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We report the generation of an organism-wide catalog of 976,813 cis-acting regulatory elements for the bovine detected by the assay for transposase accessible chromatin using sequencing (ATAC-seq). We regroup these regulatory elements in 16 components by nonnegative matrix factorization. Correlation between the genome-wide density of peaks and transcription start sites, correlation between peak accessibility and expression of neighboring genes, and enrichment in transcription factor binding motifs support their regulatory potential. Using a previously established catalog of 12,736,643 variants, we show that the proportion of single-nucleotide polymorphisms mapping to ATAC-seq peaks is higher than expected and that this is owing to an approximately 1.3-fold higher mutation rate within peaks. Their site frequency spectrum indicates that variants in ATAC-seq peaks are subject to purifying selection. We generate eQTL data sets for liver and blood and show that variants that drive eQTL fall into liver- and blood-specific ATAC-seq peaks more often than expected by chance. We combine ATAC-seq and eQTL data to estimate that the proportion of regulatory variants mapping to ATAC-seq peaks is approximately one in three and that the proportion of variants mapping to ATAC-seq peaks that are regulatory is approximately one in 25. We discuss the implication of these findings on the utility of ATAC-seq information to improve the accuracy of genomic selection.

To test the hypothesis that gender affects horse scores in eventing competition, data on the scores and points awarded to 681 horses was collected from the British Eventing website. Equal numbers of mares, geldings and stallions were used, all foaled during or after 1994 and aged 4–10 years. The study included five levels of competition (BE90, BE100, Novice, Intermediate and Advanced) and investigated differences in mean phased scores, total scores and rank in competition. Additionally, the mean and median ‘BE points per competition’ of each gender were compared. Significant differences in performance between genders were found at all levels except Advanced. Differences were highlighted in average phased and total scores, rank and median points per competition. There was an overall pattern of stallions and geldings outperforming mares, though this was not found to be true at all levels. The only area in which mares were found to perform significantly better than geldings or stallions was showjumping time penalties at BE90.