1. Circulating Mirna Spaceflight Signature Reveals Targets to Mitigate Associated Health Risks
- Author
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Sherina Malkani, Christopher R. Chin, Egle Cekanaviciute, Marie Mortreux, Hazeem Okinula, Marcel Tarbier, Ann-sofie Schreurs, Yasaman Shirazi-fard, Candice Tahimic, Deyra N. Rodriguez, Brittany S. Sexton, Daniel Butler, Akanksha Verma, Daniela Bezdan, Ceyda Durmaz, Matthew MacKay, Ari Melnick, Cem Meydan, Sheng Li, Francine Garrett-Bakelman, Bastian Fromm, Brad W. Langhorst, Eileen T. Dimalanta, Margareth Cheng-Campbell, Elizabeth Blaber, Charles Vanderburg, Marc R. Friedländer, J. Tyson McDonald, Sylvain V Costes, Seward Rutkove, Peter Grabham, Christopher E. Mason, and Afshin Beheshti
- Subjects
Aerospace Medicine - Abstract
We have identified and validated a spaceflight-associated microRNA (miRNA) signature that is shared by rodents and humans in response to simulated, short-duration, and long-duration spaceflight and regulates vascular damage caused by simulated deep space radiation. In previous studies, we had identified miRNAs that are predicted to regulate rodent responses to spaceflight in low-Earth orbit. Here we have confirmed the expression of these proposed spaceflight associated miRNAs in rodents reacting to simulated spaceflight conditions (exposure to ionizing radiation combined with simulated microgravity) and in astronaut samples from the NASA Twins Study via direct quantification of miRNAs, miRNA sequencing, and inferring miRNA target levels based on single-cell RNA (scRNA-seq) and single-cell chromatin (scATAC-seq) sequencing data. To demonstrate the physiological relevance of key spaceflight associated miRNAs, we utilized antagomirs to inhibit their expression and successfully rescue simulated deep space radiation-mediated damage in human 3D vascular constructs.
- Published
- 2020