Gyulavári, HajnalkaAnna, Felföldi, Tamás, Benken, Theodor, Szabó, LászlóJózsef, Miskolczi, Margit, Cserháti, Csaba, Horvai, Valér, Márialigeti, Károly, and Dévai, György
Morphometric and genetic differences were analysed for two closely related damselflies, Chalcolestes viridisand C. parvidens. A total of 305 male individuals were collected from six European countries (Austria, Croatia, Germany, Greece, Hungary and Portugal). Measurements from a total of 28 populations of C. viridisand C. parvidensand several intermediate forms were collected to determine if they can be definitely distinguished using simple morphometric characters. DNA sequences from two independent loci (nuclear ribosomal ITS region and mitochondrial cytochrome oxidase I gene) were analysed to test whether these taxa represent separate monophyletic groups as well as to compare the genetic distance with those found between well-accepted European Lestesspecies. Discriminant analysis revealed that C. viridisand C. parvidensare differentiated in morphometric space. Individuals with intermediate anal appendage traits overlapped with both C. viridisand C. parvidenswhich raised the possibility that they are merely subspecies of a single species. However, genetic analysis of both investigated DNA regions showed that the two Chalcolestestaxa did not share haplotypes, indicating their status as true species. Furthermore, they formed a monophyletic group separated from the investigated Lestesspecies, supporting the recognition of the genus Chalcolestes. The two Chalcolestesspecies are very closely related compared with European Lestesspecies, suggesting that their divergence occurred relatively recently.