on intracellular amastigotes was assessed by Giemsa staining. The leishmanicidal effect of synthesized Ag NPs was further confirmed by DNA fragmentation assay and by cell cycle progression and transmission electron microscopy (TEM) of the treated parasites. TEM analysis of the synthesized Ag NPs showed a spherical shape with an average size of 12.82 ± 2.50 nm, and in comparison to synthesized TiO2NPs, synthesized Ag NPs were found to be most active against Leishmaniaparasites after 24 h exposure, with 50% inhibitory concentrations (IC50) of 14.94 μg/ml and 3.89 μg/ml in promastigotes and intracellular amastigotes, respectively. A significant increase in G0/G1phase of the cell cycle with a subsequent decrease in S (synthesis) and G2/M phases compared to controls was observed. The growth-inhibitory effect of synthesized Ag NPs was attributed to increased length of S phase. A decreased reactive oxygen species level was also observed, which could be responsible for the caspase-independent shift from apoptosis (G0/G1arrest) to massive necrosis. High-molecular-weight DNA fragmentation as a positive consequence of necrotic cell death was also visualized. We also report that the unique trypanothione/trypanothione reductase (TR) system of Leishmaniacells was significantly inhibited by synthesized Ag NPs. The green-synthesized Ag NPs may provide promising leads for the development of cost-effective and safer alternative treatment against visceral leishmaniasis.