1. Linking transcriptional dynamics of CH4-cycling grassland soil microbiomes to seasonal gas fluxes
- Author
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Täumer, Jana, Marhan, Sven, Groß, Verena, Jensen, Corinna, Kuss, Andreas W, Kolb, Steffen, and Urich, Tim
- Abstract
Soil CH4fluxes are driven by CH4-producing and -consuming microorganisms that determine whether soils are sources or sinks of this potent greenhouse gas. To date, a comprehensive understanding of underlying microbiome dynamics has rarely been obtained in situ. Using quantitative metatranscriptomics, we aimed to link CH4-cycling microbiomes to net surface CH4fluxes throughout a year in two grassland soils. CH4fluxes were highly dynamic: both soils were net CH4sources in autumn and winter and sinks in spring and summer, respectively. Correspondingly, methanogen mRNA abundances per gram soil correlated well with CH4fluxes. Methanotroph to methanogen mRNA ratios were higher in spring and summer, when the soils acted as net CH4sinks. CH4uptake was associated with an increased proportion of USCa and ? pmoAand pmoA2 transcripts. We assume that methanogen transcript abundance may be useful to approximate changes in net surface CH4emissions from grassland soils. High methanotroph to methanogen ratios would indicate CH4sink properties. Our study links for the first time the seasonal transcriptional dynamics of CH4-cycling soil microbiomes to gas fluxes in situ. It suggests mRNA transcript abundances as promising indicators of dynamic ecosystem-level processes.
- Published
- 2022
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