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The aim of this study was to characterise the genomic and phenotypic characteristics of a colistin-resistant Klebsiella pneumoniaeisolate causing a lethal infection that was phenotypically resistant to carbapenems and colistin.

Firstly, the suitable forming method of graphite ring was screened according to compression deformation test, considering the characteristics of radial clearance seal. Then, the compression resilience, radial contact stress and axial sealing performance of graphite packing rings with different density were researched by testing. Research shows: 25% compressibility is the limit of radial clearance compensation ability of graphite packing ring; And the radial contact stress of graphite packing ring on the pipe wall is linearly positively correlated with the axial load, density has little effect on it. Based on the porous media model of gaskets, axial leakage models of three kinds of graphite rings with different density were obtained by experimental fitting and the influence of external working conditions on leakage mode was analyzed, which provide a theoretical basis for the design of graphite ring seal based on leakage rate.