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Recent publications indicate that the Amazon may be acting more as a carbon source than a sink in some regions. Moreover, the Amazon is a source of moisture for other regions in the continent, and deforestation over the years may be reducing this function. In this work, we analyze the impacts of elevated CO2(eCO2) and land use change (LUC) on gross primary productivity (GPP) and evaporation in the southern Amazon (7°S 14°S, 66°W 51°W), which suffered strong anthropogenic influence in the period of 1981‒2010. We ran four dynamic global vegetation models (DGVMs), isolating historical CO2, constant CO2, LUC, and potential natural vegetation scenarios with three climate variable data sets: precipitation, temperature, and shortwave radiation. We compared the outputs to five “observational” data sets obtained through eddy covariance, remote sensing, meteorological measurements, and machine learning. The results indicate that eCO2may have offset deforestation, with GPP increasing by ∼13.5% and 9.3% (dry and rainy seasons, respectively). After isolating the LUC effect, a reduction in evaporation of ∼4% and ∼1.2% (dry and rainy seasons, respectively) was observed. The analysis of forcings in subregions under strong anthropogenic impact revealed a reduction in precipitation of ∼15 and 30 mm, and a temperature rise of 1°C and 0.6°C (dry and rainy seasons, respectively). Differences in the implementation of plant physiology and leaf area index in the DGVMs introduced some uncertainties in the interpretation of the results. Nevertheless, we consider that it was an important exercise given the relevance. The elevated atmospheric CO2had a strong positive influence on gross primary productivity (GPP) and also offset deforestation, when native forest was converted to grassesLand use change also had a negative effect on evaporation and most intense effects of climate change (as temperature increasing) were on deforested areasUncertainties were found in the outputs of the models and in the climatic forcings. Divergences were also observed among the models The elevated atmospheric CO2had a strong positive influence on gross primary productivity (GPP) and also offset deforestation, when native forest was converted to grasses Land use change also had a negative effect on evaporation and most intense effects of climate change (as temperature increasing) were on deforested areas Uncertainties were found in the outputs of the models and in the climatic forcings. Divergences were also observed among the models