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In a prior study, we identified a novel sepsis specific long noncoding RNAs (lncRNA) RP11-284N8.3, which may primarily participate in T cell activation and immune response during sepsis. However, the clinical significance of lncRNA RP11-284N8.3 in sepsis remains entirely unknown. This single-center prospective cohort study enrolled 147 adults with sepsis and 74 healthy controls (HCs) with matched age and sex between January 2021 and November 2022 at our hospital. Blood samples and clinical data were collected from HCs at enrollment and from adults with sepsis within 24 hours after admission. lncRNA RP11-284N8.3 expression was detected by RT-qPCR. The relative expression of lncRNA RP11-284N8.3 was significantly decreased in adults with sepsis compared to HCs (P< .0001), in adults with septic shock compared to adults without shock (P= .0012), and in 28-day deaths compared to 28-day survivors (P= .0006). receiver operating characteristic curves of lncRNA RP11-284N8.3 in predicting sepsis severity and 28-day mortality showed an area under the curve of 0.6570 (95% confidence interval [CI]: 0.5701–0.7440) and an area under the curve of 0.6765 (95% CI: 0.5809–0.7721), respectively. Multivariate logistic regression analysis revealed that lncRNA RP11-284N8.3 was an independent risk factor for 28-day mortality in adults with sepsis (odds ratio: 0.1057, 95% CI: 0.0115–0.7746, P= .0328). Low expression of lncRNA RP11-284N8.3 is correlated with increased risk, severity and 28-day mortality in adults with sepsis, and it may function as a potential biomarker to facilitate the diagnosis and management of sepsis.

Water-soluble coordination polymer nanoparticles (Ir–Gd CPPs) were conveniently synthesized in high yields in the presence of polyvinylpyrrolidone (PVP), adopting magnetic Gd(III) ions, and phosphorescent iridium complexes with carboxyl groups as building blocks. The Ir–Gd CPPs showed good stability in simulated biological media and low cytotoxicity toward a model line of HeLa cancer cells. The Ir–Gd CPPs exhibited absorption in the visible region, red phosphorescence centered at 560 nm, and higher longitudinal relaxivity (r1) of ∼29.5 mM–1s–1in a 3 T MRI system. Furthermore, the effective uptake of Ir–Gd CPPs by HeLa cells was confirmed by confocal laser scanning microscopy and flow cytometry, suggesting they may be useful as an optical probe for living cells. The generation of 1O2upon irradiation with a visible light prompted an investigation into the possibility of using Ir–Gd CPPs in photodynamic therapy. After incubation with 200 μg mL–1of Ir–Gd CPPs for 6 h, the viability of HeLa cells was only ∼16.6% after irradiation with a visible light (λ > 400 nm, 300 mW cm–2).

Antibody repertoire sequencing enables researchers to acquire millions of B cell receptors and investigate these molecules at the single-nucleotide level. This power and resolution in studying humoral responses have led to its wide applications. However, most of these studies were conducted with a limited number of samples. Given the extraordinary diversity, assessment of these key features with a large sample set is demanded. Thus, we collect and systematically analyze 2,152 high-quality heavy-chain antibody repertoires. Our study reveals that 52 core variable genes universally contribute to more than 99% of each individual’s repertoire; a distal interspersed preferences characterize V gene recombination; the number of public clones between two repertoires follows a linear model, and the positive selection dominates at RGYW motif in somatic hypermutations. Thus, this population-level analysis resolves some critical features of the antibody repertoire and may have significant value to the large cadre of scientists.