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Beam shaping of lasers is a topic that has received relatively less attention in the context of metal additive manufacturing (MAM) processes. This technique allows for modulation or spatial alternation of the intensity profile of the laser. As the bulk of the work within MAM primarily revolves around Gaussian beam profiles, the precise impact and potential of other beam shapes is still an unanswered question. In this work a multiphysics numerical model of the laser powder bed fusion (LPBF) process of Ti6Al4V without powder is developed and the model can predict thermo-fluid-microstructural conditions. The model predictions are compared with experimental data from single-track specimens, and the comparison shows a very good agreement. It is shown that the ring spot beam profile (RSBP) results in substantially wider melt pools as compared to the ones forming using the Gaussian beam profile (GBP). The microstructural predictions show that for GBP the grains converge to the center line of the melt pool, while for ring beam profile (RBP), the grains tend to converge to a single point. Finally, the impact of different ring radii for RBP is studied and the results show that at larger ring radii, a noticeable bulge of liquid metal forms right beneath the laser beam.

Laser beam shaping is a novel and relatively unexplored method for controlling the melt pool conditions during metal additive manufacturing (MAM) processes, but even so it still holds good promise for achieving site-specific tailored properties. In this work, a comprehensive numerical and experimental campaign is carried out to explore this subject within metal laser powder bed fusion (LPBF). More specifically, a multiphysics numerical model is implemented for simulating the heat and fluid flow conditions during LPBF of Ti6Al4V using arbitrary circular beam shapes with various power distributions spanning from a pure Gaussian beam to a pure ring beam profile. The model is subsequently coupled with cellular automata to describe the beam shape effects on the microstructure evolution. Model validation is carried out in a two-fold manner. First, we compare the predicted melt pool cross-section with the one from ex-situ single track experiments, and we find a deviation of less than 9 % in melt pool dimensions. Secondly, advanced in-situX-ray monitoring is carried out to unravel the melt pool dynamics and we find that the predicted morphology closely matches the in-situX-ray results. Moreover, it is shown that at lower laser power, a bulge of liquid metal forms at the center of the melt pool when employing ring profiles, and this is ascribed to the absence of recoil pressure at the center of the ring beam. Furthermore, increasing the laser power seems to destabilize the melt pool regime, as the central bulge transforms into a liquid metal jet that periodically collapses and breaks up into hot spatter. Based on the results, we believe that our multiphysics modelling methodology, opens up new pathways for predicting how laser beam shaping influences porosity, surface roughness as well as microstructure formation in LPBF processes.

Centromeric region of human chromosome 21 comprises two long alphoid DNA arrays: the well homogenized and CENP-B box-rich α21-I and the α21-II, containing a set of less homogenized and CENP-B box-poor subfamilies located closer to the short arm of the chromosome. Continuous alphoid fragment of 100 monomers bordering the non-satellite sequences in human chromosome 21 was mapped to the pericentromeric short arm region by fluorescence in situ hybridization (α21-II locus). The alphoid sequence contained several rearrangements including five large deletions within monomers and insertions of three truncated L1 elements. No binding sites for centromeric protein CENP-B were found. We analyzed sequences with alphoid/non-alphoid junctions selectively screened from current databases and revealed various rearrangements disrupting the regular tandem alphoid structure, namely, deletions, duplications, inversions, expansions of short oligonucleotide motifs and insertions of different dispersed elements. The detailed analysis of more than 1100 alphoid monomers from junction regions showed that the vast majority of structural alterations and joinings with non-alphoid DNAs occur in alpha satellite families lacking CENP-B boxes. Most analyzed events were found in sequences located toward the edges of the centromeric alphoid arrays. Different dispersed elements were inserted into alphoid DNA at kinkable dinucleotides (TG, CA or TA) situated between pyrimidine/purine tracks. DNA rearrangements resulting from different processes such as recombination and replication occur at kinkable DNA sites alike insertions but irrespectively of the occurrence of pyrimidine/purine tracks. It seems that kinkable dinucleotides TG, CA and TA are part of recognition signals for many proteins involved in recombination, replication, and insertional events. Alphoid DNA is a good model for studying these processes.

It can be invoked from the theory of tandem repeat homogenization that DNA on a satellite/non‐satellite border may carry sequence marks of molecular processes basic to satellite evolution. We have sequenced a continuous 17‐kb alpha satellite fragment bordering the non‐satellite in human chromosome 21, which is devoid of higher‐order repeated structure, contains multiple rearrangements, and exhibits higher divergence of monomers towards the border, indicating the lack of efficient homogenization. Remarkably, monomers have been found with mutually supplementary deletions matching each other as reciprocal products of unequal recombination, which provide evidence for unequal cross‐over as a mechanism generating deletions in satellite DNA.