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One of the long-standing challenges to performing electrogenerated chemiluminescence (ECL) research is the need for dedicated instrumentation or highly customized cells to achieve reproducibility. This manuscript describes an approach to designing ECL systems through the hyphenation of existing laboratory instruments, which provide innate time correlation of electrochemical and emission data. This design methodology lowers the entry barrier required to obtaining reproducible ECL measurements and provides flexibility in the scope of applications. Uniquely, the simplicity of this system’s experimental interface, a spectrochemical quartz cuvette, readily enables collaboration with finite element modeling that simulates ECL occurring in the cuvette-based cell. This combination of empirical and simulation data allowed for the investigation of the intertwined kinetics behind the coreactant ECL mechanism of tris(2,2′-bipyridine)ruthenium(II) (Ru(bpy)32+) and tripropylamine (TPA). The complexity of the system measurable via the hyphenation methodology was further scaled though the addition of tris[2-(4,6-difluorophenyl)pyridinato-C2, N] iridium(III) (Ir(dFppy)3) and the observation of real time multiplexing.

We report a new fabrication method to produce palladium hydride pH microelectrode using a chemical approach to synthesize the palladium hydride. In contrast to electrochemically generated palladium hydride microelectrodes, chemically generated palladium hydride microelectrodes are longer lasting and importantly have a good analytical performance under aerobic conditions. Chemically generated palladium hydride microelectrodes perform best in acid to neutral electrolytes devoid of Cl−. They can readily be produced on 10 μm diameter disk platinum microelectrodes, which makes them attractive candidates for future localized electrochemical studies.

A new type of an electrochemical flow cell designed for exchangeable ultramicroelectrodes (UMEs) is presented. The cell could be used with up to 8 different UMEs allowing one to use this cell for UME array measurements. The cell was characterized using both empirical data and finite element modeling. Au and Pt UMEs were used to detect catechol simultaneously, demonstrating the ability of this cell to be used as a sensory platform. The electroanalytical response of flow system was validated by direct measurements of H2O2 concentrations. Finally, stochastic nanoparticle measurements were successfully performed using the flow system, highlighting the future possibilities of this system for environmental monitoring.

The field of enzymatic biosensors applied to brain electrochemistry has rapidly expanded over the last few years, thanks in part to their excellent selectivity to specific target species. Much current research is therefore focused on enhancing the electrochemical signal, which often involves the detection of stoichiometric amounts of H2O2 formed as part of the enzyme mechanism. This opens the possibility of enhancing a biosensor's performance by facilitating the H2O2 oxidation signal through surface modification. Here, we investigate the impact of the roughness of the platinum surface on the biosensor response, where rougher platinum surfaces show greater activity for H2O2 oxidation, and therefore enhanced biosensor sensitivity. Through careful manipulation of the electrode surface roughness, we are able to show a significant improvement to the LOD when using a rougher electrode surface. Additionally, we have shown that this enhanced surface roughness has no detrimental effects toward the electrode response time. This suggests that surface roughness could be a simple and easy to implement means of enhancing the sensitivity of electrode-based enzymatic biosensors, and is an important factor to consider when studying other aspects of biosensor fabrication.

A new type of an electrochemical flow cell designed for exchangeable ultramicroelectrodes (UMEs) is presented. The cell could be used with up to 8 different UMEs allowing one to use this cell for UME array measurements. The cell was characterized using both empirical data and finite element modeling. Au and Pt UMEs were used to detect catechol simultaneously, demonstrating the ability of this cell to be used as a sensory platform. The electroanalytical response of flow system was validated by direct measurements of H2O2concentrations. Finally, stochastic nanoparticle measurements were successfully performed using the flow system, highlighting the future possibilities of this system for environmental monitoring.

The field of enzymatic biosensors applied to brain electrochemistry has rapidly expanded over the last few years, thanks in part to their excellent selectivity to specific target species. Much current research is therefore focused on enhancing the electrochemical signal, which often involves the detection of stoichiometric amounts of H2O2formed as part of the enzyme mechanism. This opens the possibility of enhancing a biosensor's performance by facilitating the H2O2oxidation signal through surface modification. Here, we investigate the impact of the roughness of the platinum surface on the biosensor response, where rougher platinum surfaces show greater activity for H2O2oxidation, and therefore enhanced biosensor sensitivity. Through careful manipulation of the electrode surface roughness, we are able to show a significant improvement to the LOD when using a rougher electrode surface. Additionally, we have shown that this enhanced surface roughness has no detrimental effects toward the electrode response time. This suggests that surface roughness could be a simple and easy to implement means of enhancing the sensitivity of electrode-based enzymatic biosensors, and is an important factor to consider when studying other aspects of biosensor fabrication.