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Multitasking is a common feature of many modern work and home environments, and this study investigated the relationship between multitasking performance in two different paradigms: a more controlled task-switching paradigm (TSWP) and a more complex, semi-ecologically valid multitasking paradigm (SynWin). The study also explored whether parallel processors may have performance advantages in a complex dual-task environment. Results showed no significant correlation between individual multitasking efficiency in the TSWP and SynWin paradigms. Additionally, the results indicated that the combination of subtasks was the primary factor affecting performance in dual-task variants of the SynWin, rather than the use of a parallel processing mode. We conclude that there may be constraints with respect to the experimental conditions necessary to generalize findings from controlled multitasking paradigms to semi-ecologically valid tasks scenarios. Future research should prioritize efforts to understand how people multitask in more realistic settings and the underlying cognitive processes involved.

Introduction:The goal of early predictive safety assessment (PSA) is to keep compounds with detectable liabilities from progressing further in the pipeline. Such compounds jeopardize the core of pharmaceutical research and development and limit the timely delivery of innovative therapeutics to the patient. Computational methods are increasingly used to help understand observed data, generate new testable hypotheses of relevance to safety pharmacology, and supplement and replace costly and time-consuming experimental procedures.Areas covered:The authors survey methods operating on different scales of both physical extension and complexity. After discussing methods used to predict liabilities associated with structures of individual compounds, the article reviews the use of adverse event data and safety profiling panels. Finally, the authors examine the complexities of toxicology data from animal experiments and how these data can be mined.Expert opinion:A significant obstacle for data-driven safety assessment is the absence of integrated data sets due to a lack of sharing of data and of using standard ontologies for data relevant to safety assessment. Informed decisions to derive focused sets of compounds can help to avoid compound liabilities in screening campaigns, and improved hit assessment of such campaigns can benefit the early termination of undesirable compounds.

Betweensample variation in highthroughput flow cytometry data poses a significant challenge for analysis of largescale data sets, such as those derived from multicenter clinical trials. It is often hard to match biologically relevant cell populations across samples because of technical variation in sample acquisition and instrumentation differences. Thus, normalization of data is a critical step before analysis, particularly in largescale data sets from clinical trials, where groupspecific differences may be subtle and patienttopatient variation common. We have developed two normalization methods that remove technical betweensample variation by aligning prominent features landmarks in the raw data on a perchannel basis. These algorithms were tested on two independent flow cytometry data sets by comparing manually gated data, either individually for each sample or using static gating templates, before and after normalization. Our results show a marked improvement in the overlap between manual and static gating when the data are normalized, thereby facilitating the use of automated analyses on large flow cytometry data sets. Such automated analyses are essential for highthroughput flow cytometry. © 2009 International Society for Advancement of Cytometry

After sequencing the human genome, the challenge ahead is to systematically analyze the functions and disease relation of the proteins encoded. Here the authors describe the application of a flow cytometry–based high-throughput assay to screen for apoptosis-activating proteins in transiently transfected cells. The assay is based on the detection of activated caspase-3 with a specific antibody, in cells overexpressing proteins tagged C- or N-terminally with yellow fluorescent protein. Fluorescence intensities are measured using a flow cytometer integrated with a high-throughput autosampler. The applicability of this screen has been tested in a pilot screen with 200 proteins. The candidate proteins were all verified in an independent microscopy-based nuclear fragmentation assay, finally resulting in the identification of 6 apoptosis inducers.