31 results on '"Yang, Jinghua"'
Search Results
2. A New Approach to Clock Skewing for Area and Power Optimization of ASICs Using Differential Flipflops and Local Clocking
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Wagle, Ankit, Yang, Jinghua, Kulkarni, Niranjan, and Vrudhula, Sarma
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A new design methodology for reducing the area and power of standard cell ASICs that uses a combination of differential flipflops and a method of deliberate clock-skewing, called local clocking (LC), is described. LC introduces clock skew without the use of extra buffers in the clock network. This is done by having some flipflops, called sources, generate clock signals for other flipflops, called targets. The method involves two key features: 1) the design of a new differential flipflop, referred to as KVFF, that is functionally identical to a double-latch edge-triggered
$D$ - Published
- 2023
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3. Tomato leaf curl New Delhi virus: an emerging plant begomovirus threatening cucurbit production
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Cai, Lingmin, Mei, Yuzhen, Ye, Ruyi, Deng, Yun, Zhang, Xuejun, Hu, Zhongyuan, Zhou, Xueping, Zhang, Mingfang, and Yang, Jinghua
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Tomato leaf curl New Delhi virus (ToLCNDV), a bipartite begomovirus, was first reported to infect tomato and has recently spread rapidly as an emerging disease to Cucurbitaceaecrops. To date, the virus has been reported to infect more than 11 cucurbit crops, in 16 countries and regions, causing severe yield losses. In autumn 2022, ToLCNDV was first isolated from cucurbit plants in Southeastern coastal areas of China. Phylogenetic analysis established that these isolates belong to the Asian ToLCNDV clade, and shared high nucleotide identity and closest genetic relationship with the DNA-A sequence from the Chinese tomato-infecting ToLCNDV isolate (Accession no. OP356207) and the tomato New Delhi ToLCNDV-Severe isolate (Accession no. HM159454). In this review, we summarize the occurrence and distribution, host range, detection and diagnosis, control strategies, and genetic resistance of ToLCNDV in the Cucurbitaceae. We then summarize pathways that could be undertaken to improve our understanding of this emerging disease, with the objective to develop ToLCNDV-resistant cucurbit cultivars.
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- 2023
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4. Low-rank tensor approximation with local structure for multi-view intrinsic subspace clustering.
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Fu, Lele, Yang, Jinghua, Chen, Chuan, and Zhang, Chuanfu
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- 2022
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5. A study of the effect of dual shot peening on the surface integrity of carburized steel: combined experiments with dislocation density-based simulations
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Wu, Jizhan, Wei, Peitang, Guagliano, Mario, Yang, Jinghua, Hou, Shengwen, and Liu, Huaiju
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As a novel processing technology for high surface integrity, dual shot peening enables improvement in residual compressive stress, hardness gradient, and a reduction in surface roughness, thus effectively enhancing the fatigue resistance of mechanical components. The modelling and experiments on the dual shot peening of 18CrNiMo7-6 carburized gear steel have been conducted. A dual-shot peening finite element model based on the dislocation density constitutive equation that considers hardness gradient, residual stress, and surface roughness has been developed. Extensive peening tests and surface integrity characterisation using TEM and EBSD were conducted. The correlation between peening parameters and surface integrity and the underlying mechanism have been explored. The results from modelling and the experimental dual shot peening tests are in good agreement, with the maximum errors of residual stress and microhardness less than 15.1% and 7.4%, respectively. For an effective dual shot peening process, high-intensity peening with medium shots is recommended as the first step, and low-intensity peening as the second step. This process increases the surface residual compressive stress and hardness to -950 Mpa and 790 HV, respectively, while maintaining a reduced surface roughness.
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- 2024
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6. N/S co-doped CoSe/C nanocubes as anode materials for Li-ion batteries
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Cui, Lifeng, Qi, Haoyu, Wang, Nannan, Gao, Xin, Song, Chunyu, Yang, Jinghua, Wang, Gang, Kang, Shifeng, and Chen, Xiaodong
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The transition metal selenide can be used as a potential material for the negative electrode of lithium-ion batteries (LIBs) owing to its high density and conductivity. Unfortunately, a large volume change occurs in the transition metal selenide during the charging and discharging process, which eventually results in the poor rate performance and rapid capacity decay. In response to this, the N/S co-doped CoSe nanocubes (CoSe/C–NS) can be fabricated where the S-doped cobalt 2-methylimidazole (ZIF-67) as both sacrifice template and cobalt source to directly mix with selenium powder and followed by the annealing process. In the process, the carbon frameworks derived from ZIF-67 can establish a coating layer to protect the structure of materials, and simultaneously the N/S co-doping can enhance the conductivity and broaden the interlayer of frameworks. These can further accelerate the storage capacity and the Li+insertion and deintercalation process. As a negative electrode material of LIBs, the CoSe/C–NS delivers the high capacity, high rate performance, and long-term cycle stability. This protocol opens up an approvable approach to fabricate efficient anode materials with persistent electrochemical stability in LIBs.
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- 2022
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7. Genome structural evolution in Brassicacrops
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He, Zhesi, Ji, Ruiqin, Havlickova, Lenka, Wang, Lihong, Li, Yi, Lee, Huey Tyng, Song, Jiaming, Koh, Chushin, Yang, Jinghua, Zhang, Mingfang, Parkin, Isobel A. P., Wang, Xiaowu, Edwards, David, King, Graham J., Zou, Jun, Liu, Kede, Snowdon, Rod J., Banga, Surinder S., Machackova, Ivana, and Bancroft, Ian
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The cultivated Brassicaspecies include numerous vegetable and oil crops of global importance. Three genomes (designated A, B and C) share mesohexapolyploid ancestry and occur both singly and in each pairwise combination to define the Brassicaspecies. With organizational errors (such as misplaced genome segments) corrected, we showed that the fundamental structure of each of the genomes is the same, irrespective of the species in which it occurs. This enabled us to clarify genome evolutionary pathways, including updating the Ancestral Crucifer Karyotype (ACK) block organization and providing support for the Brassicamesohexaploidy having occurred via a two-step process. We then constructed genus-wide pan-genomes, drawing from genes present in any species in which the respective genome occurs, which enabled us to provide a global gene nomenclature system for the cultivated Brassicaspecies and develop a methodology to cost-effectively elucidate the genomic impacts of alien introgressions. Our advances not only underpin knowledge-based approaches to the more efficient breeding of Brassicacrops but also provide an exemplar for the study of other polyploids.
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- 2021
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8. A neural tensor decomposition model for high-order sparse data recovery.
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Liao, Tianchi, Yang, Jinghua, Chen, Chuan, and Zheng, Zibin
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DATA recovery , *CONVOLUTIONAL neural networks , *DEEP learning , *DECOMPOSITION method , *INPAINTING - Abstract
Tensor decomposition has attracted wide attention in the low-rank tensor completion (LRTC) problem because of its marvelous recovering ability to missing entries. However, previous LRTC methods are generally based on linear and shallow models, which are prone to overfitting when the data is sparse, resulting in significantly degraded performance. Meanwhile, the models are highly sensitive and suffer from the difficult rank selection problem. To address these issues, we propose an effective and novel tensor-ring (TR) decomposition method based on the convolutional computation (ConvTR), which can be regarded as a natural extension of deep learning models for the LRTC problem. Specifically, ConvTR employs a multi-layer convolutional neural network (CNN) to model the complex interactions between TR factors. Each element in the index vector of the observation tensor can be embedded as a corresponding tensor slice in the factor tensor decomposed by the TR model. These individual slice matrices are then concatenated to get a wider matrix used for extracting the nonlinear features by feeding them into a 2D convolutional layer. A fully-connected layer is utilized to aggregate the final convoluted features to a scalar value, which is the desired missing entry indexed by the original index vector exactly. Extensive experiments on various common datasets verified the effectiveness of the proposed method and demonstrated its superior to the traditional TR-based completion methods and other state-of-the-art network-based methods. [ABSTRACT FROM AUTHOR]
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- 2024
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9. Nitric oxide alleviates lead toxicity by inhibiting lead translocation and regulating root growth in watermelon seedlings
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Khan, Jehanzeb, Malangisha, Guy Kateta, Ali, Abid, Mahmoud, Ahmed, Yang, Jinghua, Zhang, Mingfang, and Hu, Zhongyuan
- Abstract
Lead (Pb) is one of the most abundant toxic heavy metals, which have a serious impact on the growth and yield of crop plants. Nitric oxide (NO) is a natural signaling molecule that regulates the growth and productivity of plants. Here, exogenous NO was found to enhance Pb tolerance in watermelon, which resulted in more Pb restriction in roots and less up-translocated Pb to aerial parts. Pb stress, however, led to an increase in shoot dry weight, root biomass, root relative water content, leaf malondialdehyde (MDA) content, and the total soluble protein content in leaves and roots. By contrast, shoot height and fresh weight, leaf biomass, and root MDA content were decreased under Pb stress. NO treatments alleviated Pb toxicity by decreasing Pb translocation, enhancing root growth (elongation and biomass), inducing antioxidant enzymes activities, and reducing root MDA contents in watermelon seedlings. In conclusion, our results provide useful insights into the mechanism of Pb tolerance in cucurbit crops and information for the cultivation management of watermelon in the presence of this heavy metal (Pb).
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- 2021
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10. Anticoagulation therapy patterns for acute treatment of venous thromboembolism in GARFIELD‐VTE patients
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Haas, Sylvia, Ageno, Walter, Weitz, Jeffrey I., Goldhaber, Samuel Z., Turpie, Alexander G.G., Goto, Shinya, Angchaisuksiri, Pantep, Dalsgaard Nielsen, Joern, Kayani, Gloria, Zaghdoun, Audrey, Farjat, Alfredo E., Schellong, Sebastian, Bounameaux, Henri, Mantovani, Lorenzo G., Prandoni, Paolo, Kakkar, Ajay K., Loualidi, Ab, Colak, Abdurrahim, Bezuidenhout, Abraham, Abdool‐Carrim, Abu, Azeddine, Addala, Beyers, Adriaan, Dees, Adriaan, Mohamed, Ahmed, Aksoy, Ahmet, Abiko, Akihiko, Watanabe, Akinori, Krichell, Alan, Fernandez, Alberto Alfredo, Tosetto, Alberto, Khotuntsov, Alexey, Oropallo, Alisha, Slocombe, Alison, Kelly, Allan, Clark, Amanda, Gad, Amr, Arouni, Amy, Schmidt, Andor, Berni, Andrea, Kleiban, Andres Javier, Machowski, Andrew, Kazakov, Andrey, Galvez, Angel, Lockman, Ann, Falanga, Anna, Chauhan, Anoop, Riera‐Mestre, Antoni, Mazzone, Antonino, D'Angelo, Armando, Herdy, Artur, Kato, Atsushi, Ebrahim, Ayman Abd Elhamid, Salem, Mahmoud, Husin, Azlan, Erdelyi, Barbara, Jacobson, Barry, Amann‐Vesti, Beatrice, Battaloglu, Bektas, Wilson, Benedicte, Cosmi, Benilde, Francois, Bergmann Jean, Toufek, Berremeli, Hunt, Beverley, Natha, Bhavesh, Mustafa, Bisher, Kho, Bonnie Chi Shan, Carine, Boulon, Zidel, Brian, Dominique, Brisot, Christophe, Brousse, Trimarco, Bruno, Luo, Canhua, Alberto Cuneo, Carlos, Sanchez Diaz, Carlos Jerjes, Schwencke, Carsten, Cader, Cas, Yavuz, Celal, Javier Zaidman, Cesar, Lunn, Charles, Wu, Chau‐Chung, Toh, Cheng Hock, Chiang, Chern‐En, Elisa, Chevrier, Hsia, Chien‐Hsun, Huang, Chien‐Lung, Kwok, Chi‐Hang Kevin, Wu, Chih‐Cheng, Huang, Chi‐Hung, Ward, Chris, Opitz, Christian, Jeanneret‐Gris, Christina, Ha, Chung Yin, Huang, Chun‐Yao, Bidi, Claude Luyeye, Smith, Clifford, Brauer, Cornelia, Lodigiani, Corrado, Francis, Couturaud, Wu, Cynthia, Staub, Daniel, Theodoro, Daniel, Poli, Daniela, Acevedo, Riesco, Adler, David, Jimenez, David, Keeling, David, Scott, David, Imberti, Davide, Creagh, Desmond, Helene, Desmurs‐Clavel, Hagemann, Dirk, Le Roux, Dirk, Skowasch, Dirk, Belenky, Dmitry, Dorokhov, Dmitry, Petrov, Dmitry, Zateyshchikov, Dmitry, Prisco, Domenico, Møller, Dorthe, Kucera, Dusan, Esheiba, Ehab M., Panchenko, Elizaveta, Dominique, Elkouri, Dogan, Emre, Kubat, Emre, Diaz Diaz, Enrique, Tse, Eric Wai Choi, Yeo, Erik, Hashas, Erman, Grochenig, Ernst, Tiraferri, Eros, Blessing, Erwin, Michèle, Escande Orthlieb, Usandizaga, Esther, Porreca, Ettore, Ferroni, Fabian, Nicolas, Falvo, Ayala‐Paredes, Félix, Koura, Firas, Henry, Fitjerald, Cosmi, Franco, Erdkamp, Frans, Kamalov, Gadel, Dalmau, Garcia‐Bragado, Damien, Garrigues, Klein, Garry, Shah, Gaurand, Hollanders, Geert, Merli, Geno, Plassmann, Georg, Platt, George, Poirier, Germain, Sokurenko, German, Haddad, Ghassan, Ali, Gholam, Agnelli, Giancarlo, Gan, Gin Gin, Kaye‐Eddie, Grace, Le Gal, Gregoire, Allen, Gregory, Llamas Esperón, Guillermo Antonio, Jean‐Paul, Guillot, Gerofke, Hagen, Elali, Hallah, Burianova, Hana, Ohler, Hans‐Juergen, Wang, Haofu, Darius, Harald, Gogia, Harinder S., Striekwold, Harry, Gibbs, Harry, Hasanoglu, Hatice, Turker, Hatice, Franow, Hendrik, De Raedt, Herbert, Schroe, Herman, ElDin, Hesham Salah, Zidan, Hesham, Nakamura, Hiroaki, Kim, Ho Young, Lawall, Holger, Zhu, Hong, Tian, Hongyan, Yhim, Ho‐Young, ten Cate, Hugo, Hwang, Hun Gyu, Shim, Hyeok, Kim, Igor, Libov, Igor, Sonkin, Igor, Suchkov, Igor, Song, Ik‐Chan, Kiris, Ilker, Staroverov, Ilya, Looi, Irene, De La Azuela Tenorio, Isabel M, Savas, Ismail, Gordeev, Ivan, Podpera, Ivo, Lee, Jae Hoon, Sathar, Jameela, Welker, James, Beyer‐Westendorf, Jan, Kvasnicka, Jan, Vanwelden, Jan, Kim, JangYong, Svobodova, Jaromira, Gujral, Jaspal, Marino, Javier, Galvar, Javier Tristan, Kassis, Jeannine, Kuo, Jen‐Yuan, Shih, Jhih‐Yuan, Kwon, JiHyun, Joh, Jin Hyun, Park, Jin Hyun, Kim, Jin Seok, Yang, Jinghua, Krupicka, Jiri, Lastuvka, Jiri, Pumprla, Jiri, Vesely, Jiri, Carlos Souto, Joan, Antônio Correa, João, Duchateau, Johan, Fletcher, John Perry, del Toro, Jorge, del Toro, Jorge, Chavez Paez, Jorge Guillermo, Araujo Filho, Jose Dalmo, Saraiva, Jose, Diaz Peromingo, Jose Antonio, Gomez Lara, Jose, Luis Fedele, Jose, Maria Surinach, Jose, Chacko, Joseph, Antonio Muntaner, Juan, Álvarez Benitez, Juan Carlos, Hoyos Abril, Juan Moreno, Humphrey, Julian, Bono, Julio, Kanda, Junji, Boondumrongsagoon, Juree, Yiu, Kai Hang, Chansung, Kanchana, Boomars, Karin, Burbury, Kate, Kondo, Katsuhiro, Karaarslan, Kemal, Takeuchi, Kensuke, Kroeger, Knut, Zrazhevskiy, Konstantin, Svatopluk, Koscál, Shyu, Kou‐Gi, Vandenbosch, Kristel, Chang, Kuan‐Cheng, Chiu, Kuan‐Ming, Jean‐Manuel, Kubina, Wern, Kwan Jing, Ueng, Kwo‐Chang, Norasetthada, Lalita, Binet, Laure, Chew, Lee Ping, Zhang, Lei, Cristina, Leone Maria, Tick, Lidwine, Beatriz Schiavi, Lilia, Wong, Lily Lee Lee, Borges, Lohana, Botha, Louis, Capiau, Luc, Timmermans, Luc, Eduardo López, Luciano, Ria, Luigi, Hernandez Blasco, Luis Manuel, Guzman, Luis Alberto, Flota Cervera, Luis, Isabelle, Mahe, Monreal Bosch, Manuel, de los Rios Ibarra, Manuel, Núñez Fernandez, Manuel, Carrier, Marc, Raul Barrionuevo, Marcelo, Alcocer Gamba, Marco Antonio, Cattaneo, Marco, Moia, Marco, Bowers, Margaret, Chetanachan, Mariam, Berli, Mario Alberto, Fixley, Mark, Faghih, Markus, Stuecker, Markus, Schul, Marlin, Banyai, Martin, Koretzky, Martin, Myriam, Martin, Gaffney, Mary Elizabeth, Hirano, Masao, Kanemoto, Masashi, Nakamura, Mashio, Tahar, Mersel, Emmanuel, Messas, Kovacs, Michael, Leahy, Michael, Levy, Michael, Munch, Michael, Olsen, Michael, De Pauw, Michel, Gustin, Michel, Van Betsbrugge, Michiel, Boyarkin, Mikhail, Homza, Miroslav, Koto, Modise, Abdool‐Gaffar, Mohamed, Nagib, Mohamed Ayman Fakhry, El‐Dessoki, Mohamed, Khan, Mohamed, Mohamed, Monniaty, Kim, Moo Hyun, Lee, Moon‐Hee, Soliman, Mosaad, Ahmed, Mostafa Shawky, el Bary, Mostafa Soliman Abd, Moustafa, Moustafa A., Hameed, Muhammad, Kanko, Muhip, Majumder, Mujibur, Zubareva, Nadezhda, Mumoli, Nicola, Abdullah, Nik Azim Nik, Makruasi, Nisa, Paruk, Nishen, Kanitsap, Nonglak, Duda, Norberto, Nordin, Nordiana, Nyvad, Ole, Barbarash, Olga, Gurbuz, Orcun, Vilamajo, Oscar Gomez, Nandayapa Flores, Oscar, Gur, Ozcan, Oto, Oztekin, Javier Marchena, Pablo, Carroll, Patrick, Lang, Pavel, MacCallum, Peter, von Bilderling, Peter Baron, Blombery, Peter, Verhamme, Peter, Jansky, Petr, Bernadette, Peuch, De Vleeschauwer, Philippe, Hainaut, Philippe, Ferrini, Piera Maria, Iamsai, Piriyaporn, Christian, Ponchaux, Viboonjuntra, Pongtep, Rojnuckarin, Ponlapat, Ho, Prahlad, Mutirangura, Pramook, Wells, Rachel, Martinez, Rafael, Miranda, Raimundo Tirado, Kroening, Ralf, Ratsela, Rapule, Reyes, Raquel Lopez, Diaz de Leon, Raul Franco, Wong, Raymond Siu Ming, Alikhan, Raz, Jerwan‐Keim, Reinhold, Otero, Remedios, Murena‐Schmidt, Renate, Canevascini, Reto, Ferkl, Richard, White, Richard, Van Herreweghe, Rika, Santoro, Rita, Klamroth, Robert, Mendes, Robert, Prosecky, Robert, Cappelli, Roberto, Spacek, Rudolf, Singh, Rupesh, Griffin, Sam, Na, Sang Hoon, Chunilal, Sanjeev, Middeldorp, Saskia, Nakazawa, Satoshi, Toh, See Guan, Christophe, Seinturier, Isbir, Selim, Raymundo, Selma, Ting, Seng Kiat, Motte, Serge, Aktogu, Serir Ozkan, Donders, Servaas, Cha, Seung Ick, Nam, Seung‐Hyun, Marie‐Antoinette, Sevestre‐Pietri, Maasdorp, Shaun, Sun, Shenghua, Wang, Shenming, Essameldin, Sherif Mohamed, Sholkamy, Sherif Mohamed, Kuki, Shintaro, Yoshida, Shuichi, Matsuoka, Shunzo, McRae, Simon, Watt, Simon, Patanasing, Siriwimon, Jean‐Léopold, Siwe‐Nana, Wongkhantee, Somchai, Bang, Soo‐Mee, Testa, Sophie, Zemek, Stanislav, Behrens, Steffen, Dominique, Stephan, Mellor, Stuart, Singh, Suaran Singh Gurcharan, Datta, Sudip, Chayangsu, Sunee, Solymoss, Susan, Everington, Tamara, Abdel‐Azim, Tarek Ahmed Adel, Suwanban, Tawatchai, Adademir, Taylan, Hart, Terence, Béatrice, Terriat, Luvhengo, Thifhelimbilu, Horacek, Thomas, Zeller, Thomas, Boussy, Tim, Reynolds, Tim, Biss, Tina, Chao, Ting‐Hsing, Casabella, Tomas Smith, Onodera, Tomoya, Numbenjapon, Tontanai, Gerdes, Victor, Cech, Vladimir, Krasavin, Vladimir, Tolstikhin, Vladimir, Bax, W.A., Malek, Wagih Fawzy Abdel, Ho, Wai Khoon, Pharr, Walter, Jiang, Weihong, Lin, Wei‐Hsiang, Zhang, Weihua, Tseng, Wei‐Kung, Lai, Wen‐Ter, De Backer, Wilfried, Haverkamp, Wilhelm, Yoshida, Winston, Korte, Wolfgang, Choi, Won II, Kim, Yang‐Ki, Tanabe, Yasuhiro, Ohnuma, Yasushi, Mun, Yeung‐Chul, Balthazar, Yohan, Park, Yong, Shibata, Yoshisato, Burov, Yuriy, Subbotin, Yuriy, Coufal, Zdenek, Yang, Zhenwen, Jing, Zhicheng, Jing, Zhicheng, and Yang, Zhongqi
- Abstract
Parenteral anticoagulants and vitamin K antagonists (VKAs) have constituted the cornerstone of venous thromboembolism (VTE) treatment. Meanwhile, direct oral anticoagulants (DOACs) provide physicians with an alternative. The Global Anticoagulant Registry in the FIELD (GARFIELD)‐VTE observes real‐world treatment practices.
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- 2019
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11. Biosynthetic pathways and related genes regulation of bioactive ingredients in mulberry leaves
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Lu, Na, Zhang, Lei, Tian, Yuqing, Yang, Jinghua, Zheng, Shicun, Wang, Liang, and Guo, Wei
- Abstract
ABSTRACTMulberry leaves are served not only as fodder for silkworms but also as potential functional food, exhibiting nutritional and medical benefits due to the complex and diverse constituents, including alkaloids, flavonoids, phenolic acids, and benzofurans, which possess a wide range of biological activities, such as anti-diabete, anti-oxidant, anti-inflammatory, and so on. Nevertheless, compared with the well-studied phytochemistry and pharmacology of mulberry leaves, the current understanding of the biosynthesis mechanisms and regulatory mechanisms of active ingredients in mulberry leaves remain unclear. Natural resources of these active ingredients are limited owing to their low contents in mulberry leaves tissues and the long growth cycle of mulberry. Biosynthesis is emerging as an alternative means for accumulation of the desired high-value compounds, which can broaden channels for their large-scale green productions. Therefore, this review summarizes the recent research advance on the correlative key genes, enzyme biocatalytic reactions and biosynthetic pathways of valuable natural ingredients (i.e. alkaloids, flavonoids, phenolic acids, and benzofurans) in mulberry leaves, thereby offering important insights for their further biomanufacturing.
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- 2023
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12. WciG O-Acetyltransferase Functionality Differentiates Pneumococcal Serotypes 35C and 42
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Geno, K. Aaron, Bush, C. Allen, Wang, Mengnan, Jin, Cheng, Nahm, Moon H., and Yang, Jinghua
- Abstract
ABSTRACTStreptococcus pneumoniaeexpresses capsular polysaccharides (CPSs) to protect itself from opsonophagocytic killing. The genes responsible for capsules synthesized by the Wzy-dependent mechanism, which accounts for 96 of the 98 known pneumococcal capsule types, are in a chromosomal region known as the cpslocus. The nucleotide sequence in this region has been determined for all serotypes. In contrast, not all CPS structures have been defined. The structure of the serotype 35C polysaccharide was recently reported, but the presence of O-acetyltransferase genes in the serotype 35C cpslocus suggested that it could be incomplete, as the reported structure contains no O-acetylation. In addition, the genetic distinction of serotype 35C from the closely related serotype 42 was unclear, as their reported cpsloci are nearly identical. To clarify these discrepancies, we obtained serotype 35C and 42 clinical and reference isolates and studied their serological and genetic properties, as well as the structures of CPSs purified from reference isolates. We demonstrated that the O-acetyltransferase WciG was functional in serotype 35C but nonfunctional in serotype 42 due to a deletion in wciG. Serotype 35C was O-acetylated at the 5- and 6-positions of 3-ß-galactofuranose, as well as the 2-position of 6-ß-galactofuranose. However, serotype 42 has only O-acetylation at 3-ß-galactofuranose, an observation consistent with its loss of WciG functionality, which is associated with O-acetylation at the 2-position and subsequent reaction with typing antiserum 35a. These findings provide a comprehensive view of the genetic, biochemical structural, and serological bases of serotypes 35C and 42.
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- 2017
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13. Insight into Enzymatic Degradation of Corn, Wheat, and Soybean Cell Wall Cellulose Using Quantitative Secretome Analysis of Aspergillus fumigatus
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Sharma Ghimire, Prakriti, Ouyang, Haomiao, Wang, Qian, Luo, Yuanming, Shi, Bo, Yang, Jinghua, Lü, Yang, and Jin, Cheng
- Abstract
Lignocelluloses contained in animal forage cannot be digested by pigs or poultry with 100% efficiency. On contrary, Aspergillus fumigatus, a saprophytic filamentous fungus, is known to harbor 263 glycoside hydrolase encoding genes, suggesting that A. fumigatusis an efficient lignocellulose degrader. Hence the present study uses corn, wheat, or soybean as a sole carbon source to culture A. fumigatusunder animal physiological condition to understand how cellulolytic enzymes work together to achieve an efficient degradation of lignocellulose. Our results showed that A. fumigatusproduced different sets of enzymes to degrade lignocelluloses derived from corn, wheat, or soybean cell wall. In addition, the cellulolytic enzymes produced by A. fumigatuswere stable under acidic condition or at higher temperatures. Using isobaric tags for a relative and absolute quantification (iTRAQ) approach, a total of ∼600 extracellular proteins were identified and quantified, in which ∼50 proteins were involved in lignocellulolysis, including cellulases, hemicellulases, lignin-degrading enzymes, and some hypothetical proteins. Data are available via ProteomeXchange with identifier PXD004670. On the basis of quantitative iTRAQ results, 14 genes were selected for further confirmation by RT-PCR. Taken together, our results indicated that the expression and regulation of lignocellulolytic proteins in the secretome of A. fumigatuswere dependent on both nature and complexity of cellulose, thus suggesting that a different enzyme system is required for degradation of different lignocelluloses derived from plant cells. Although A. fumigatusis a pathogenic fungus and cannot be directly used as an enzyme source, as an efficient lignocellulose degrader its strategy to synergistically degrade various lignocelluloses with different enzymes can be used to design enzyme combination for optimal digestion and absorption of corn, wheat, or soybean that are used as forage of pig and poultry.
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- 2016
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14. The genome sequence of allopolyploid Brassica juncea and analysis of differential homoeolog gene expression influencing selection
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Yang, Jinghua, Liu, Dongyuan, Wang, Xiaowu, Ji, Changmian, Cheng, Feng, Liu, Baoning, Hu, Zhongyuan, Chen, Sheng, Pental, Deepak, Ju, Youhui, Yao, Pu, Li, Xuming, Xie, Kun, Zhang, Jianhui, Wang, Jianlin, Liu, Fan, Ma, Weiwei, Shopan, Jannat, Zheng, Hongkun, Mackenzie, Sally A, and Zhang, Mingfang
- Abstract
The Brassica genus encompasses three diploid and three allopolyploid genomes, but a clear understanding of the evolution of agriculturally important traits via polyploidy is lacking. We assembled an allopolyploid Brassica juncea genome by shotgun and single-molecule reads integrated to genomic and genetic maps. We discovered that the A subgenomes of B. juncea and Brassica napus each had independent origins. Results suggested that A subgenomes of B. juncea were of monophyletic origin and evolved into vegetable-use and oil-use subvarieties. Homoeolog expression dominance occurs between subgenomes of allopolyploid B. juncea, in which differentially expressed genes display more selection potential than neutral genes. Homoeolog expression dominance in B. juncea has facilitated selection of glucosinolate and lipid metabolism genes in subvarieties used as vegetables and for oil production. These homoeolog expression dominance relationships among Brassicaceae genomes have contributed to selection response, predicting the directional effects of selection in a polyploid crop genome.
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- 2016
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15. One-pot hydrothermal synthesis of thrust spherical Mg–Al layered double hydroxides/MnO2and adsorption for Pb(II) from aqueous solutions
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Bo, Lifang, Li, Qiurong, Wang, Yihan, Gao, Lele, Hu, Xiaohui, and Yang, Jinghua
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- 2015
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16. Cell surface coaggregation receptor polysaccharide of oral streptococci
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Yoshida, Yasuo, Yang, Jinghua, Nagano, Keiji, Yoshimura, Fuminobu, and Cisar, John O.
- Abstract
Coaggregations of viridans group streptococci with other members of the oral microbial community play an important role in dental plaque biofilm formation. These interactions generally depend on lectin-like recognition of specific host-like motifs within the hexa- and heptasaccharide repeating units of different streptococcal receptor polysaccharides (RPS). To date, seven structural types of RPS (1Gn, 2Gn, 2G, 3Gn, 3G, 4Gn, and 5Gn) have been identified from over 25 different streptococcal strains. The repeating units of different RPS types invariably contain either GalNAcβ1-3Gal (Gn) or Galβ1-3GalNAc (G) disaccharide motifs, which are receptors of Actinomycesspp. type 2 fimbriae. Streptococci that bear Gn types of RPS also coaggregate with strains of Streptococcus gordoniiand S. sanguinisthat bear GalNAc-binding adhesins.
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- 2014
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17. Inaccuracy of transthoracic echocardiography for the identification of right‐sided vegetation in patients with no history of intravenous drug abuse or cardiac device insertion
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Xie, Jiang, Liu, Shuang, Yang, Jinghua, Xu, Jie, and Zhu, Guangfa
- Abstract
Objective: The use of transthoracic echocardiography (TTE) to identify right-sided infective endocarditis (RSIE) vegetation is controversial. Data are scarce for patients with no history of intravenous drug abuse (IVDA) or cardiac device insertion. This study analysed the consistency of presurgical echocardiographic results with surgical findings for vegetation identification, and the factors that influence accuracy of echocardiography.Methods: This retrospective trial divided infective endocarditis (IE) patients into three subgroups according to the results of their presurgical TTE: left-sided native IE (LSNIE), left-sided prosthetic valve IE (LSPIE) and RSIE. The accuracy of TTE was tested by comparing vegetation (number and location), detected presurgery by TTE, with actual findings during surgery.Results: In total, 416 patients were analysed, 322 with LSNIE, 31 with LSPIE and 63 with RSIE. Consistency between TTE findings and surgical results was lower in the RSIE group compared with the LSPIE and LSNIE groups. Consistency was lowered by the presence of vegetation in multiple locations and atypical distribution – both of which were increased in the RSIE group. The chance of vegetation in both sides of the heart rose with increased numbers of vegetation locations in RSIE patients. A high proportion of RSIE patients had congenital heart defects, mostly ventricular septal defects.Conclusions: TTE may be unsuitable for RSIE patients with no history of IVDA or cardiac device insertion, because multifocal and atypically distributed vegetation may influence detection accuracy.
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- 2014
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18. Roflumilast for the Treatment of COPD in an Asian Population
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Zheng, Jinping, Yang, Jinghua, Zhou, Xiangdong, Zhao, Li, Hui, Fuxin, Wang, Haoyan, Bai, Chunxue, Chen, Ping, Li, Huiping, Kang, Jian, Brose, Manja, Richard, Frank, Goehring, Udo-Michael, and Zhong, Nanshan
- Abstract
Roflumilast is the only oral phosphodiesterase 4 inhibitor indicated for use in the treatment of COPD. Previous studies of roflumilast have predominantly involved European and North American populations. A large study was necessary to determine the efficacy and safety of roflumilast in a predominantly ethnic Chinese population.
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- 2014
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19. The pointwise convergence of p-adic Möbius maps
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Wang, YueFei and Yang, JingHua
- Abstract
The convergence of linear fractional transformations is an important topic in mathematics. We study the pointwise convergence of p-adic Möbius maps, and classify the possibilities of limits of pointwise convergent sequences of Möbius maps acting on the projective line ℙ1(ℂp), where ℂpis the completion of the algebraic closure of ℚp. We show that if the set of pointwise convergence of a sequence of p-adic Möbius maps contains at least three points, the sequence of p-adic Möbius maps either converges to a p-adic Möbius map on the projective line ℙ1(ℂp), or converges to a constant on the set of pointwise convergence with one unique exceptional point. This result generalizes the result of Piranian and Thron (1957) to the non-archimedean settings.
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- 2014
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20. A natural mutation of the NST1gene arrests secondary cell wall biosynthesis in the seed coat of a hull-less pumpkin accession
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Lyu, Xiaolong, Shi, Lu, Zhao, Meng, Li, Zhangping, Liao, Nanqiao, Meng, Yiqing, Ma, Yuyuan, Zhou, Yulan, Xue, Qin, Hu, Zhongyuan, Yang, Jinghua, and Zhang, Mingfang
- Abstract
Hull-less pumpkins (Cucurbita pepoL.) are naturally occurring novel variants known as oilseed or naked-seeded pumpkins, and are characterized by the absence of a normal lignified seed coat. Due to a specialized seed coat structure, these variants serve as a good model for studying seed coat formation and simplify the processing of pumpkin seeds. However, causal genes for this hull-less trait still remain unknown. Here, by bulked segregant analysis and fine mapping, we found that mutation of a single gene, NAC SECONDARY WALL THICKENING PROMOTING FACTOR 1(NST1), accounts for the hull-less trait. A 14-bp sequence insertion in the CpNST1gene causes premature termination of CpNST1translation, leading to lack of secondary cell wall (SCW) biosynthesis in hull-less seed coats. In situhybridization analysis provided further evidence for the role of CpNST1in pumpkin seed coat SCW biosynthesis. Interestingly, through secondary cell wall compositional analysis, we found that the main SCW components differed among cell layers in the seed coat. RNA-seq analysis indicated an upstream role of CpNST1 in the SCW biosynthesis network. Collectively, our findings provide mechanistic insight into seed coat SCW biosynthesis, and a target gene for breeders to introduce this hull-less trait for commercial exploitation.
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- 2022
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21. Subgenome dominance and its evolutionary implications in crop domestication and breeding
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Wang, Zheng, Yang, Jinghua, Cheng, Feng, Li, Peirong, Xin, Xiaoyun, Wang, Weihong, Yu, Yangjun, Zhang, Deshuang, Zhao, Xiuyun, Yu, Shuancang, Zhang, Fenglan, Dong, Yang, and Su, Tongbing
- Abstract
Polyploidization or whole-genome duplication (WGD) is a well-known speciation and adaptation mechanism in angiosperms, while subgenome dominance is a crucial phenomenon in allopolyploids, established following polyploidization. The dominant subgenomes contribute more to genome evolution and homoeolog expression bias, both of which confer advantages for short-term phenotypic adaptation and long-term domestication. In this review, we firstly summarize the probable mechanistic basis for subgenome dominance, including the effects of genetic [transposon, genetic incompatibility, and homoeologous exchange (HE)], epigenetic (DNA methylation and histone modification), and developmental and environmental factors on this evolutionary process. We then move to Brassica rapa, a typical allopolyploid with subgenome dominance. Polyploidization provides the B. rapagenome not only with the genomic plasticity for adapting to changeable environments, but also an abundant genetic basis for morphological variation, making it a representative species for subgenome dominance studies. According to the ‘two-step theory’, B. rapaexperienced genome fractionation twice during WGD, in which most of the genes responding to the environmental cues and phytohormones were over-retained, enhancing subgenome dominance and consequent adaption. More than this, the pangenome of 18 B. rapaaccessions with different morphotypes recently constructed provides further evidence to reveal the impacts of polyploidization and subgenome dominance on intraspecific diversification in B. rapa. Above and beyond the fundamental understanding of WGD and subgenome dominance in B. rapaand other plants, however, it remains elusive why subgenome dominance has tissue- and spatiotemporal-specific features and could shuffle between homoeologous regions of different subgenomes by environments in allopolyploids. We lastly propose acceleration of the combined application of resynthesized allopolyploids, omics technology, and genome editing tools to deepen mechanistic investigations of subgenome dominance, both genetic and epigenetic, in a variety of species and environments. We believe that the implications of genomic and genetic basis of a variety of ecologically, evolutionarily, and agriculturally interesting traits coupled with subgenome dominance will be uncovered and aid in making new discoveries and crop breeding.
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- 2022
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22. Exploiting sterility and fertility variation in cytoplasmic male sterile vegetable crops
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Xu, Fengyuan, Yang, Xiaodong, Zhao, Na, Hu, Zhongyuan, Mackenzie, Sally A, Zhang, Mingfang, and Yang, Jinghua
- Abstract
Cytoplasmic male sterility (CMS) has long been used to economically produce hybrids that harness growth vigor through heterosis. Yet, how CMS systems operate within commercially viable seed production strategies in various economically important vegetable crops, and their underlying molecular mechanisms, are often overlooked details that could expand the utility of CMS as a cost-effective and stable system. We provide here an update on the nature of cytoplasmic–nuclear interplay for pollen sterility and fertility transitions in vegetable crops, based on the discovery of components of nuclear fertility restoration and reversion determinants. Within plant CMS systems, pollen fertility can be rescued by the introduction of nuclear fertility restorer genes (Rfs), which operate by varied mechanisms to countermand the sterility phenotype. By understanding these systems, it is now becoming feasible to achieve fertility restoration with Rfsdesigned for programmable CMS-associated open reading frames (ORFs). Likewise, new opportunities exist for targeted disruption of CMS-associated ORFsby mito-TALENs in crops where natural Rfshave not been readily identified, providing an alternative approach to recovering fertility of cytoplasmic male sterile lines in crops. Recent findings show that facultative gynodioecy, as a reproductive strategy, can coordinate the sterility and fertility transition in response to environmental cues and/or metabolic signals that reflect ecological conditions of reproductive isolation. This information is important to devising future systems that are more inherently stable.
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- 2022
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23. Study of Hemoglobin and Human Serum Albumin Glycation with Electrochemical Techniques
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Yang, Jinghua, Zhao, Jing, Xiao, Han, Zhang, Dongmei, and Li, Genxi
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High concentration glucose in diabetes mellitus may stimulate nonenzymatic glycation of proteins. Hemoglobin (Hb) and human serum albumin (HSA) are among the most sensitive proteins for the modification by glucose. In this paper, we report our study of Hb and HSA modification by glucose using electrochemical methods. Compared with native Hb, it is found that highly glycated Hb presents lower electron transfer reactivity and electrocatalytic activity toward O2and H2O2, and the glycation is glucose concentration and time dependent. Meanwhile, the changes of the electrochemical signal of heme after binding with HSA and glycated HSA have also suggested that proteins modified by high concentration glucose lasting for months and years in diabetes mellitus might be the reason for diabetes mellitus complication.
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- 2011
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24. Identification of new targets of Drosophilapre-mRNA adenosine deaminase
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Xia, Shuli, Yang, Jinghua, Su, Yingjun, Qian, Jiang, Ma, Enbo, and Haddad, Gabriel G.
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Adenosine deaminase acting on RNA (ADAR) in Drosophilaand mammals has recently become the target of numerous investigations. It is now clear that this protein has a number of functions in the nervous system. Indeed, the mutation of ADAR in Drosophila(dADAR) results in many pathological and physiological changes, such as sensitivity to hypoxia and neuronal degeneration. To understand the full scope of dADAR function, it is crucial to identify new dADAR targets. A polyclonal antibody against inosine was developed and used to enrich inosine-containing mRNAs. The efficiency of immunoaffinity purification was confirmed for the Q/R editing site of GluR-B pre-mRNA that has been edited by ADAR2 to generate inosines at the editing site. This approach was applied to enrich inosine-containing mRNAs from total mRNAs of wild-type and dADAR mutant flies, respectively. The enriched mRNA portion was then amplified and hybridized with DrosophilacDNA arrays. With this method, over 500 mRNAs were identified as potential dADAR targets by showing a higher amount in the enriched mRNA portion from wild-type flies than from dADAR mutant flies. The occurrence of A-to-G conversion in these mRNAs was further analyzed by comparing over 7,000 DrosophilacDNAs sequences with their genomic sequences. A final list of 62 candidates was generated from the overlap of the two approaches. Twelve genes from the final list were further examined by sequencing the RT-PCR products of these genes from wild-type and dADAR mutant flies. Seven of the 12 genes were proven to have A-to-G changes in the wild-type but not in mutant flies. We conclude that the combination of immunoaffinity enrichment of inosine-containing mRNA, DNA microarrays, and sequence comparison could facilitate the discovery of new dADAR substrates, which in turn allows us to better understand the targets of dADAR and the biological function of A-to-I RNA editing in flies.
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- 2005
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25. Human Glioma Cell BT325 Expresses a Proteinase That Converts Human Plasminogen to Kringle 1–5-Containing Fragments
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Li, Fuyang, Yang, Jinghua, Liu, Xinping, He, Peng, Ji, Shaoping, Wang, Jicun, Han, Jiong, Chen, Nanchun, and Yao, Libo
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Angiostatin, a specific angiogenesis inhibitor, is an internal fragment of plasminogen, and can be generated in many systems mediated by different enzymes in vitro. The mechanism of angiostatin generation in vivo has not been well defined. Here we demonstrated that human glioma cell line BT325 can express an enzyme that can convert purified plasminogen to angiostatin-like fragments with molecular masses of 65, 60, and 58 kDa, respectively. These fragments have an identical N-terminal as KVYLS, which starts from Lys98 of the plasminogen precusor. According to their molecular mass, the three fragments should comprise kringle domain 1 to kringle domain 5 (kringle 1–5). The proteolytic fragments obtained as above can inhibit the growth of bovine aortic endothelial (BAE) cells specifically. The proteolysis process can be completely inhibited by serine proteinase inhibitors, and partially inhibited by EDTA. The molecular weight of the peptide, which contains an enzymatic activity responsible for the proteolysis, was 13 kD determined by gel filtration and SDS–PAGE. The present data suggest that glioma cell BT325 can produce a novel proteinase to generate kringle 1–5 of plasminogen as an angiogenesis inhibitor.
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- 2000
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26. Reply to: ‘Organization of the genome sequence of the polyploid crop species Brassica juncea’
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Yang, Jinghua, Ji, Changmian, Liu, Dongyuan, Wang, Xiaowu, and Zhang, Mingfang
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- 2018
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27. Identification of eukaryotic translation initiation factors and the temperature-dependent nature of Turnip mosaic virusepidemics in allopolyploid Brassica juncea
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Shopan, Jannat, Liu, Chang, Hu, Zhongyuan, Zhang, Mingfang, and Yang, Jinghua
- Abstract
Eukaryotic translation initiation factors (eIFs) are essential protein complexes involved in the translation of mRNA into proteins. These initiation factors are generally used as targets in the control of plant RNA virus infections. In the present study, we identified a total 190 eIFs, clustered phylogenetically into 40 distinct subfamilies in the allopolyploid Brassica juncea. Extensive evolutionary duplications of the eIFs in B. junceasuggest their increased genetic diversity and wide adaptability. The induction of expressions in some of the eIFsafter inoculation against Turnip mosaic virus(TuMV) provided candidate targets to be used in the control of viral infections. In addition, the expression profiles of eIFsunder different temperatures suggested that the TuMV epidemic was temperature dependent. The eIFsexpressions suggested that the systemic viral infections were more acute in plants grown between 20 °C and 28 °C. In addition, our results revealed that new subgroups of eIFs, eIF2β, eIF2α, eIF2Bβ, EF1A, and PABPcould be represented as targets for antiviral strategies in B. juncea. In summary, our findings would be helpful in studying the complex mechanisms of eIF-mediated, temperature-dependent RNA virus control in B. juncea.
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- 2020
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28. Epidemic Situation of Brucellosis in Jinzhou City of China and Prediction Using the ARIMA Model
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Wang, Lulu, Liang, Chen, Wu, Wei, Wu, Shengwen, Yang, Jinghua, Lu, Xiaobo, Cai, Yuan, and Jin, Cuihong
- Abstract
Objective. This study aimed to investigate the specific epidemiological characteristics and epidemic situation of brucellosis in Jinzhou City of China so as to establish a suitable prediction model potentially applied as a decision-supportive tool for reasonably assigning health interventions and health delivery. Methods. Monthly morbidity data from 2004 to 2013 were selected to construct the autoregressive integrated moving average (ARIMA) model using SPSS 13.0 software. Moreover, stability analysis and sequence tranquilization, model recognition, parameter test, and model diagnostic were also carried out. Finally, the fitting and prediction accuracy of the ARIMA model were evaluated using the monthly morbidity data in 2014. Results. A total of 3078 cases affected by brucellosis were reported from January 1998 to December 2015 in Jinzhou City. The incidence of brucellosis had shown a fluctuating growth gradually. Moreover, the ARIMA(1,1,1)(0,1,1)12 model was finally selected among quite a few plausible ARIMA models based upon the parameter test, correlation analysis, and Box–Ljung test. Notably, the incidence from 2005 to 2014 forecasted using this ARIMA model fitted well with the actual incidence data. Notably, the actual morbidity in 2014 fell within the scope of 95% confidence limit of values predicted by the ARIMA(1,1,1)(0,1,1)12 model, with the absolute error between the predicted and the actual values in 2014 ranging from 0.02 to 0.74. Meanwhile, the MAPE was 19.83%. Conclusion. It is suitable to predict the incidence of brucellosis in Jinzhou City of China using the ARIMA(1,1,1)(0,1,1)12 model.
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- 2019
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29. Analysis and Reconstruction of Air-cooled Condenser Based on Proper Orthogonal Decomposition Technique
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Yang, Jinghua, and, Dengfeng Lu, and Cai, Li
- Abstract
Based on the previous experiments and numerical simulations, the low-order model of the air-cooled condenser was obtained by the orthogonal decomposition (POD) method, and the law of POD basis function and time coefficient distribution was obtained. Get the distribution of generalized "energy" between modes; The POD-based low-order model is used to reconstruct the pressure field and velocity field of the air-cooled condenser, and the relationship between the number of modes and the error of the result is obtained. The validity and rapidity of the reduced order method are proved by selecting the previous modal reconstruction flow fields.
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- 2018
30. Author Correction: The genome sequence of allopolyploid Brassica junceaand analysis of differential homoeolog gene expression influencing selection
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Yang, Jinghua, Liu, Dongyuan, Wang, Xiaowu, Ji, Changmian, Cheng, Feng, Liu, Baoning, Hu, Zhongyuan, Chen, Sheng, Pental, Deepak, Ju, Youhui, Yao, Pu, Li, Xuming, Xie, Kun, Zhang, Jianhui, Wang, Jianlin, Liu, Fan, Ma, Weiwei, Shopan, Jannat, Zheng, Hongkun, Mackenzie, Sally A, and Zhang, Mingfang
- Abstract
Following publication of this article, the authors have corrected 426 chimeric scaffolds in this genome (total scaffold number 10,684). The genome assembly has now been improved as V1.5, and the updated genome assembly is available to be downloaded from http://brassicadb.org/brad/datasets/pub/Genomes/Brassica_juncea/V1.5/.
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- 2018
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31. A compressed sensing-based progressive secret image sharing scheme and security analysis.
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Xie, Dong, Zhu, Huijun, Chen, Fulong, Wu, Bin, and Yang, Jinghua
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IMAGE compression , *SHIFT registers , *SHARING , *COMPRESSED sensing - Abstract
Cryptographic secret image sharing (SIS) allows a secret image to be shared among participants, and any authorized set of participants can reconstruct the secret. In this paper, we first propose a conceptually simple way to construct a progressive SIS scheme based on semi-tensor product compressed sensing (STP-CS). In the sharing phase, the dealer employs STP-CS to compress the secret image and extracts several different rows of the compressed image in turn. Then the sub-shadows for participants are formed. In addition, he needs to employ a perfect secret sharing to share the measurement matrix (or an initial state of the linear feedback shift register) among all participants. Finally, the sub-shadow and the share of the measurement matrix together constitute the shadow of each participant. In the combination phase, any legitimate access structure can reconstruct the secret image gradually. The perfect secret sharing ensures that the combination phase can work if and only if the access structure is authorized. Simultaneously, the use of STP-CS ensures that the proposed scheme has the properties of gradual reconstruction, noise-resilience, and smaller shadow size. Also, we have proven that the proposed scheme achieves the extended Wyner-sense perfect secrecy. Besides, we provide an extend version, referred to as homomorphic secret image sharing (HSIS), for improving the efficiency of the combination algorithm. HSIS can outsource the combination phase to the cloud servers securely. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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