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The evaluation of inoculation processes of microorganisms by robotic systems as well as by lab-technicians is compensable and can be missing consistency as human judgment will depend on the individual and may therefore be biased and less effective than models and algorithms evaluating spatial patterns. To address this problem, nearest neighbor analysis was used to investigate if it could be utilized as a method to evaluate isolation processes. The nearest neighbor analysis results in a comparable numeric value on the isolation process, which can be used to assess results of different inoculation processes.