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This study was conducted to evaluate the insecticidal activity of the endophytic fungus Isaria fumosorosea Wize isolated from Ricinus communis L. against Locusta migratoria L. and Acanthoscelides obtectus Say. To evaluate the insecticidal activity, a mixture of fungal extract and spore suspensions (1x107, 1x106 and 1x105 spores/ml of dissolved extract) were applied against the insects. It was found that I. fumosorosea had a good insecticidal activity, positively proportional to concentration and time after exposure (24, 48, 72 hours). A maximum mortality rate of 80% for A. obtectus and 70% for L. migratoria at 1x107 spores/ml of dissolved extract was recorded. Preliminary phytochemical analyses of the fungal extract revealed the presence of alkaloids, flavonoids and phenols. Additionally, a fungal development on the insects bodies has also been noticed; that is undoubtedly explained the recorded insecticidal activity.

This study was conducted to evaluate anti-acetylcholinesterase and insecticidal and antifungal activities of the endophytic fungus Trichoderma sp, isolated from Ricinus communis L. leaves, against Locusta migratoria L. and Botrytis cinerea Pers.: Fr.. To evaluate the insecticidal and antifungal activities, different concentrations of the fungal extract were applied against L. migratoria (0.2, 0.3, 0.4 g/l) and against B. cinerea (1, 2, 3 g/l). It was found that the mortality of the targeted insects was positively proportional to fungal extract concentration and time after exposure (24, 48, 72 hours). The concentration 0.4 g/l appeared to be the most effective after 72 hours with mortality rate of 56.52%. Regarding antifungal activity, the concentration 3 g/l was the most effective against B. cinerea after 7 days, with an inhibition rate of 92.06% (excellent antifungal activity). Moreover, it was found that at 4 ug/ml the fungal extract had a maximum inhibitory capacity of Ache of 80% for acetylcholenesterase. Preliminary phytochemical analyses revealed the presence of alkaloids, flavonoids, phenols and saponins. In addition the colony of this endophytic fungus produced chitinases and proteases, which explained its important antifungal and insecticidal activities.