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La infección por COVID-19 altera principalmente la función pulmonar, aunque también se han reconocido manifestaciones extrapulmonares. Hasta el momento se sabe que una de las complicaciones cardiovasculares más comunes asociadas al COVID-19 es la pericarditis, que puede presentarse en su severidad extrema con taponamiento cardiaco, el cual puede tener un desenlace fatal si no es reconocido y tratado a tiempo. En el actual reporte de caso describimos el caso anecdótico de una paciente de 39 años, sin antecedentes patológicos de importancia, quien previamente ha recibido 2 dosis de ARNm-1273 para SARS-CoV-2 con la última dosis aplicada al menos 3 meses después de un cuadro clínico compatible con taponamiento cardiaco secundario a pericarditis por SARS-CoV-2; su evolución fue satisfactoria tras drenaje por videotoracoscopia del líquido pericárdico, así como uso de colchicina.

Single nucleotide polymorphisms (SNPs) are the most common sequence difference found in plant genomes, yet they have not been widely exploited for producing molecular markers in common bean (Phaseolus vulgarisL.). The objective of this study was to develop a SNP assay based on a type of heteroduplex mismatch cleavage called EcoTILLING for molecular marker development in this important legume, and apply the assay (i) to the conversion of a sequence‐characterized amplified region (SCAR) marker useful for selecting virus resistance (SR2) and (ii) to the screening of SNP polymorphisms in newly developed expressed sequence tag (EST)–based markers. The SNP assay involved heteroduplex mismatch cleavage by a single‐strand specific nuclease ‘CEL I’ which was used to uncover two SNPs in the SR2 fragment and 22 SNPs in 37 candidate ESTs, some of which were used in segregation analysis. While developing the SNP techniques we tested several platforms, including LI‐COR, nondenaturing polyacrylamide, and agarose gel detection. The agarose gel system was used for SNP genetic mapping in two common bean mapping populations, showing that heteroduplex cleavage is a useful technique for increasing molecular marker number for the crop. Examples are given of mapped SNP markers for the phytic acid pathway gene for myo‐inositol‐1‐phosphate synthase and a drought tolerance–related gene, S‐adenosylmethionine decarboxylase.

A total of 120, twelve wk old female Japanese quails were divided into 4 groups (6 replicates of 5 birds each). The control group (CON) fed a corn-soybean diet; in the other 3 groups, Tenebrio molitorlarvae meal (TML) replaced 5, 10, and 20% of the soybean protein (T5, T10, and T20). The laying performance and egg quality were studied for 54 d. The data were processed by a one-way ANOVA; the orthogonal contrast analysis was performed to test the linear, quadratic and cubic effects among the means. The laying rate and egg mass linearly decreased (P< 0.01) as the TML inclusion level in the diet increased. The egg weight and feed conversion ratio linearly increased from the control to T20 diet (P< 0.01) while the digestibility of dry matter, organic matter, and crude protein linearly decreased (P< 0.05). The albumen and yolk weight showed a linear increase (P< 0.01) due to dietary TML inclusion, while the eggshell weight showed the opposite (P< 0.05). The estimated activity of Δ9-desaturase (C16:0), Δ5+Δ6-desaturase on both polyunsaturated fatty acid n-6 and n-3 linearly increased (P< 0.05) as affected by dietary TML. The boiled yolk lightness (L*) showed higher values in T5 and T10 groups (quadratic contrast, P< 0.01). The yolk redness index (a*) showed lower values in T5 and T20 than control and T10 groups (cubic contrast, P< 0.01). The albumen L*, a*, and b*indexes showed a significant effect of the quadratic contrast (P< 0.05). In addition, the albumen b*index showed a significant effect of the cubic contrast (P< 0.01). The total lipids showed the highest values (cubic contrast, P< 0.05) in the T10 and T20 groups. The total monounsaturated fatty acids linearly increased (P< 0.05) according to the increase of dietary TML. The best inclusion level of defatted TML meal for laying quails seems to be 1.4% of diet, corresponding to the T5 diet.