Search

The CNS immune response to rabies virus has been shown to be influenced by virulence of the virus strains. There is no comprehensive report of the peripheral immune response against different strains of rabies virus. In this report we used a comparative proteome analysis to find the early events in the spleen lymphocytes of mice infected by a street strain and an attenuated strain of the rabies virus. Differentially expressed proteins were identified which play important biological roles such as T and B lymphocyte activation (coronin 1), antiviral activity (peroxiredoxin 1), and cytoskeletal reorganization (cofilin 1). These results could be strong hints of early divergence on peripheral immune response under influence of viral strain and their pathogenicity.The CNS immune response to rabies virus has been shown to be influenced by virulence of the virus strains. There is no comprehensive report of the peripheral immune response against different strains of rabies virus. In this report we used a comparative proteome analysis to find the early events in the spleen lymphocytes of mice infected by a street strain and an attenuated strain of the rabies virus. Differentially expressed proteins were identified which play important biological roles such as T and B lymphocyte activation (coronin 1), antiviral activity (peroxiredoxin 1), and cytoskeletal reorganization (cofilin 1). These results could be strong hints of early divergence on peripheral immune response under influence of viral strain and their pathogenicity.

Prefractionation of complex protein mixtures is an efficient method for increasing the separation power of 2‐DE. RP‐HPLC has been successfully utilized as a prefractionation method prior to 2‐DE. Here we describe the optimization of an efficient RP‐HPLC method for prefractionation of baby hamster kidney cell solubilized proteins. A step gradient elution of acetonitrile was optimized and collected fractions were further examined by SDS‐PAGE and 2‐DE. By utilizing this method an effective increase in separation power of 2‐DE is accomplished. Moreover, we describe the application of this method to expressional proteome analysis of a virally infected cell model.