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•Intrathecal interferon β (IFNβ) attenuates mechanical allodynia without behavioral deficits.•Interferon-stimulated gene 15 (ISG15) is induced by IFNβ in the spinal cord and mediates analgesic effects.•IFNβ mediated analgesia and induction of ISG15 is potentiated in UBP43−/−mice.•IFNβ treatment inhibits MAPK signaling by ISG15 activation.

Antioxidant activity of the flavonoids from pomelo (Citrus grandis (L.) Osbeck) peel obtained by conventional soxhlet extraction (CSE), microwave-assisted extraction (MAE) and supercritical carbon dioxide (SC-CO2) extraction were investigated. For measuring antioxidant activities, different methods have been used corresponding to different levels of antioxidant action. The results indicated that the extraction yields of flavonoids by MAE and SC-CO2 extraction were higher than that by CSE method, whereas flavoniods by SC-CO2 extraction exhibited the strongest antioxidant activities. Our results indicated that SC-CO2 extraction method could effectively reserve the antioxidant ability of pomelo peel flavonoids.