1. Revisiting the role of delta-1-pyrroline-5-carboxylate synthetase in drought-tolerant crop breeding
- Author
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Feng, Xuanjun, Hu, Yue, Zhang, Weixiao, Xie, Rongqian, Guan, Huarui, Xiong, Hao, Jia, Li, Zhang, Xuemei, Zhou, Hanmei, Zheng, Dan, Wen, Ying, Wang, Qingjun, Wu, Fengkai, Xu, Jie, and Lu, Yanli
- Abstract
The delta-1-pyrroline-5-carboxylate synthetase (P5CS) gene exercises a protective function in stressed plants. However, the relationship between proline accumulation caused by P5CSand abiotic stress tolerance in plants is not always clear, as P5CSoverexpression has been reported to repress plant growth under normal conditions in several reports. We re-evaluated the role of P5CSin drought-tolerant rice breeding by expressing the AtP5CS1and feedback-inhibition-removed AtP5CS1(AtP5CS1F128A) genes under the regulation of an ABA-inducible promoter to avoid the potential side effects of P5CSoverexpression under normal conditions. ABA-inducible AtP5CS1and AtP5CS1F128Aincreased seedling growth in a nutrient solution (under osmotic stress) and grain yield in pot plants. However, the evidently deleterious effects of AtP5CS1on grain quality, tiller number, and grain yield in the field indicated the unsuitability of P5CSfor drought-tolerance breeding.
- Published
- 2022
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