Search

To construct a recombinant plasmid Pet23a-M, the gene encoding severe acute respiratory syndrome (SARS) coronavirus membrane protein was amplified by RT-PCR and cloned into the expression plasmid Pet23a. Results of restriction endonuclease analysis, PCR detection and DNA sequencing analysis revealed that the cloned DNA sequence was the same as that reported. The recombinants were transformed intoEscherichia coli(E. Coli) BL21 (DE3) and induced by Isopropyl-β-D-thiogalactopyranoside (IPTG). The expression of 27 kD (1 kD=0.992 1 ku) protein was detected by SDS-PAGE and pured by metal chelated chromatography. Results of Western-blot showed that this expressed protein could react with antibodies in sera of SARS patients during convalescence. This provided the basis for the further study on SARS virus vaccine and diagnostic agents.

A signal waveform recovery method based on the co-prime array is investigated to extract the waveform of the desired signal from spatial interferences in narrowband scenarios. The direction of arrivals (DOAs) of the desired signal and interference signals are estimated with the compressive sensing approach based on angle grids, and the signal power together with the noise power are estimated. Thereafter, a modified steepest descent (SD) method is derived to recover the waveform of the desired signal and interferences utilizing the estimated power and directions. The recovered waveform of the desired signal Is the output of the proposed method. The situation in which the signals are not on the predefined angle grids is also considered. The DOAs estimated via the predefined angle grids are corrected based on the maximum likelihood (ML) angle estimation. Compared to the existing beamforming methods on co-prime array, the proposed method can obtain the waveform of the desired signal. Simulation results demonstrate that the proposed method can achieve good performance in signal waveform recovery and output signal to noise ratio. [ABSTRACT FROM AUTHOR]