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The selection process has caused modern durum wheat cultivars to achieve higher yields with different protein quality but also to have low micronutrient amounts. In order to evaluate the suitability of germplasm for the recovery of such nutrient content, macro- and microelements concentrations in twelve ancient Sicilian durum wheat landraces and in three modern cultivars were compared. According to the results, the substantial differences in macro- and micro-element concentrations between the two groups of wheat genotypes suggest ancient Sicilian landraces can effectively represent a suitable genetic material for biofortification plans of micronutrients in modern varieties.