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We investigated promotion effects of exogenous sodium nitroprusside (SNP) on wheat seedling (Triticum aestivumL.) lateral root (LR) and root hair development, and the relationship between endogenous jasmonate (JA) production and activity changes of lipoxygenase (LOX) isoenzymes under osmotic stress generated by 15 % PEG-6000. Our results showed that 25 or 50 μM SNP could significantly increase LR length and number whether or not the seedlings were under PEG stress. When 50 μM cPTIO, 50 μM SHAM or 50 μM NDGA was supplemented, the promotion effects of SNP were blocked. SNP could also induce the production of endogenous JAs in roots, and 25 μM SNP induced the maximum JA content. The effect of SNP on JA production could also be blocked by adding cPTIO, SHAM or NDGA. Furthermore, the activity of lipoxygenase (LOX) in roots was affected by SNP; the maximal activity of LOX also occurred in the roots treated by 25 μM SNP under PEG stress, or 50 μM SNP without PEG stress. LOX isoenzymes in roots were detected by electrophoresis; the results showed that 25 μM SNP could noticeably increase the activities of LOXII and LOXIII under PEG stress. Our results suggest that, under osmotic stress generated by PEG, the promotion effects of exogenous SNP on wheat LR and root hair development could be mediated by endogenous JAs through LOX activation.We investigated promotion effects of exogenous sodium nitroprusside (SNP) on wheat seedling (Triticum aestivumL.) lateral root (LR) and root hair development, and the relationship between endogenous jasmonate (JA) production and activity changes of lipoxygenase (LOX) isoenzymes under osmotic stress generated by 15 % PEG-6000. Our results showed that 25 or 50 μM SNP could significantly increase LR length and number whether or not the seedlings were under PEG stress. When 50 μM cPTIO, 50 μM SHAM or 50 μM NDGA was supplemented, the promotion effects of SNP were blocked. SNP could also induce the production of endogenous JAs in roots, and 25 μM SNP induced the maximum JA content. The effect of SNP on JA production could also be blocked by adding cPTIO, SHAM or NDGA. Furthermore, the activity of lipoxygenase (LOX) in roots was affected by SNP; the maximal activity of LOX also occurred in the roots treated by 25 μM SNP under PEG stress, or 50 μM SNP without PEG stress. LOX isoenzymes in roots were detected by electrophoresis; the results showed that 25 μM SNP could noticeably increase the activities of LOXII and LOXIII under PEG stress. Our results suggest that, under osmotic stress generated by PEG, the promotion effects of exogenous SNP on wheat LR and root hair development could be mediated by endogenous JAs through LOX activation.

Salt marshes are ecologically and physically essential habitats in coastal intertidal flats. In addition to providing nursery grounds for various wildlife, vegetation in marshes acts as a buffer zone that slows flooding waters. Extensive marsh platforms can also attenuate wave energy to protect coastal communities. Complex geometries make it challenging for models to capture the spatiotemporal variabilities in the transport of lateral water and sediment over tidal creek‐marsh complexes. To study water exchange processes between tidal creeks and marshes, field observations were conducted within tidal creeks in the Chongming Dongtan Shoal of the Changjiang Estuary, an area dominated by Phragmites australis, Scirpus mariqueter, and Spartina alterniflora. A coupled wave‐current‐sediment‐vegetation model that parameterized the three vegetation species was developed and applied to the tidal creek complex of Chongming Dongtan. Validated with the observational data, the modeling results were used to examine the influence of vegetation on flooding/draining processes within tidal creeks and mudflats. The model showed that vegetation not only modified the flow pattern at high tide in the high‐elevation marsh but also significantly intensified the tidal flows in tidal creeks in the low‐elevation regions. Both the observations and model detected significant transport of water and sediment from the marsh to tidal creeks, seaward sediment export over tidal cycles. The simulated salinity exhibited a considerable spatial gradient, matching well with the salt tolerances of the major vegetation types. This model can be potentially used to determine how future scenarios of hydrodynamics could affect vegetation zonation. A vegetation model was developed to simulate the impacts of multiple vegetation species on flow, turbulence, waves and sedimentThree vegetation species were parameterized in the module, which was successfully applied to the tidal creek complex of Chongming DongtanModel demonstrated seaward sediment export from creeks, and consistency between the predicted salinity and the vegetation zonation A vegetation model was developed to simulate the impacts of multiple vegetation species on flow, turbulence, waves and sediment Three vegetation species were parameterized in the module, which was successfully applied to the tidal creek complex of Chongming Dongtan Model demonstrated seaward sediment export from creeks, and consistency between the predicted salinity and the vegetation zonation