High‐resolution nuclear magnetic resonance (NMR) spectroscopy is an indispensable technique for obtaining chemical structure information. Its quantitative and noninvasive properties have led to its growing popularity as an analytical tool in many fields, including biology, chemistry, medicine, and food science. During transportation and storage, chemical reactions among the many nutrients lead to a loss of food quality. In these circumstances, portable NMR spectrometers can readily be used for food inspection and quality control. Because of the heterogeneous tissue distribution in food, a high‐resolution NMR method is required for detailed food inspection. Therefore, in this study, we demonstrated the feasibility of using an intermolecular double‐quantum coherence signal to obtain high‐resolution metabolic profiles of several fruits, including grape, cantaloupe, tomato, and watermelon. The resulting high‐resolution NMR spectra facilitate the identification of important metabolites, which can be used as biomarkers for food quality control. The method established here may be adapted for food inspection using portable NMR equipment. 1H nuclear magnetic resonance spectra of tomato tissue by using single pulse sequence (a) and intermolecular double quantum coherence sequence (b). Tomato tissue was transferred into the tube directly without pretreatment.