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The objectives of this work were to optimize hydro-distillation extraction of Salvia officinalisL. essential oil (SoEO) and to evaluate the in vitro scavenging capacity of the free radicals DPPH·, NO·, ABTS+, and the ability to reduce Fe3+. The optimization of SoEO extraction by hydro-distillation was carried out using the response-surface methodology by Box–Behnken. The cytotoxicity and anti-proliferative capacities were evaluated by measuring cell viability and then modeled. Two human tumor cell lines: MCF-7 and HeLa were involved. The obtained optimal extraction yield was Y = 1.85 g/100 g d.b. The main identified fractions were camphene (23.7%), α-thujone (19.62%), 1,8-cineole (10.6%), viridiflorol (5.9%), borneol (5.72%), β-thujone (5.4%) and caryophyllene (3.83%). Also, SoEO was mostly able to scavenge DPPH·free radical, ABTS+radical and hydrogen peroxide in an amount dependent manner (IC50= 0.97, 0.279 and 0.05 mg/mL, respectively). All treated cell lines showed a significant reduction in cell viability in response to the increasing of oil concentration. The cytotoxicity activity against both tumor cell lines MCF-7 and HeLa was considerably important with IC50= 3.125 and 8.920 μg/mL, respectively. The present work provides a preliminary platform for further investigation of the possible mechanism of S. officinalisessential oils and their individual compounds in cytotoxic and antitumor activities.

Objectives: The aim of this study was to explore the oxidative stress profile in hashitoxicosis (HTX) and to compare it with that of healthy subjects.Patients and methods: Spectrophotometric methods were used to evaluate the oxidative stress markers. The selenium level was investigated by atomic absorption.Results: High levels of thiobarbituric acid reactive species (TBARS) and conjugated dienes were found in HTX patients (p =0.034 and p =0.043, respectively) compared with healthy controls. For antioxidant enzymes, superoxide dismutase (SOD) and catalase activities increased, whereas that of glutathione peroxidase (GPx) decreased (p =0.000, p =0.014, p =0.000, respectively) compared with controls. A reduction in the level of selenium (p =0.029) and thiol groups (p =0.008) were shown in patients; however, levels of carbonyl group and malondialdehyde (MDA) protein adducts decreased (p =0.000) compared with controls. Positive correlation was shown between levels of free thyroxine (FT4) and TBARS (r =0.711, p =0.048) and between FT4 level and SOD activity (r =0.713, p =0.047). Conversely, GPx activity presented a negative correlation with FT4 and free triiodothyronine (FT3) levels (r =–0.934, p =0.001; r =–0.993, p =0.000, respectively). In addition, GPx activity showed positive correlation with selenium level (r =0.981, p =0.019) and the FT3 level correlated negatively with the level of thiol groups (r =–0.892, p =0.017).Conclusions: This study shows the presence of an oxidative stress and selenium deficiency in HTX patients and suggests that the hyperthyroid state is strongly implicated in the establishment of this disturbed oxidative profile.