1. Development of a TILLING resource in durum wheat for reverse- and forward-genetic analyses
- Author
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Bovina, R., Brunazzi, A., Gasparini, G., Sestili, F., Palombieri, S., Botticella, E., Lafiandra, D., Mantovani, P., and Massi, A.
- Abstract
A durum wheat TILLING (targeting induced local lesions in genomes) population of 2601M3 families was developed from cv. Svevo using ethyl methanesulfonate as a chemical mutagen. The entire M3 population was field-grown for phenotypic evaluations. Despite the polyploid nature of the wheat genome, a preliminarily phenotypic screening showed a high frequency of morphological alterations (~22%); specific phenotyping for seed morphology was undertaken. Furthermore, a reverse-genetics experiment was performed on DNA collected from M2 leaves for the homoeologous genes SBEIIa-A and SBEIIa-B involved in starch metabolism. One non-sense mutation for both genes was identified; specific crosses are planned in order to pyramid the two mutations.
- Published
- 2014
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