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•Human occupation in moments immediately before the extinction of the megafauna.•Wide faunal diversity in Patagonia towards the end of the Pleistocene.•Evidence of human use in current and extinct camelids.•Complementary use of other animals.•Megafauna was another resource to be exploited within a broad-spectrum economy.

•Feces and coprolites of camelids were studied by plant remains and pollen analyses.•Plant remains and pollen data suggests the use of the grass-shrub steppe.•The results contribute information for the relationship between hunter-gatherers and camelids.•The results provide new detailed information for paleoenvironmental reconstructions.

•We re-analyze the isotope values presented by Gil et al. (2020a).•The spatial grouping of human samples influence results and interpretations.•Diet estimations change with number and isotopic similarity of resources.