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his paper describes the rules of change of reverse micelle phase separation under acid conditions. Using acetic acid, the reverse micelle system can be separated into an upper and lower phase. After separation with acid, the continuous phase is an organic solution and the polarity of upper phase is lower than that of lower layer. The separation formula is y=1.62+20.84e-x/5.31, where x is the W0 value of Superscript textthe original micelle system, y is the concentration of acetic acid required to separate the reverse micelle system into two phases of equal volume and it can be used to check the formula. Applying this formula to calculate the concentration of acid required to affect separation, the protein of rat serum can be separated under acidic conditions by the reverse micelle extraction method. In this way serum protein of rat can be further separated.

Hawthorn polyphenol (HP) was prepared by ethyl acetate treatment of the ethanol extract (HE) of Chinese hawthorn fruit. The concentrations of 15 polyphenols in the HP, HE, extraction residue (HJ), and a hawthorn leaf extract (HF) were determined by HPLC. For HP, the total content of the 15 polyphenols was 21.4%, comprised of 19.7% of procyanidins, 1.21% of chlorogenic acid, and 0.48% of flavonoids, compared to 2.55% for the HE. The yields of procyanidin monomer, dimer, trimer, tetramer, and pentamer were 50.5%, 30.3%, 23.0%, 14.6%, and 12.5% respectively, and the mean degree of polymerization was reduced to 1.39 (HP) from 1.65 (HE). Seven different physiological actions of the four extracts were investigated. The HP showed strong O2−and •OH scavenging capacities (IC50values of 6.3 μg/ml and 1.1 μg/ml respectively), as well as selective prolyl endopeptidase inhibition (IC50of 60 μg/ml). The active constituents appeared to be procyanidins.