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Chlorophyll, as a key component of crop leaves for photosynthesis, is one significant indicator for evaluating the photosynthetic efficiency and developmental status of crops. Fractional-order differentiation (FOD) enhances the feature spectral information and reduces the background noise. In this study, we analyzed hyperspectral data from grape leaves of different varieties and fertility periods with FOD to monitor the leaves' chlorophyll content (LCC). Firstly, through sensitive analysis, the fractional-order differential character bands were identified, which was used to construct the typical vegetation index (VI). Then, the grape LCC prediction model was built based on the random forest regression algorithm (RFR). The results showed the following: (1) FOD differential spectra had a higher sensitivity to LCC compared with the original spectra, and the constructed VIs had the best estimation performance at the 1.2th-order differential. (2) The accuracy of the FOD-RFR model was better than that of the conventional integer-order model at different fertility periods, but there were differences in the number of optimal orders. (3) The LCC prediction model for whole fertility periods achieved good prediction at order 1.3, R 2 = 0.778, RMSE = 2.1, and NRMSE = 4.7%. As compared to the original reflectance spectra, R2 improved by 0.173; RMSE and NRMSE decreased, respectively, by 0.699 and 1.5%. This indicates that the combination of FOD and RFR based on hyperspectral data has great potential for the efficient monitoring of grape LCC. It can provide technical support for the rapid quantitative estimation of grape LCC and methodological reference for other physiological and biochemical indicators in hyperspectral monitoring. [ABSTRACT FROM AUTHOR]

Objectives: The micro‐nano structure of 3D‐printed porous titanium (Ti) alloy with excellent performance in avoiding stress shielding and promoting bone tissue differentiation provides a new opportunity for the development of bone implants, but it necessitates higher requirements for bone tissue differentiation and the antibacterial properties of bone implants in clinical practice. Methods: This study investigated the preparation, antimicrobial properties, and osteogenesis‐promoting ability of the 3D printed porous Ti alloy anodic oxidized Ag‐carrying (Ag@3D‐TiO2) scaffolds. The 3D printed porous Ti alloy (3D‐Ti), anodized 3D printed porous Ti alloy (3D‐TiO2), and Ag@3D‐TiO2 scaffolds were synthesized using electron beam melting. The antimicrobial properties of the scaffolds were examined using antibacterial tests and their cytocompatibility was assessed using a cell proliferation assay and acridine orange/ethidium bromide (AO/EB) staining. In vitro cellular assays were used to investigate the effects of the scaffold microstructural features on cell activity, proliferation, and osteogenesis‐related genes and proteins. In vivo animal experiments were used to evaluate the anti‐inflammatory and osteogenesis‐promoting abilities of the scaffolds. Results: The Ag@3D‐TiO2 scaffolds exhibited sustained anti‐microbial activity over time, enhanced cell proliferation, facilitated osteogenic differentiation, and increased extracellular matrix mineralization. In addition, alkaline phosphatase (ALP), collagen type I (COL‐I), and osteocalcin (OCN)‐related genes and proteins were upregulated. In vivo animal implantation experiments, the anti‐inflammatory effect of the Ag@3D‐TiO2 scaffolds were observed using histology, and a large amount of fibrous connective tissue was present around it; the Ag@3D‐TiO2 scaffolds were more bio‐compatible with the surrounding tissues compared with 3D‐Ti and 3D‐TiO2; a large amount of uniformly distributed neoplastic bone tissue existed in their pores, and the chronic systemic toxicity test showed that the 3D‐Ti, 3D‐TiO2, and Ag@3D‐TiO2 scaffolds are biologically safe. Conclusion: The goal of this study was to create a scaffold that exhibits antimicrobial properties and can aid bone growth, making it highly suitable for use in bone tissue engineering. [ABSTRACT FROM AUTHOR]

To improve the quality of natural seawater to meet the needs of aquaculture production, a large-scale subsurface-flow constructed wetland (HSFCW) was constructed and operated stably for 2 years to understand the reasons for its purification effect on natural seawater. The results of the study showed that the system could maintain a high purification effect on natural seawater during aquaculture; the average removal rates of COD, TSS, TN, NH4+-N, and DON (organic nitrogen) were 22.29%, 49.33%, 36.94%, 10.88%, and 44.08%, respectively. Additionally, the HSFCW could effectively remove harmful algae such as Cyanobacteria; the removal rate of dominant algae species in Cyanobacteria was 90.33–97.93%. The pyrosequencing of 16S ribosomal DNA revealed that Proteobacteria, Nitrospirae, and Chloroflexi were the main and key bacterial phyla in the system. Members of these key gates are regarded as playing important roles in resisting water purification. The study results suggest that the subsurface-flow wetland system can effectively improve seawater quality and reduce the density of harmful algae cells. [ABSTRACT FROM AUTHOR]

Polyvinylidene fluoride‐hexafluoropropylene (PVDF‐HFP) is a standard electrolyte membrane and binder material for lithium‐ion batteries (LIBs) because of the benefits of membrane formation and strong mechanical properties. Still, its use is limited due to unavoidable defects such as high crystallinity and poor electrochemical performance. In this paper, a larger specific surface area and three‐dimensional (3D) permeable lamellae carboxylated g‐C3N4 (HCN) were successfully prepared by HNO3 and introduced into the PVDF‐HFP to obtain a high‐performance composite separator. The wettability and electrolyte absorption of PVDF‐HFP separator can be improved through the carboxylated HCN. More crucially, because PVDF‐HFP has several active sites and low crystallinity, adding HCN can greatly increase the ionic conductivity of separators. The ionic conductivity of PH‐HCN at 25 °C is 0.52 S cm−1, 4.7 times higher than the initial PVDF‐HFP membrane, and the oxidation potential can reach 4.8 V. The assembled lithium‐ion batteries half‐cell has a capacity of 71 % at a 5 C rate and 94 % after 400 cycles at a 2 C rate. Therefore, the improved separator has the potential to be applied to high‐performance LIBs. [ABSTRACT FROM AUTHOR]

Objectives: The micro-nano structure of 3D-printed porous titanium (Ti) alloy with excellent performance in avoiding stress shielding and promoting bone tissue differentiation provides a new opportunity for the development of bone implants, but it necessitates higher requirements for bone tissue differentiation and the antibacterial properties of bone implants in clinical practice., Methods: This study investigated the preparation, antimicrobial properties, and osteogenesis-promoting ability of the 3D printed porous Ti alloy anodic oxidized Ag-carrying (Ag@3D-TiO 2 ) scaffolds. The 3D printed porous Ti alloy (3D-Ti), anodized 3D printed porous Ti alloy (3D-TiO 2 ), and Ag@3D-TiO 2 scaffolds were synthesized using electron beam melting. The antimicrobial properties of the scaffolds were examined using antibacterial tests and their cytocompatibility was assessed using a cell proliferation assay and acridine orange/ethidium bromide (AO/EB) staining. In vitro cellular assays were used to investigate the effects of the scaffold microstructural features on cell activity, proliferation, and osteogenesis-related genes and proteins. In vivo animal experiments were used to evaluate the anti-inflammatory and osteogenesis-promoting abilities of the scaffolds., Results: The Ag@3D-TiO 2 scaffolds exhibited sustained anti-microbial activity over time, enhanced cell proliferation, facilitated osteogenic differentiation, and increased extracellular matrix mineralization. In addition, alkaline phosphatase (ALP), collagen type I (COL-I), and osteocalcin (OCN)-related genes and proteins were upregulated. In vivo animal implantation experiments, the anti-inflammatory effect of the Ag@3D-TiO 2 scaffolds were observed using histology, and a large amount of fibrous connective tissue was present around it; the Ag@3D-TiO 2 scaffolds were more bio-compatible with the surrounding tissues compared with 3D-Ti and 3D-TiO 2 ; a large amount of uniformly distributed neoplastic bone tissue existed in their pores, and the chronic systemic toxicity test showed that the 3D-Ti, 3D-TiO 2 , and Ag@3D-TiO 2 scaffolds are biologically safe., Conclusion: The goal of this study was to create a scaffold that exhibits antimicrobial properties and can aid bone growth, making it highly suitable for use in bone tissue engineering., (© 2024 The Authors. Orthopaedic Surgery published by Tianjin Hospital and John Wiley & Sons Australia, Ltd.)

Objective: This study aimed to examine the correlation between selenium intake and lung function in asthmatic people., Methods: A total of 4,541 individuals in the US National Health and Nutrition Examination Survey (NHANES) were included in this study. Multivariate linear regression, variance inflation factor, restricted cubic splines and quantile regression were used to analyze the relationship between Se intake and lung function. We divided selenium intake into four levels based on quartiles: Q1: Se ≤ 76.75 mcg/d; Q2: 76.75-105.1 mcg/d; Q3: 105.1-137.65 mcg/d; and Q4: Se ≥137.65 mcg/d., Results: Asthma was negatively associated with the Ratio of Forced Expiratory Volume 1st Second to Forced Vital Capacity (FEV1/FVC) (β = -0.04, 95% CI: -0.06 to -0.02) and FEV1 (β = -215, 95% CI: -340 to -90). Se intake was positively associated with Forced Expiratory Volume 1st Second (FEV1) (β =3.30 95% CI: 2.60 to 4.00) and Forced Vital Capacity (FVC) (β =4.30, 95% CI: 3.50 to 5.10). In asthmatic individuals, the positive effects of Se intake on FVC were enhanced with increasing Se intake, while the positive effects of Se intake on FEV1 varied less dramatically. High Se intake (Q4 level, above 137.65 mcg/d) improved FVC (β = 353, 95% CI: 80 to 626) and FEV1 (β = 543, 95% CI: 118 to 969) in asthmatic patients compared to low Se intake (Q1 level, below 76.75 mcg/d). At the Q2 level (76.75-105.1 mcg/d) and Q4 level (Se ≥137.65 mcg/d) of Se intake, the correlation between FEV1 and asthma disappeared., Conclusion: Our research has revealed a positive correlation between selenium intake and lung function in asthma patients and the strength of this positive correlation is related to the amount of selenium intake. We recommend that asthma patients consume 137.65 mcg to 200 mcg of selenium daily to improve pulmonary function while avoiding the adverse effects of selenium on the human body., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2024 Jiang, Yang, Chen, Yuan, Wu, Zhang, Zhang, Yuan, Lin, Chen and Yin.)