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We characterized partial sequences of 18S rDNA from sedge warblers infected with a parasite described previously as Hepatozoon kabeeni. Prevalence was 47% in sampled birds. We detected 3 parasite haplotypes in 62 sequenced samples from infected animals. In phylogenetic analyses, 2 of the putative Hepatozoon haplotypes closely resembled Lankesterella minima and L. valsainensis. The third haplotype grouped in a wider clade composed of Caryospora and Eimeria. None of the haplotypes showed resemblance to sequences of Hepatozoon from reptiles and mammals. Molecular detection results were consistent with those from microscopy of stained blood smears, confirming that the primers indeed amplified the parasite sequences. Here we provide evidence that the avian Hepatozoon-like parasites are most likely Lankesterella, supporting the suggestion that the systematic position of avian Hepatozoon-like species needs to be revised. [ABSTRACT FROM AUTHOR]

Studies on nesting preferences of common buzzard and goshawk were carried out in two distinctly different parts of the Niepolomice Forest (S Poland): deciduous (oak-hornbeam wood) and coniferous. Characteristics of nest sites were determined on three spatial scales, separately for: (1) nest tree; (2) nest tree area (0.07 ha circle centred at nest tree) and (3) nest stand (15 ha circle centred at nest tree). Nesting preferences discovered for the nest tree and its surroundings, included height and diameter of trees, age of the forest stand, distance to the nearest open area or forest road and occurrence of open areas in the vicinity of the nest. In the diverse habitat of oak-hornbeam wood, more similar to natural woods, nest site selection operated on several levels, possibly starting at the most extensive end of the scale before narrowing to the selection of a particular nest tree. In the more homogeneous habitat of commercially exploited coniferous forest, the surrounding of the nest were found to be insignificant, and the nesting decisions were likely to be based principally on individual characteristics of a tree i.e. its shape and size, being suitable for nesting.