1. Bacillus subtilisexhibits MnmC-like tRNA modification activities
- Author
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Moukadiri, Ismaïl, Villarroya, Magda, Benítez-Páez, Alfonso, and Armengod, M.-Eugenia
- Abstract
ABSTRACTThe MnmE-MnmG complex of Escherichia coliuses either ammonium or glycine as a substrate to incorporate the 5-aminomethyl or 5-carboxymethylaminomethyl group into the wobble uridine of certain tRNAs. Both modifications can be converted into a 5-methylaminomethyl group by the independent oxidoreductase and methyltransferase activities of MnmC, which respectively reside in the MnmC(o) and MnmC(m) domains of this bifunctional enzyme. MnmE and MnmG, but not MnmC, are evolutionarily conserved. Bacillus subtilislacks genes encoding MnmC(o) and/or MnmC(m) homologs. The glycine pathway has been considered predominant in this typical gram-positive species because only the 5-carboxymethylaminomethyl group has been detected in tRNALysUUUand bulk tRNA to date. Here, we show that the 5-methylaminomethyl modification is prevalent in B. subtilistRNAGlnUUGand tRNAGluUUC. Our data indicate that B. subtilishas evolved MnmC(o)- and MnmC(m)-like activities that reside in non MnmC homologous protein(s), which suggests that both activities provide some sort of biological advantage.
- Published
- 2018
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