19 results on '"Shen, Mingqiang"'
Search Results
2. Transcription factor Nkx2-3 maintains the self-renewal of hematopoietic stem cells by regulating mitophagy
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Hu, Mengjia, Chen, Naicheng, Chen, Mo, Chen, Fang, Lu, Yukai, Xu, Yang, Yang, Lijing, Zeng, Hao, Shen, Mingqiang, Chen, Xuehong, Chen, Shilei, Wang, Fengchao, Wang, Song, and Wang, Junping
- Abstract
Hematopoietic stem cells (HSCs) reside at the top of the hematopoietic hierarchy, exhibiting a unique capacity to self-renew and differentiate into all blood cells throughout the lifetime. However, how to prevent HSC exhaustion during long-term hematopoietic output is not fully understood. Here, we show that the homeobox transcription factor Nkx2-3 is required for HSC self-renewal by preserving metabolic fitness. We found that Nkx2-3 is preferentially expressed in HSCs with excessive regenerative potential. Mice with conditional deletion of Nkx2-3 displayed a reduced HSC pool and long-term repopulating capacity as well as increased sensitivity to irradiation and 5-flurouracil treatment due to impaired HSC quiescence. In contrast, overexpression of Nkx2-3 improved HSC function both in vitro and in vivo. Furthermore, mechanistic studies revealed that Nkx2-3 can directly control the transcription of the critical mitophagy regulator ULK1, which is essential for sustaining metabolic homeostasis in HSCs by clearing activated mitochondria. More importantly, a similar regulatory role of NKX2-3 was observed in human cord blood-derived HSCs. In conclusion, our data demonstrate an important role of the Nkx2-3/ULK1/mitophagy axis in regulating the self-renewal of HSCs, therefore providing a promising strategy to improve the function of HSCs in the clinic.
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- 2023
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3. Akt-mediated mitochondrial metabolism regulates proplatelet formation and platelet shedding post vasopressin exposure
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Chen, Shilei, Sun, Kangfu, Xu, Baichuan, Han, Songlin, Wang, Song, Xu, Yang, Chen, Fang, Chen, Mo, Shen, Mingqiang, Lu, Yukai, Du, Changhong, Hu, Mengjia, Wang, Fengchao, and Wang, Junping
- Abstract
Platelet shedding from mature megakaryocytes (MKs) in thrombopoiesis is the critical step for elevating circulating platelets fast and efficiently, however, the underlying mechanism is still not well-illustrated, and the therapeutic targets and candidates are even less.
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- 2023
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4. Tespa1 facilitates hematopoietic and leukemic stem cell maintenance by restricting c-Myc degradation
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Lu, Yukai, Yang, Lijing, Shen, Mingqiang, Zhang, Zihao, Wang, Song, Chen, Fang, Chen, Naicheng, Xu, Yang, Zeng, Hao, Chen, Mo, Chen, Shilei, Wang, Fengchao, Hu, Mengjia, and Wang, Junping
- Abstract
Hematopoietic stem cells (HSCs) and leukemia stem cells (LSCs) have robust self-renewal potential, which is responsible for sustaining normal and malignant hematopoiesis, respectively. Although considerable efforts have been made to explore the regulation of HSC and LSC maintenance, the underlying molecular mechanism remains obscure. Here, we observe that the expression of thymocyte-expressed, positive selection-associated 1 (Tespa1) is markedly increased in HSCs after stresses exposure. Of note, deletion of Tespa1 results in short-term expansion but long-term exhaustion of HSCs in mice under stress conditions due to impaired quiescence. Mechanistically, Tespa1 can interact with CSN subunit 6 (CSN6), a subunit of COP9 signalosome, to prevent ubiquitination-mediated degradation of c-Myc protein in HSCs. As a consequence, forcing c-Myc expression improves the functional defect of Tespa1-null HSCs. On the other hand, Tespa1 is identified to be highly enriched in human acute myeloid leukemia (AML) cells and is essential for AML cell growth. Furthermore, using MLL-AF9-induced AML model, we find that Tespa1 deficiency suppresses leukemogenesis and LSC maintenance. In summary, our findings reveal the important role of Tespa1 in promoting HSC and LSC maintenance and therefore provide new insights on the feasibility of hematopoietic regeneration and AML treatment.
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- 2023
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5. Simulation investigation on marine exhaust gas SO2absorption by seawater scrubbing
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Li, Wenjun, Zhang, Yongxin, Zhao, Zhongyang, Liu, Chang, Wang, Yifan, Shen, Mingqiang, Dai, Haobo, Yang, Yang, Zheng, Chenghang, and Gao, Xiang
- Abstract
ABSTRACTShips have become an important source of SO2emission in coastal areas with the rapid development of maritime transport. It is of great significance to develop a marine scrubber for reducing SO2emission of ships. In this study, numerical simulation of a full-scale marine spray scrubber is conducted to investigate two-phase flow pattern and SO2absorption process in the scrubber. A desulfurization model based on seawater absorbent is coupled into the simulation, which considers the mass transfer between phases and seawater aqueous phase chemistry simultaneously. A distribution ring is introduced in the scrubber to enhance the desulfurization performance of the scrubber. The result of simulation shows that the distribution ring can optimize effectively the distribution of gas–liquid phases and enhance the SO2absorption. Under vertical condition, the desulfurization efficiency could be promoted approximate 6% after installing a distribution ring. The inclined condition resulting from the ship swinging could lead to the uneven distribution of droplets and an obvious decrease (8.7%) of desulfurization efficiency, whereas the desulfurization performance of the scrubber could be ensured with a distribution ring installed even under an inclined condition. Finally, a spray scrubber design scheme has been developed and successfully applied in the exhaust gas cleaning system (EGCS) of a container ship. Test result shows the outlet average value of SO2/CO2can be reduced to 3.55. Meanwhile, the consistency of test data and calculation result indicates the applicability of the numerical model established for the simulation and optimization of the scrubber in industrial applications also.Implications: EGCS is an effective method to reduce SO2emission of marine industry. However, different from a land desulfurization tower, the application of a spray scrubber in EGCS faces more problems due to the different application scenarios and complex sea conditions (inclined condition resulting from ships swinging and so on) during sailing. In this work, a numerical model capable of investigating physical and chemical phenomena in the scrubber simultaneously is established, which can produce a great amount of data for the operation instruction of EGCS and the design and optimization of the marine spray scrubber. The distribution ring is introduced in the marine spray scrubber to intensify the SO2absorption and enhance the desulfurization performance of the scrubber under different working conditions.
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- 2022
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6. CDK19 regulates the proliferation of hematopoietic stem cells and acute myeloid leukemia cells by suppressing p53-mediated transcription of p21
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Zhang, Zihao, Lu, Yukai, Qi, Yan, Xu, Yang, Wang, Song, Chen, Fang, Shen, Mingqiang, Chen, Mo, Chen, Naicheng, Yang, Lijing, Chen, Shilei, Wang, Fengchao, Su, Yongping, Hu, Mengjia, and Wang, Junping
- Abstract
The cell cycle progression of hematopoietic stem cells (HSCs) and acute myeloid leukemia (AML) cells is precisely controlled by multiple regulatory factors. However, the underlying mechanisms are not fully understood. Here, we find that cyclin-dependent kinase 19 (CDK19), not its paralogue CDK8, is relatively enriched in mouse HSCs, and its expression is more significantly increased than CDK8 after proliferative stresses. Furthermore, SenexinB (a CDK8/19 inhibitor) treatment impairs the proliferation and self-renewal ability of HSCs. Moreover, overexpression of CDK19 promotes HSC function better than CDK8 overexpression. Using CDK19 knockout mice, we observe that CDK19−/−HSCs exhibit similar phenotypes to those of cells treated with SenexinB. Interestingly, the p53 signaling pathway is significantly activated in HSCs lacking CDK19 expression. Further investigations show that CDK19 can interact with p53 to inhibit p53-mediated transcription of p21 in HSCs and treatment with a specific p53 inhibitor (PFTβ) partially rescues the defects of CDK19-null HSCs. Importantly, SenexinB treatment markedly inhibits the proliferation of AML cells. Collectively, our findings indicate that CDK19 is involved in regulating HSC and AML cell proliferation via the p53-p21 pathway, revealing a new mechanism underlying cell cycle regulation in normal and malignant hematopoietic cells.
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- 2022
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7. CD63 acts as a functional marker in maintaining hematopoietic stem cell quiescence through supporting TGFβ signaling in mice
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Hu, Mengjia, Lu, Yukai, Wang, Song, Zhang, Zihao, Qi, Yan, Chen, Naicheng, Shen, Mingqiang, Chen, Fang, Chen, Mo, Yang, Lijing, Chen, Shilei, Zeng, Dongfeng, Wang, Fengchao, Su, Yongping, Xu, Yang, and Wang, Junping
- Abstract
Hematopoietic stem cell (HSC) fate is tightly controlled by various regulators, whereas the underlying mechanism has not been fully uncovered due to the high heterogeneity of these populations. In this study, we identify tetraspanin CD63 as a novel functional marker of HSCs in mice. We show that CD63 is unevenly expressed on the cell surface in HSC populations. Importantly, HSCs with high CD63 expression (CD63hi) are more quiescent and have more robust self-renewal and myeloid differentiation abilities than those with negative/low CD63 expression (CD63-/lo). On the other hand, using CD63 knockout mice, we find that loss of CD63 leads to reduced HSC numbers in the bone marrow. In addition, CD63-deficient HSCs exhibit impaired quiescence and long-term repopulating capacity, accompanied by increased sensitivity to irradiation and 5-fluorouracil treatment. Further investigations demonstrate that CD63 is required to sustain TGFβ signaling activity through its interaction with TGFβ receptors I and II, thereby playing an important role in regulating the quiescence of HSCs. Collectively, our data not only reveal a previously unrecognized role of CD63 but also provide us with new insights into HSC heterogeneity.
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- 2021
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8. SRC-3 is involved in maintaining hematopoietic stem cell quiescence by regulation of mitochondrial metabolism in mice
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Hu, Mengjia, Zeng, Hao, Chen, Shilei, Xu, Yang, Wang, Song, Tang, Yong, Wang, Xinmiao, Du, Changhong, Shen, Mingqiang, Chen, Fang, Chen, Mo, Wang, Cheng, Gao, Jining, Wang, Fengchao, Su, Yongping, and Wang, Junping
- Abstract
Quiescence maintenance is an important property of hematopoietic stem cells (HSCs), whereas the regulatory factors and underlying mechanisms involved in HSC quiescence maintenance are not fully uncovered. Here, we show that steroid receptor coactivator 3 (SRC-3) is highly expressed in HSCs, and SRC-3–deficient HSCs are less quiescent and more proliferative, resulting in increased sensitivity to chemotherapy and irradiation. Moreover, the long-term reconstituting ability of HSCs is markedly impaired in the absence of SRC-3, and SRC-3 knockout (SRC-3−/−) mice exhibit a significant disruption of hematopoietic stem and progenitor cell homeostasis. Further investigations show that SRC-3 deficiency leads to enhanced mitochondrial metabolism, accompanied by overproduction of reactive oxygen species (ROS) in HSCs. Notably, the downstream target genes of peroxisome proliferator–activated receptor-coactivators 1α (PGC-1α) involved in the regulation of mitochondrial metabolism are significantly upregulated in SRC-3–deficient HSCs. Meanwhile, a significant decrease in the expression of histone acetyltransferase GCN5 accompanied by downregulation of PGC-1α acetylation is observed in SRC-3–null HSCs. Conversely, overexpression of GCN5 can inhibit SRC-3 deficiency-induced mitochondrial metabolism enhancement and ROS overproduction, thereby evidently rescuing the impairment of HSCs in SRC-3−/− mice. Collectively, our findings demonstrate that SRC-3 plays an important role in HSC quiescence maintenance by regulating mitochondrial metabolism.
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- 2018
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9. SRC-3 is involved in maintaining hematopoietic stem cell quiescence by regulation of mitochondrial metabolism in mice
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Hu, Mengjia, Zeng, Hao, Chen, Shilei, Xu, Yang, Wang, Song, Tang, Yong, Wang, Xinmiao, Du, Changhong, Shen, Mingqiang, Chen, Fang, Chen, Mo, Wang, Cheng, Gao, Jining, Wang, Fengchao, Su, Yongping, and Wang, Junping
- Abstract
Quiescence maintenance is an important property of hematopoietic stem cells (HSCs), whereas the regulatory factors and underlying mechanisms involved in HSC quiescence maintenance are not fully uncovered. Here, we show that steroid receptor coactivator 3 (SRC-3) is highly expressed in HSCs, and SRC-3–deficient HSCs are less quiescent and more proliferative, resulting in increased sensitivity to chemotherapy and irradiation. Moreover, the long-term reconstituting ability of HSCs is markedly impaired in the absence of SRC-3, and SRC-3 knockout (SRC-3−/−) mice exhibit a significant disruption of hematopoietic stem and progenitor cell homeostasis. Further investigations show that SRC-3 deficiency leads to enhanced mitochondrial metabolism, accompanied by overproduction of reactive oxygen species (ROS) in HSCs. Notably, the downstream target genes of peroxisome proliferator–activated receptor-coactivators 1α (PGC-1α) involved in the regulation of mitochondrial metabolism are significantly upregulated in SRC-3–deficient HSCs. Meanwhile, a significant decrease in the expression of histone acetyltransferase GCN5 accompanied by downregulation of PGC-1α acetylation is observed in SRC-3–null HSCs. Conversely, overexpression of GCN5 can inhibit SRC-3 deficiency-induced mitochondrial metabolism enhancement and ROS overproduction, thereby evidently rescuing the impairment of HSCs in SRC-3−/−mice. Collectively, our findings demonstrate that SRC-3 plays an important role in HSC quiescence maintenance by regulating mitochondrial metabolism.
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- 2018
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10. IGF-1 facilitates thrombopoiesis primarily through Akt activation
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Chen, Shilei, Hu, Mengjia, Shen, Mingqiang, Wang, Song, Wang, Cheng, Chen, Fang, Tang, Yong, Wang, Xinmiao, Zeng, Hao, Chen, Mo, Gao, Jining, Wang, Fengchao, Su, Yongping, Xu, Yang, and Wang, Junping
- Abstract
It is known that insulin-like growth factor-1 (IGF-1) also functions as a hematopoietic factor, although its direct effect on thrombopoiesis remains unclear. In this study, we show that IGF-1 is able to promote CD34+ cell differentiation toward megakaryocytes (MKs), as well as the facilitation of proplatelet formation (PPF) and platelet production from cultured MKs. The in vivo study demonstrates that IGF-1 administration accelerates platelet recovery in mice after 6.0 Gy of irradiation and in mice that received bone marrow transplantation following 10.0 Gy of lethal irradiation. Subsequent investigations reveal that extracellular signal-regulated kinase 1/2 (ERK1/2) and Akt activation mediate the effect of IGF-1 on thrombopoiesis. Notably, Akt activation induced by IGF-1 is more apparent than that of ERK1/2, compared with that of thrombopoietin (TPO) treatment. Moreover, the effect of IGF-1 on thrombopoiesis is independent of TPO signaling because IGF-1 treatment can also lead to a significant increase of platelet counts in homozygous TPO receptor mutant mice. Further analysis indicates that the activation of Akt triggered by IGF-1 requires the assistance of steroid receptor coactivator-3 (SRC-3). Therefore, our data reveal a distinct role of IGF-1 in regulating thrombopoiesis, providing new insights into TPO-independent regulation of platelet generation.
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- 2018
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11. IGF-1 facilitates thrombopoiesis primarily through Akt activation
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Chen, Shilei, Hu, Mengjia, Shen, Mingqiang, Wang, Song, Wang, Cheng, Chen, Fang, Tang, Yong, Wang, Xinmiao, Zeng, Hao, Chen, Mo, Gao, Jining, Wang, Fengchao, Su, Yongping, Xu, Yang, and Wang, Junping
- Abstract
It is known that insulin-like growth factor-1 (IGF-1) also functions as a hematopoietic factor, although its direct effect on thrombopoiesis remains unclear. In this study, we show that IGF-1 is able to promote CD34+cell differentiation toward megakaryocytes (MKs), as well as the facilitation of proplatelet formation (PPF) and platelet production from cultured MKs. The in vivo study demonstrates that IGF-1 administration accelerates platelet recovery in mice after 6.0 Gy of irradiation and in mice that received bone marrow transplantation following 10.0 Gy of lethal irradiation. Subsequent investigations reveal that extracellular signal-regulated kinase 1/2 (ERK1/2) and Akt activation mediate the effect of IGF-1 on thrombopoiesis. Notably, Akt activation induced by IGF-1 is more apparent than that of ERK1/2, compared with that of thrombopoietin (TPO) treatment. Moreover, the effect of IGF-1 on thrombopoiesis is independent of TPO signaling because IGF-1 treatment can also lead to a significant increase of platelet counts in homozygous TPO receptor mutant mice. Further analysis indicates that the activation of Akt triggered by IGF-1 requires the assistance of steroid receptor coactivator-3 (SRC-3). Therefore, our data reveal a distinct role of IGF-1 in regulating thrombopoiesis, providing new insights into TPO-independent regulation of platelet generation.
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- 2018
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12. Effect of radiation-induced endothelial cell injury on platelet regeneration by megakaryocytes.
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Chen, Fang, Shen, Mingqiang, Zeng, Dongfeng, Wang, Cheng, Wang, Song, Chen, Shilei, Tang, Yong, Hu, Mengjia, Chen, Mo, Su, Yongping, Ran, Xinze, Xu, Yang, and Wang, Junping
- Abstract
Thrombocytopenia is an important cause of hemorrhage and death after radiation injury, but the pathogenesis of radiation-induced thrombocytopenia has not been fully characterized. Here, we investigated the influence of radiation-induced endothelial cell injury on platelet regeneration. We found that human umbilical vein endothelial cells (HUVECs) underwent a high rate of apoptosis, accompanied by a significant reduction in the expression of vascular endothelial growth factor (VEGF) at 96 h after radiation. Subsequent investigations revealed that radiation injury lowered the ability of HUVECs to attract migrating megakaryocytes (MKs). Moreover, the adhesion of MKs to HUVECs was markedly reduced when HUVECs were exposed to radiation, accompanied by a decreased production of platelets by MKs. In vivo study showed that VEGF treatment significantly promoted the migration of MKs into the vascular niche and accelerated platelet recovery in irradiated mice. Our studies demonstrate that endothelial cell injury contributes to the slow recovery of platelets after radiation, which provides a deeper insight into the pathogenesis of thrombocytopenia induced by radiation.
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- 2017
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13. Indoxyl sulfate induces platelet hyperactivity and contributes to chronic kidney disease–associated thrombosis in mice
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Yang, Ke, Du, Changhong, Wang, Xinmiao, Li, Fengju, Xu, Yang, Wang, Song, Chen, Shilei, Chen, Fang, Shen, Mingqiang, Chen, Mo, Hu, Mengjia, He, Ting, Su, Yongping, Wang, Junping, and Zhao, Jinghong
- Abstract
Thrombosis is a common complication of chronic kidney disease (CKD), but the causes and mechanisms of CKD-associated thrombosis are not well clarified. Here, we show that platelet activity is remarkably enhanced in CKD mice, with increase of serum indoxyl sulfate (IS), a typical uremic toxin, which cannot be effectively cleared by routine dialysis. Ex vivo and in vitro experiments reveal that IS displays a distinct ability to enhance platelet activities, including elevated response to collagen and thrombin, increases in platelet-derived microparticles, and platelet-monocyte aggregates. The flow chamber assay and carotid artery thrombosis model demonstrate that IS-induced platelet hyperactivity contributes to thrombus formation. Further investigations disclose that reactive oxygen species (ROS)-mediated p38MAPK signaling plays a key role in IS-induced platelet hyperactivity. Moreover, we show that Klotho, which is expressed dominantly in the kidneys, has the capacity to counteract IS-induced platelet hyperactivity by inhibiting ROS/p38MAPK signaling, whereas Klotho reduction may aggravate the effect of IS on platelet activation in CKD and klotho+/−mice. Finally, we demonstrate that Klotho protein treatment can protect against IS-induced thrombosis and atherosclerosis in apoE−/−mice. Our findings uncover the mechanism of platelet hyperactivity induced by IS and provide new insights into the pathogenesis and treatment of CKD-associated thrombosis.
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- 2017
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14. Indoxyl sulfate induces platelet hyperactivity and contributes to chronic kidney disease–associated thrombosis in mice
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Yang, Ke, Du, Changhong, Wang, Xinmiao, Li, Fengju, Xu, Yang, Wang, Song, Chen, Shilei, Chen, Fang, Shen, Mingqiang, Chen, Mo, Hu, Mengjia, He, Ting, Su, Yongping, Wang, Junping, and Zhao, Jinghong
- Abstract
Thrombosis is a common complication of chronic kidney disease (CKD), but the causes and mechanisms of CKD-associated thrombosis are not well clarified. Here, we show that platelet activity is remarkably enhanced in CKD mice, with increase of serum indoxyl sulfate (IS), a typical uremic toxin, which cannot be effectively cleared by routine dialysis. Ex vivo and in vitro experiments reveal that IS displays a distinct ability to enhance platelet activities, including elevated response to collagen and thrombin, increases in platelet-derived microparticles, and platelet-monocyte aggregates. The flow chamber assay and carotid artery thrombosis model demonstrate that IS-induced platelet hyperactivity contributes to thrombus formation. Further investigations disclose that reactive oxygen species (ROS)-mediated p38MAPK signaling plays a key role in IS-induced platelet hyperactivity. Moreover, we show that Klotho, which is expressed dominantly in the kidneys, has the capacity to counteract IS-induced platelet hyperactivity by inhibiting ROS/p38MAPK signaling, whereas Klotho reduction may aggravate the effect of IS on platelet activation in CKD and klotho+/− mice. Finally, we demonstrate that Klotho protein treatment can protect against IS-induced thrombosis and atherosclerosis in apoE−/− mice. Our findings uncover the mechanism of platelet hyperactivity induced by IS and provide new insights into the pathogenesis and treatment of CKD-associated thrombosis.
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- 2017
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15. Sympathetic stimulation facilitates thrombopoiesis by promoting megakaryocyte adhesion, migration, and proplatelet formation
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Chen, Shilei, Du, Changhong, Shen, Mingqiang, Zhao, Gaomei, Xu, Yang, Yang, Ke, Wang, Xinmiao, Li, Fengju, Zeng, Dongfeng, Chen, Fang, Wang, Song, Chen, Mo, Wang, Cheng, He, Ting, Wang, Fengchao, Wang, Aiping, Cheng, Tianmin, Su, Yongping, Zhao, Jinghong, and Wang, Junping
- Abstract
The effect of sympathetic stimulation on thrombopoiesis is not well understood. Here, we demonstrate that both continual noise and exhaustive exercise elevate peripheral platelet levels in normal and splenectomized mice, but not in dopamine β-hydroxylase–deficient (Dbh−/−) mice that lack norepinephrine (NE) and epinephrine (EPI). Further investigation demonstrates that sympathetic stimulation via NE or EPI injection markedly promotes platelet recovery in mice with thrombocytopenia induced by 6.0 Gy of total-body irradiation and in mice that received bone marrow transplants after 10.0 Gy of lethal irradiation. Unfavorably, sympathetic stress-stimulated thrombopoiesis may also contribute to the pathogenesis of atherosclerosis by increasing both the amount and activity of platelets in apolipoprotein E–deficient (ApoE−/−) mice. In vitro studies reveal that both NE and EPI promote megakaryocyte adhesion, migration, and proplatelet formation (PPF) in addition to the expansion of CD34+ cells, thereby facilitating platelet production. It is found that α2-adrenoceptor–mediated extracellular signal-regulated kinase 1/2 (ERK1/2) activation is involved in NE- and EPI-induced megakaryocyte adhesion and migration, and PPF is regulated by ERK1/2 activation-mediated RhoA GTPase signaling. Our data deeply characterize the role of sympathetic stimulation in the regulation of thrombopoiesis and reevaluate its physiopathological implications.
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- 2016
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16. Sympathetic stimulation facilitates thrombopoiesis by promoting megakaryocyte adhesion, migration, and proplatelet formation
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Chen, Shilei, Du, Changhong, Shen, Mingqiang, Zhao, Gaomei, Xu, Yang, Yang, Ke, Wang, Xinmiao, Li, Fengju, Zeng, Dongfeng, Chen, Fang, Wang, Song, Chen, Mo, Wang, Cheng, He, Ting, Wang, Fengchao, Wang, Aiping, Cheng, Tianmin, Su, Yongping, Zhao, Jinghong, and Wang, Junping
- Abstract
The effect of sympathetic stimulation on thrombopoiesis is not well understood. Here, we demonstrate that both continual noise and exhaustive exercise elevate peripheral platelet levels in normal and splenectomized mice, but not in dopamine β-hydroxylase–deficient (Dbh−/−) mice that lack norepinephrine (NE) and epinephrine (EPI). Further investigation demonstrates that sympathetic stimulation via NE or EPI injection markedly promotes platelet recovery in mice with thrombocytopenia induced by 6.0 Gy of total-body irradiation and in mice that received bone marrow transplants after 10.0 Gy of lethal irradiation. Unfavorably, sympathetic stress-stimulated thrombopoiesis may also contribute to the pathogenesis of atherosclerosis by increasing both the amount and activity of platelets in apolipoprotein E–deficient (ApoE−/−) mice. In vitro studies reveal that both NE and EPI promote megakaryocyte adhesion, migration, and proplatelet formation (PPF) in addition to the expansion of CD34+cells, thereby facilitating platelet production. It is found that α2-adrenoceptor–mediated extracellular signal-regulated kinase 1/2 (ERK1/2) activation is involved in NE- and EPI-induced megakaryocyte adhesion and migration, and PPF is regulated by ERK1/2 activation-mediated RhoA GTPase signaling. Our data deeply characterize the role of sympathetic stimulation in the regulation of thrombopoiesis and reevaluate its physiopathological implications.
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- 2016
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17. hGH promotes megakaryocyte differentiation and exerts a complementary effect with c-Mpl ligands on thrombopoiesis
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Xu, Yang, Wang, Song, Shen, Mingqiang, Zhang, Zhou, Chen, Shilei, Chen, Fang, Chen, Mo, Zeng, Dongfeng, Wang, Aiping, Zhao, Jinghong, Cheng, Tianmin, Su, Yongping, and Wang, Junping
- Abstract
Human growth hormone (hGH) is known to play a functional role in regulating hematopoiesis, although its direct effect on thrombopoiesis is unclear. In this study, we show for the first time that hGH has a distinct capacity to promote the differentiation of human primary megakaryocytes derived from umbilical cord blood CD34+cells. In particular, hGH is potent in facilitating proplatelet formation and platelet production from cultured megakaryocytes. The stage- and time-specific activations of extracellular signal-regulated kinase 1/2 and protein kinase B signaling pathways are involved in the action of hGH. Fusion with hGH enhances the effect of a tandem dimer of thrombopoietin mimetic peptide (dTMP) on thrombopoiesis, manifested by a significant acceleration and increase of platelet production, indicating that hGH may exert a complementary and synergistic effect with c-Mpl ligands on thrombopoiesis. Accordingly, the administration of dTMP-growth hormone fusion protein led to a rapid platelet recovery in mice with severe thrombocytopenia induced by 6.5 Gy total body irradiation, thereby markedly abridging the duration of thrombocytopenia crisis (platelets <150 × 109/L), in comparison with high doses of dTMP. These findings demonstrate the functional role of growth hormone in promoting thrombopoiesis and provide a promising avenue for the treatment of severe thrombocytopenia.
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- 2014
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18. hGH promotes megakaryocyte differentiation and exerts a complementary effect with c-Mpl ligands on thrombopoiesis
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Xu, Yang, Wang, Song, Shen, Mingqiang, Zhang, Zhou, Chen, Shilei, Chen, Fang, Chen, Mo, Zeng, Dongfeng, Wang, Aiping, Zhao, Jinghong, Cheng, Tianmin, Su, Yongping, and Wang, Junping
- Abstract
Human growth hormone (hGH) is known to play a functional role in regulating hematopoiesis, although its direct effect on thrombopoiesis is unclear. In this study, we show for the first time that hGH has a distinct capacity to promote the differentiation of human primary megakaryocytes derived from umbilical cord blood CD34+ cells. In particular, hGH is potent in facilitating proplatelet formation and platelet production from cultured megakaryocytes. The stage- and time-specific activations of extracellular signal-regulated kinase 1/2 and protein kinase B signaling pathways are involved in the action of hGH. Fusion with hGH enhances the effect of a tandem dimer of thrombopoietin mimetic peptide (dTMP) on thrombopoiesis, manifested by a significant acceleration and increase of platelet production, indicating that hGH may exert a complementary and synergistic effect with c-Mpl ligands on thrombopoiesis. Accordingly, the administration of dTMP-growth hormone fusion protein led to a rapid platelet recovery in mice with severe thrombocytopenia induced by 6.5 Gy total body irradiation, thereby markedly abridging the duration of thrombocytopenia crisis (platelets <150 × 109/L), in comparison with high doses of dTMP. These findings demonstrate the functional role of growth hormone in promoting thrombopoiesis and provide a promising avenue for the treatment of severe thrombocytopenia.
- Published
- 2014
- Full Text
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19. Subcutaneous administration of rhIGF-I post irradiation exposure enhances hematopoietic recovery and survival in BALB/c mice
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Chen, Shilei, Xu, Yang, Wang, Song, Shen, Mingqiang, Chen, Fang, Chen, Mo, Wang, Aiping, Cheng, Tianmin, Su, Yongping, and Wang, Junping
- Abstract
It is unclear how to effectively mitigate against irradiation injury. In this study, we studied the capacity of recombinant human insulin-like growth factor-I (rhIGF-I) on hematologic recovery in irradiated BALB/c mice and its possible mechanism. BALB/c mice were injected with rhIGF-I subcutaneously at a dose of 100 µg/kg twice daily for 7 days after total body irradiation. Compared with a saline control group, treatment with rhIGF-I significantly improved the survival of mice after lethal irradiation (7.5 Gy). It was found that treatment with rhIGF-I not only could increase the frequency of Sca-1+ cells in bone marrow harvested at Day 14 after irradiation, but also it could decrease the apoptosis of mononuclear cells induced by irradiation as measured by flow cytometry, suggesting that rhIGF-I may mediate its effects primarily through promoting hematopoietic stem cell/progenitor survival and protecting mononuclear cells from apoptosis after irradiation exposure. Moreover, we have found that rhIGF-I might facilitate thrombopoiesis in an indirect way. Our data demonstrated that rhIGF-I could promote overall hematopoietic recovery after ionizing radiation and reduce the mortality when administered immediately post lethal irradiation exposure.
- Published
- 2012
- Full Text
- View/download PDF
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