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An efficient bio-conditioning of the nanomaterial for microbial inhibition and catalytic degradation has been recognized as a promising solution to protect public health and environmental safety. Herein, a bio-conditioned poly-dihydromyricetin-fused zinc nanoparticles (PDMY-Zn NPs) were designed and constructed based on a green tonic approach, which displays excellent processing efficiency against Escherichia coli(E. coli) and Staphylococcus aureus(S. aureus) as well as three dye effluents. Irregular-shaped PDMY-Zn NPs of size range 300–400 nm were formed by the undirected aggregation between Zn2+and dihydromyricetin (DMY) isolated from Ampelopsis grossedentata, contributing to the controlled release of Zn2+in gastrointestinal fluid. Such bio-conditioned nanomaterial relied on the stable and strong interaction between the bacterial membrane and carbohydrate polymeric DMY chain in PDMY-Zn NPs achieves a large amount of adhesion to both E. coliand S. aureus, which allows the targeted detachment of Zn2+from nanoparticles and accurate penetration into the pathogens, thereby achieving the nontoxicity to normal cells and minimizing drug-resistant bacteria. The division and spread of E. coliand S. aureustreated with PDMY-Zn NPs were effectively restricted and halted owing to multidimensional antibacterial behaviors of bacteriostatic agents, which are reflected in membrane damage, high cellular leakages and destroying intracellular ATP. Moreover, the designed PDMY-Zn NPs exhibit an efficient generation of hydroxyl (OH) and excess superoxide, which support it as a promising catalyst for photodegradation of methyl blue (93.02%), methyl orange (93.02%) and safranine (88.3%). Thus, this novel bio-conditioned nanomaterial provides a stable, nontoxic and advanced strategy to address drug-resistant bacteria infections as well as degradation of toxic dye effluents.

With the antibiotic crisis intensifies, the defense and treatment of pathogen infections in safe and effective fashion has become a critical issue. Herein, we report a novel and advanced type of sterilization agent designed via the functionalization DNA nanocarriers based on dihydromyricetin and CuO-loaded nanoparticles (DNA/DMY-CuO). Firstly, a pure dihydromyricetin (DMY) isolated from Ampelopsis grossedentatais used as a bridge to the stimulate the construction of DNA cross-linking networks by hydrogen bonding. Subsequently, a 3D spherical CuO-loaded nanocomposite (204.39 nm) is customized using the DNA/DMY network as a biological template through a simple coordination-assisted self-assembly method, which exhibits a high dispersibility, water-solubility and physiological stability. The reversible physical interactions in nanocarriers allows the selective separation and automatic release of CuO NPs from DNA/DMY-CuO in neutral and wound exudate environments, thereby extending the survival period of CuO NPs by nearly 24 h. Meanwhile, the nanocarriers system relied on the strong binding ability of DMY to the outer membrane of Escherichia coli(E. coli) and Staphylococcus aureus(S. aureus) achieves controlled drug delivery onto the pathogen wall. The advanced antibacterial action of DNA/DMY-CuO also reflected in membrane destruction, cytoplasmic constituent leakages and ATP synthetic pathway cessation, thereby halting cytosolic metalloregulatory mechanisms and minimizing drug-resistant bacteria. In summary, such multi-functional CuO-loaded nanocomposite provides a water-dispersibility, controllable, low cytotoxicity and long-effective platform to address the ever-growing threats of bacterial infections.