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INTRODUCTION:The tumor cells could escape from the immune elimination through the immunoediting mechanisms including the generation of immunosuppressive or immunoregulative cells. By contrast, allograft transplantation could activate the immune system and induce a strong allogenic response. The aim of this study was to investigate the efficacy of allogenic skin transplantation in the inhibition of tumor growth through the activation of allogenic immune response. METHODS:Full-thickness skin transplantation was performed from C57BL/6 (H-2b) donors to BALB/c (H-2d) recipients that were receiving subcutaneous injection of isogenic CT26 colon cancer cells (2 × 106cells) at the same time. The tumor size and pathological changes, cell populations and cytokine profiles were evaluated at day 14 post-transplantation. RESULTS:The results showed that as compared to non-transplant group, the allogenic immune response in the skin-grafting group inhibited the growth of tumors, which was significantly associated with increased numbers of intra-tumor infiltrating lymphocytes, increased populations of CD11c+MHC-classII+CD86+DCs, CD3+CD4+T cells, CD3+CD8+T cells, and CD19+B cells, as well as decreased percentage of CD4+CD25+Foxp3+T cells in the spleens. In addition, the levels of serum IgM and IgG, tumor necrosis factor (TNF)-α and interferon (IFN)-γ were significantly higher within the tumor in skin transplant groups than that in non-transplant group. CONCLUSIONS:Allogenic skin transplantation suppresses the tumor growth through activating the allogenic immune response, and it may provide a new immunotherapy option for the clinical refractory tumor treatment.

Traditional methods for detecting lactoperoxidase (LP) are complex and time-consuming, so a test strip was made based on the enzymatic reaction principle to enable quick and convenient detection of LP in raw milk. In this study 0.1 mol/L citric acid (CA)/0.2 mol/L disodium hydrogen phosphate (NaP) buffer solution (pH 5.0), 22 mmol/L 3,3′,5,5′-tetramethylbenzidine (TMB), 0.6 mmol/L hydrogen peroxide (H2O2), and 0.5% Tween-20 or 0.3% cetyltrimethyl ammonium bromide (CTAB) were optimal for preparing a quick, sensitive, and accurate LP test strip. The coefficient of variation (CV) of the estimated LP concentrations ranged from 2.47% to 6.72% and the minimum LP concentration detected by the test strip was 1–2 mg/L. Estimates of active LP in sixteen raw milk samples obtained using the test strip or the TMB method showed a good correlation (r=0.9776). So the test strip provides a quick, convenient, and accurate method for detecting the LP concentration of raw milk.

Constraints and a driving were imposed to the three-dimensional model of slider-crank mechanism of the Mechatronics Fitness and Entertainment Machine modeled and assembled by UniGraphics 6.0 for dynamic simulation analysis in order to get velocity-time curve and acceleration-time curve. Usability of model was verified by comparing to the result of theoretical analysis of slider-crank mechanism. Finally, it was achieved to design the virtual prototype.

Wavefront correction plays significant role in some fields like astronomical observation, laser processing and medical imaging, etc. Liquid crystal spatial light modulator ( LC SLM) is an ideal device for high-resolution wavefront correction because of its low cost, low consumption, large number of pixels and independent programming control of each unit. It is researched experimentally that LC SLM is used as a wavefront correction device and corrects arbitrary wavefront aberration. Wavefront correction is performed based on phase conjugation and periodic phase modulation with modulo-2p. The experimental results show that the PV value of the irregular wavefront aberration is 1.56?, RMS value is 0.25 and Strehl ratio is 0.08 before correction, but the PV value of the residual aberration is reduced to 0.26?, RMS value is 0.02 and Strehl ratio is increased to 0.97 which is approximated diffraction limit after correction. It is proved to be feasible and effective that LC SLM is used to the high-precision and high-resolution wavefront correction.