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A new superconducting magnet test facility was created at the MIT Plasma Science and Fusion Center (PSFC) for the SPARC Toroidal Field Model Coil (TFMC) program. The facility was designed and constructed in parallel with the TFMC between 2019 and 2021, with capabilities and design approaches tailored to the needs of this project and its time line. The major components of the facility include a new cryostat (outer dimensions, 5.3 m × 3.7 m × 1.5 m) with open bore; a novel cooling system circulating supercritical helium in a closed loop to provide $\sim$600 W cooling power at $\sim$20 bar-a, $\sim$20 K; a 50-kA $\pm$ 10-V power supply with supporting nitrogen-cooled high temperature superconductor (HTS) binary current leads operating at record currents, as well as VIPER-cable HTS cold bus; and a new instrumentation and programmable-logic-controller-based control system handling $\sim$650 input and output signals distributed between the facility and the test article. Substantial legacy infrastructure inherited from the PSFC's Alcator C-Mod tokamak program, including liquid nitrogen facilities and 10 mW of ac power, was instrumental in the rapid deployment of these new systems. Immediately after initial commissioning, the facility was used successfully to test the SPARC TFMC, operating the magnet in a campaign achieving 20 T on the coil, as well as a second campaign performing quench testing. The facility has since undergone several upgrades and has been used in campaigns of other test articles, and it is expected that the facility will remain a resource for the community for the foreseeable future to develop fusion magnets and related technology.

In this article, the SPARC Toroidal Field Model Coil (TFMC) experimental tests are described. The tests include detailed comparisons to a hierarchy of electromagnetic and structural models of the coil. The tests confirm the ability of the no-insulation no-twist (NINT) configuration to provide highly stable dc operations with peak magnetic field in excess of 20 T at the rare earth yttrium barium copper oxide (REBCO) tape stacks. The advantages of the modular TFMC approach are validated including the ability to test and probe the REBCO and structural response of the coil in stages, a strategy that will be applied to large-scale coil production for SPARC. The electromagnetic models show high fidelity agreement to the dc and charging coil performance in terms of current distribution, voltages, and heating. The transient response of the TFMC to open-circuit events is examined. The coil exhibits the positive features of the NINT design with very low induced voltages and a predictable redistribution of current and resulting volumetric heating. However, highly localized damage occurs during a programmed open circuit at operational conditions similar to those used in the SPARC tokamak, in agreement with calibrated electromagnetic models. This reveals the cause and remedy to the thermal instability that gives rise to the damage. Therefore, the conclusion of the tests is that the NINT TFMC is confirmed as meeting the programmatic requirements for SPARC and, more generally, for high-field tokamaks.

AbstractIn oviparous reptiles with no parental care, the choice of nest site is a mother's final investment in her offspring. Although linkages between nest site choice, egg temperatures, and embryonic success have been well studied, much less is known about analogous linkages with soil moisture encompassing developing embryos. Most ground-nesting reptiles nest at depths <25 cm, with the deepest nests <1.0 m deep. Recently, however, the nests of two species of monitor lizards (Varanus panoptesand V. gouldii) have been discovered at depths of 2.3–3.0 m, suggesting that nesting at extreme depths in these species is an adaptive response to the lack of sufficient soil moisture at shallower depths. Herein, we examine this idea with V. panoptes, specifically predicting that deeper nests in a desert ecosystem compared with those in a savannah ecosystem are attributable to differences in the magnitude of rainfall. We excavated a communal nesting warren to a depth of 4 m and identified 11 fresh nests and 99 hatched nests. Mean nest depth in the present study was greater than that in savannah. However, nests were shallower than those of V. gouldiiin the same general location, possibly because of local heterogeneity in soil moisture. Hatchlings excavated their own emergence burrows rather than following the burrows of their mothers, despite relatively great distances through resistant soils. Collectively, deep nesting creates energetic challenges for mothers and hatchlings, suggesting an adaptive function for the behavior.

AbstractA 5-GHz steady-state lower hybrid (LH) current drive (LHCD) system is planned to support steady-state and advanced tokamak operation on the Korea Superconducting Tokamak Advanced Research (KSTAR) experiment. As an initial phase, a pulsed 5-GHz, 500-kW LHCD system has been installed in KSTAR for basic experimental studies of the LH coupling and flux saving in the plasma current ramp-up, prior to long-pulse noninductive operation in KSTAR. A Toshiba-made klystron developed in collaboration with Pohang University of Science and Technology in 2006 is utilized for the initial KSTAR LHCD system. The LH launcher is designed as a fully active waveguide grill type with a parallel refractive index n[parallel]value ranging from 1.8 to 4.3 and with high directivity. In the initial stage, the LH launcher consists of eight columns of four-way power splitters and two columns of dummy waveguides, one on each side. The operational n[parallel]value is fixed at 2.1 but can be adjusted by replacing waveguide components external to the vacuum vessel. Since the target operation pulse duration of the initial LHCD system is 2 s with an output power of 500 kW at the klystron window, the prototype klystron was recently successfully conditioned to a radio frequency power of 514 kW for a maximum pulse duration of 3 s using a matched dummy load (voltage standing wave ratio of 1.16:1). This paper presents the progress of the initial KSTAR LHCD system and the performance test results of the prototype klystron. The research plan aiming at steady-state LHCD operation in KSTAR is also described in this paper.

We have developed high power four and eight way splitters for a new Lower Hybrid launcher. The motivation for the new launcher was the need to provide more power and reliability to the launcher structure. In addition there was a desire to simplify and increase the reliability of the implementation of the alumina windows. The launcher consists of 64 waveguide apertures powered by 8 klystrons with maximum power of 250 kW each at 4.6 GHz. Hence, it is necessary to split the power from each Klystron into eight separate waveguides. The outputs of the splitter have a difference in power less than 0.1dB and phase less than 2 degree. The design analysis of the splitter was done with the computer code CST. Structure analysis was performed using Ansys. The splitter is fabricated by machining an open cavity into a thick stainless steel plate creating the specified internal geometry. It is machined to a tight tolerance of +/- 0.005″. A fitted lid is then welded on top of the open cavity using electron beam welding. The excess metal is removed with Electro discharge machining (EDM) creating the external geometry. The waveguides are then butt-welded to the splitter. Welding fixtures/parameters are being developed to achieve the desired tolerances. Two methods for attaching the ceramic windows are being evaluated, brazing and electro-forming.

The reproductive biology of female pig-nosed turtles Carettochelys insculpta was studied for 4 years in the wet–dry tropics of northern Australia. Females matured at around 6 kg body mass (38.0 cm curved carapace length, 30.5 cm plastron length). Turtles produced egg sizes and clutch sizes similar to that of other turtle species similar in size. Turtles reproduced every second year, but produced two clutches within years, about 41 days apart. Thus, it appeared that females were energy limited, possibly due to the low available energy content of the dry season diet (aquatic vegetation). Life-history theory predicts that some costly behaviour associated with reproduction exists, such that by skipping years turtles could reduce that cost and put the savings into future reproduction. Previous work revealed no behaviour associated with reproduction in the population. Within years, clutch mass did not differ between early (first) and late (second) clutches. However, early clutches tended to have more eggs per clutch but smaller eggs than late clutches, a new finding for turtles that has been demonstrated in lizards and other animals. Because the study spanned both years with ‘big’ and ‘small’ wet seasons, we were able to examine how the magnitude of the wet season influenced reproductive characteristics. Following big wet seasons, turtles produced larger, heavier, and more eggs per clutch than they did after small wet seasons. Relationships among body size, egg size and clutch size were evident after two big wet seasons but not apparent after two small wet seasons. Collectively, annual variation in reproductive characteristics and current life-history theory suggest that a big wet season is a time of high energy accumulation for the turtles.

TGFbeta can override the proliferative effects of EGF and other Ras-activating mitogens in normal epithelial cells. However, epithelial cells harboring oncogenic Ras mutations often show a loss of TGFbeta antimitogenic responses. Here we report that oncogenic Ras inhibits TGFbeta signaling in mammary and lung epithelial cells by negatively regulating the TGFbeta mediators Smad2 and Smad3. Oncogenically activated Ras inhibits the TGFbeta-induced nuclear accumulation of Smad2 and Smad3 and Smad-dependent transcription. Ras acting via Erk MAP kinases causes phosphorylation of Smad2 and Smad3 at specific sites in the region linking the DNA-binding domain and the transcriptional activation domain. These sites are separate from the TGFbeta receptor phosphorylation sites that activate Smad nuclear translocation. Mutation of these MAP kinase sites in Smad3 yields a Ras-resistant form that can rescue the growth inhibitory response to TGFbeta in Ras-transformed cells. EGF, which is weaker than oncogenic mutations at activating Ras, induces a less extensive phosphorylation and cytoplasmic retention of Smad2 and Smad3. Our results suggest a mechanism for the counterbalanced regulation of Smad2/Smad3 by TGFbeta and Ras signals in normal cells, and for the silencing of antimitogenic TGFbeta functions by hyperactive Ras in cancer cells.

Transforming growth factor‐beta (TGF‐beta) signals by contacting two distantly related transmembrane serine/threonine kinases called receptors I (T beta R‐I) and II (T beta R‐II). TGF‐beta binds to T beta R‐II, which is a constitutively active kinase and this complex recruits T beta R‐I, causing its phosphorylation and signal propagation to downstream substrates. The biochemical properties of this interaction were analyzed with reconstituted receptor systems. T beta R‐I and T beta R‐II baculovirally expressed at high levels in insect cells have the ligand binding properties of receptors expressed in mammalian cells, and form a complex in which T beta R‐I phosphorylation is dependent on the kinase activity of T beta R‐II. Furthermore, T beta R‐I and T beta R‐II can form a complex in vitro, and their cytoplasmic domains can specifically interact in a yeast two‐hybrid system. In vitro complex formation with catalytically active T beta R‐II is necessary and sufficient for T beta R‐I phosphorylation, which within this complex does not require the catalytic activity of T beta R‐I, thus mimicking T beta R‐I phosphorylation in intact cells. In addition, T beta R‐I phosphorylated in vitro remains associated with T beta R‐II. These results suggest that T beta R‐I and T beta R‐II have affinity for each other, however, the ligand is required for stable complex formation under physiological conditions. Once formed, this complex is sufficient for T beta R‐I phosphorylation by T beta R‐II.

Baseline resource information in an easily accessible form is a vital starting point for developing coastal zone management. On behalf of a wide-ranging group of organizations involved in the management of the U.K. maritime zone, the Joint Nature Conservation Committee (JNCC) is compiling a series of 16 regional volumes. The volumes contain multidisciplinary coastal zone resource information arranged in ten chapters under the general title ‘Coasts and Seas of the UK’. These volumes form part of a series of paper and electronic publications of coastal information being produced by JNCC's Coastal Directories Project and are designed to meet the needs of planners and all others involved in decision-making and management of the coast.

Background: The management of localized soft‐tissue sarcomas remains complex. This is a retrospective review of a single institution experience with manual afterloaded brachytherapy following intra‐operative implantation of the tumour bed during surgery.

Transforming growth factor beta (TGF-beta) signals through two transmembrane serine/threonine kinases, known as TbetaR-I and TbetaR-II. Several lines of evidence suggest that TbetaR-II acts as a primary receptor, binding TGF-beta and phosphorylating TbetaR-I whose kinase activity then propagates the signal to unknown substrates. We report an interaction between TbetaR-I and the farnesyl-protein transferase-alpha subunit (FT-alpha) both in a yeast two-hybrid system and in mammalian cells. These findings raise the possibility that TGF-beta might regulate cellular functions by altering the ability of FT-alpha to catalyze isoprenylation of targets such as G proteins, lamins, or cytoskeletal components. However, we provide evidence that TGF-beta action does not alter the overall protein isoprenyl transferase activity in Mv1Lu mink lung epithelial cells. In fact, the beta subunits of farnesyl transferase and geranylgeranyl transferase, which are necessary for the activity of FT-alpha, prevent the association of FT-alpha with TbetaR-I. Furthermore, farnesyl transferase activity is shown to be dispensable for TGF-beta signaling of growth inhibitory and transcriptional responses in these cells. These results suggest that the interaction between TbetaR-I and FT-alpha does not affect the known functions of these two proteins.

Bone morphogenic proteins (BMPs) are universal regulators of animal development. We report the identification and cloning of the BMP type II receptor (BMPR-II), a missing component of this receptor system in vertebrates. BMPR-II is a transmembrane serine/threonine kinase that binds BMP-2 and BMP-7 in association with multiple type I receptors, including BMPR-IA/Brk1, BMPR-IB, and ActR-I, which is also an activin type I receptor. Cloning of BMPR-II resulted from a strong interaction of its cytoplasmic domain with diverse transforming growth factor beta family type I receptor cytoplasmic domains in a yeast two-hybrid system. In mammalian cells, however, the interaction of BMPR-II is restricted to BMP type I receptors and is ligand dependent. BMPR-II binds BMP-2 and -7 on its own, but binding is enhanced by coexpression of type I BMP receptors. BMP-2 and BMP-7 can induce a transcriptional response when added to cells coexpressing ActR-I and BMPR-II but not to cells expressing either receptor alone. The kinase activity of both receptors is essential for signaling. Thus, despite their ability to bind to type I and II receptors receptors separately, BMPs appear to require the cooperation of these two receptors for optimal binding and for signal transduction. The combinatorial nature of these receptors and their capacity to crosstalk with the activin receptor system may underlie the multifunctional nature of their ligands.

Bone morphogenetic proteins (BMPs) are members of the TGF-beta family that regulate cell proliferation, apoptosis, and differentiation, and participate in the development of most tissues and organs in vertebrates. Smad proteins function downstream of TGF-beta receptor serine/threonine kinases and undergo serine phosphorylation in response to receptor activation. Smad1 is regulated in this fashion by BMP receptors, and Smad2 and Smad3 by TGF-beta and activin receptors. Here, we report that BMP receptors phosphorylate and activate Smad1 directly. Phosphorylation of Smad1 in vivo involves serines in the carboxy-terminal motif SSXS. These residues are phosphorylated directly by a BMP type I receptor in vitro. Mutation of these carboxy-terminal serines prevents several Smad1 activation events, namely, Smad1 association with the related protein DPC4, accumulation in the nucleus, and gain of transcriptional activity. Similar carboxy-terminal serines in Smad2 are required for its phosphorylation and association with DPC4 in response to TGF-beta, indicating the generality of this process of Smad activation. As a direct physiological substrate of BMP receptors, Smad1 provides a link between receptor serine/threonine kinases and the nucleus.

A convenient one-pot synthesis of 4-fluoroquinolinones that are active against KDR kinase is described. The mechanism of the reaction is believed to involve the formation of a quinone methide intermediate.