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The high host genetic background of tissue biopsies hinders the application of shotgun metagenomic sequencingin characterizing the tissue microbiota. We proposed an optimized method that removed host DNA from colon biopsies and examined the effect on metagenomic analysis. Human or mouse colon biopsies were divided into two groups, with one group undergoing host DNA depletionand the other serving as the control. Host DNA was removed through differential lysis of mammalian and bacterial cells before sequencing. The impact of host DNA depletion on microbiota was compared based on phylogenetic diversity analyses and regression analyses. Removing host DNA enhanced bacterial sequencing depth and improved species discovery, increasing bacterial reads by 2.46 ± 0.20 folds while reducing host reads by 6.80% ± 1.06%. Moreover, 2.40 times more of bacterial species were detected after host DNA depletion. This was confirmed from mouse colon tissues, increasing bacterial reads by 5.46 ± 0.42 folds while decreasing host reads by 10.2% ± 0.83%. Similarly, significantly more bacterial species were detected in the mouse colon tissue upon host DNA depletion (P< 0.001). Furthermore, an increased microbial richness was evident in the host DNA-depleted samples compared with non-depleted controls in human colon biopsies and mouse colon tissues (P< 0.001). Our optimized method of host DNA depletion improves the sensitivity of shotgun metagenomic sequencing in bacteria detection in the biopsy, which may yield a more accurate taxonomic profile of the tissue microbiota and identify bacteria that are important for disease initiation or progression.

The high host genetic background of tissue biopsies hinders the application of shotgun metagenomic sequencingin characterizing the tissue microbiota. We proposed an optimized method that removed host DNA from colon biopsies and examined the effect on metagenomic analysis. Human or mouse colon biopsies were divided into two groups, with one group undergoing host DNA depletionand the other serving as the control. Host DNAs were removed through differential lysis of mammalian and bacterial cells before sequencing. The impact of host DNA depletion on microbiota was compared based on phylogenetic diversity analyses and regression analyses. Removing host DNA enhanced bacterial sequencing depth and improved species discovery, increasing bacterial reads by 2.46 ± 0.20 fold while reducing host reads by 6.80% ± 1.06%. Moreover, 3.40 times more of bacterial species were detected after host DNA depletion. This was confirmed from mouse colon tissues, increasing bacterial reads by 5.46 ± 0.42 fold while decreasing host reads by 10.2% ± 0.83%. Similarly, significantly more species were detected in the mouse colon tissue upon host DNA depletion (P < 0.001). Furthermore, an increased microbial richness was evident in the host DNA-depleted samples compared with non-depleted controls in human colon biopsies and mouse colon tissues (P < 0.001). Our optimized method of host DNA depletion improved the sensitivity of shotgun metagenomic sequencing in bacterial detection in the biopsy, which may yield a more accurate taxonomic profile of the tissue microbiota and identify bacteria that are important for disease initiation or progression.

According to the problem of bionic shark gill jet can reduce friction on shark surface, a model of bionic jet surface was established based on shark surface was analyzed by measurements, and its numerical simulation was processed by using RNG k-ε turbulence model. The results show that: the gill jet can reduce frictional resistance on shark surface, and the best drag reduction can be got when the speed of main field is 5m/s, furthermore the maximum drag reduction rate can be up to 17.15%. The pressure of jet hole upstream is reduced which due to the barrier to the facing fluid by the jet, so that the pressure resistance of jet surface is reduced as well. Besides, jet fluid is blocked in the boundary layer by mainstream fluid, which caused the fluid velocity of jet hole downstream is reduced, the thickness of boundary layer is increased, and the normal velocity gradient of wall is reduced, so as to achieve the effect of drag reduction.

According to the problem of drag reduction on bionic jet surface, a rectangular jet surface model which is similar to shark branchial shape was built, and numerical simulation was processed by using SST k-ω turbulence model, moreover, influence of jet hole size on the drag reduction performance of jet surface was studied. The results show that: the effect of flow length of rectangular jet hole on the drag reduction is remarkable, with the increase of flow length, fluid friction resistance of the jet surface decreases, the maximum drag reduction rate was 14.38%, and the results of numerical simulation was verified by carrying out experiments. The jet fluid decreases the sweep on the wall of mainstream high speed fluid, which increases the thickness of jet surface boundary layer, thereby reducing the surface friction of the jet hole downstream.

The cross-section of yarn in plain woven fabric has always been viewed as an invariable shape by academe. However, with visualization technology, problems in the classical model are exposed based on traditional convex model, and when solving the problems with calculus, the rule of yarn cross-section deformation is found. The results show the diversity of yarn cross-section shape in both one-layer and multi-layer woven fabric, instead of unitarity, especially for warps weaving multi-layer wefts, whose convex cross-section alternates with concave one periodically in length. The results take exception to “invariant cross-section assumption” and have theoretical value and guidance for the real shape of yarns and the simulation of fabric appearance.

A measurement system utilizing laser frequency splitting is presented for the precise measurement of retardations of arbitrary wave plates. The wave plate to be measured is inserted into a laser resonance cavity, and its retardation can be determined by measuring the difference of split frequencies and longitudinal mode spacing of the laser. In the system, mode spacing measurement is performed under multimode oscillation according to the relation between polarization directions of modes. To solve the problem of strong mode competition in measuring half-wave plates, a transverse magnetic field is applied to weaken the competition so as to make the system applicable even when the frequency difference is less than the threshold for strong mode competition. The error of the system is less than 5 arcmin, and standard deviations of about 1 arcmin are obtained from repeated experiments samples of quarter- and half-wave plates. No element rotation is required during measurement, and no mechanical quantity, such as angle, needs to be measured.