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Damages of sensory hair cells (HCs) are mainly responsible for sensorineural hearing loss, while the pathological mechanism remains not fully understood due to the many potential deafness genes unidentified. ftr82, a member of the largely TRIMs family in fish, has been found specifically expressed in the otic vesicle while its function is still unclear. Here, we investigate the roles of ftr82in HC development and hearing function utilizing the zebrafish model. The results of in situ hybridization illustrate that ftr82is always restricted to localize in otic vesicles at different stages. The defects of HCs are observed both in ftr82morphants and mutants, including significantly decreased crista HCs, shortened cilia as well as remarkably reduced functional HCs in neuromasts, which could be successfully rescued by co-injection of exogenous ftr82 mRNA. The behavior assay of startle response indicates that larvae lacking of ftr82exhibits lower sensitivity to external sound stimuli. Further research reveals that the loss of HCs is mainly caused by cell apoptosis mediated by caspase-3 activation. Our study demonstrates that ftr82is a crucial hearing-related gene that regulates the HC morphogenesis and auditory function performing, which provides new insight into the rapid identification of the deafness gene.

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The plane exposure of support vitally affects the catalytic performance of the catalyst. In this work, CeO2nanorods ((110) plane exposure), nano-octahedrons ((111) plane exposure) and nano-cubes ((100) plane exposure) were prepared as the supports of Pt/CeO2samples to investigate the effect of CeO2plane exposure on total toluene oxidation. Characterizations reveal that the (110) plane of CeO2is more helpful to the dispersion of Pt species, followed by (111) face. The improved dispersion of Pt species can enhance the metal-supports interaction, which promotes the electron transfer of CeO2carrier to Pt nanoparticles and the adsorption-activation of O2, thereby facilitating the total oxidation of toluene via the Langmuir–Hinshelwood (L-H) mechanism. Therefore, Pt/CeO2-r (nanorods) sample expresses excellent catalytic performance of toluene oxidation. Finally, the procedure of toluene total oxidation was studied by in-situdiffuse reflectance infrared Fourier transform (DRIFT). We expect that this work can contribute to the development of an effective sample for the decomposition of volatile organic compounds (VOCs).