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The goal of the work was to determine and characterize the inclusions of nickel sulphide in tempered glass. During the tempering process where the glass is heated the nickel sulphide stones are completed transformed to the high temperature modification which is unstable when the temperature decreased. The glass samples, which include the NiS stones, were investigated by microscopic method (SEM) and characterized by EDAX analysis. The glass samples were analysis by elastooptic studies but it did not prove occurrence of stresses. Obtained results will help to solve the problem with forming nickel sulphide inclusions.