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Seed weight is a component of seed yield in rapeseed (Brassica napusL.). Although quantitative trait loci (QTL) for seed weight have been reported in rapeseed, only a few causal quantitative trait genes (QTGs) have been identified, resulting in a limitation in understanding of seed weight regulation. We constructed a gene coexpression network at the early seed developmental stage using transcripts of 20,408 genes in QTL intervals and 1017 rapeseed homologs of known genes from other species. Among the 10 modules in this gene coexpression network, modules 1 and 2 were core modules and contained genes involved in source–flow–sink processes such as synthesis and transportation of fatty acid and protein, and photosynthesis. A hub gene SERINE CARBOXYPEPTIDASE-LIKE 19(SCPL19) was identified by candidate gene association analysis in rapeseed and functionally investigated using ArabidopsisT-DNA mutant and overexpression lines. Our study demonstrates the power of gene coexpression analysis to prioritize candidate genes from large candidate QTG sets and enhances the understanding of molecular mechanism for seed weight at the early developmental stage in rapeseed.

Seed number per silique (SNPS) is one of seed yield components in rapeseed, but its genetic mechanism remains elusive. Here a double haploid (DH) population derived from a hybrid between female 6Q006 with 35–40 SNPS and male 6W26 with 10–15 SNPS was investigated for SNPS in the year 2017, 2018, 2019 and 2021, and genotyped with Brassica60K Illumina Infinium SNP array. An overlapping major QTL (qSNPS.C09) explaining 51.50% of phenotypic variance on average was narrowed to a 0.90 Mb region from 44.87 Mb to 45.77 Mb on chromosome C09 by BSA-seq. Subsequently, two DEGs in this interval were detected between extreme individuals in DH and F2populations by transcriptome sequencing at 7 and 14 days after pollination siliques. Of which, BnaC09g45400Dencoded an adenine phosphoribosyltransferase 5 (APT5) has a 48-bp InDel variation in the promoter of two parents. Candidate gene association analysis showed that this InDel variation was associated with SNPS in a nature population of rapeseed, where 54 accessions carrying the same haplotype as parent 6Q006 had higher SNPS than 103 accessions carrying the same haplotype as parent 6W26. Collectively, the findings are helpful for rapeseed molecular breeding of SNPS, and provide new insight into the genetic and molecular mechanism of SNPS in rapeseed.

Isotopically labeled amino acids are widely used to study the structure and dynamics of proteins by NMR. Herein we describe a facile, gram-scale synthesis of compounds 1band 2bunder standard laboratory conditions from the common intermediate 7. 2bis obtained via simple deprotection, while 1bis accessed through a reductive deoxygenation/deuteration sequence and deprotection. 1band 2bprovide improved signal intensity using lower amounts of labeled precursor and are alternatives to existing labeling approaches.

Tuberculosis (TB) is the leading cause of global morbidity and mortality resulting from infectious disease, with over 10.6 million new cases and 1.4 million deaths in 2021. This global emergency is exacerbated by the emergence of multidrug-resistant MDR-TB and extensively drug-resistant XDR-TB; therefore, new drugs and new drug targets are urgently required. From a whole cell phenotypic screen, a series of azetidines derivatives termed BGAz, which elicit potent bactericidal activity with MIC99values <10 μM against drug-sensitive Mycobacterium tuberculosisand MDR-TB, were identified. These compounds demonstrate no detectable drug resistance. The mode of action and target deconvolution studies suggest that these compounds inhibit mycobacterial growth by interfering with cell envelope biogenesis, specifically late-stage mycolic acid biosynthesis. Transcriptomic analysis demonstrates that the BGAz compounds tested display a mode of action distinct from the existing mycobacterial cell wall inhibitors. In addition, the compounds tested exhibit toxicological and PK/PD profiles that pave the way for their development as antitubercular chemotherapies.

Previous research has shown that children and adults express more confidence in the existence of unobservable scientific (e.g., germs), as compared to religious, phenomena (e.g., the soul). We asked if the same pattern would emerge among parents (N= 77) and children (N= 85) in Iran, a country with widespread commitment to religious values and beliefs. Parents expressed confidence in the existence of various scientific and religious phenomena but were more confident that scientific phenomena exist. Moreover, even though Iranian parents expressed a high valuation of both science and religion, their valuation of science was higher than that of religion. Like their parents, older children also expressed greater confidence in the existence of scientific, as compared to religious, phenomena. We conclude that with age, the kind of discourse children are exposed to elicits more confidence in the existence of scientific phenomena even in a religious society.

Whereas a large body of research has focused on the development of children as learners, relatively little research has focused on the development of children as teachers. Moreover, even less research has focused on the potential cognitive mechanisms associated with high-quality teaching. Here, we review evidence that children’s selective teaching is associated with at least three cognitive skills: the ability to represent mental states, the ability to infer mental states in real-time (i.e., what a pupil knows based on his or her behavior), as well as executive function skills. We note potential cultural differences in children’s teaching and highlight the need for future research.