1. Diversification and prevalence of the quinolone resistance crpPgenes and the crpP-carrying Tn6786-related integrative and conjugative elements in Pseudomonas aeruginosa
- Author
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Zhu, Zhichen, Yang, Huiying, Yin, Zhe, Jing, Ying, Zhao, Yuee, Fu, Hongyu, Du, Hong, and Zhou, Dongsheng
- Abstract
ABSTRACTThe quinolone resistance crpPgenes can mediate decreased susceptibility to quinolones. However, diversification and prevalence of crpPgenes and crpP-carrying integrative and conjugative elements (ICEs) still need to be elucidated. In this study, genome sequencing was conducted for 200 Chinese Pseudomonas aeruginosaisolates, 16 of which were fully sequenced. All the 37 available CrpP variants were collected for phylogenetic analysis, 10 CrpP enzymes were chosen to conduct cloning and antimicrobial susceptibility test, and 22 crpP-carrying Tn6786-related ICEs were selected for detail genetic dissection analysis. Then, typing/nomenclature schemes for crpPvariants and crpP-carrying ICEs were established for the first time. The 10 representative CrpP enzymes were confirmed to mediate decreased susceptibility to one to three quinolones. Tn6786-related ICEs displayed high-level diversification in both nucleotide sequences and modular structures. Mainly, massive gene acquisition/loss occurred across the whole genomes of Tn6786-related ICEs. 53.5% (107/200) of the tested clinical P. aeruginosaisolates from China carried crpPgenes, which were exclusively located within chromosome-borne Tn6786-related ICEs. The crpP-carrying ICEs were at active stages of evolution and had the high potential to be an important vector for the dissemination of resistance genes besides crpP. The present study furthered the understanding of the bioinformatics and epidemiology of crpPgenes and crpP-carrying ICEs.
- Published
- 2021
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