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Previously, a synchrotron-based horizontal proton beamline (87.2 MeV) was successfully commissioned to deliver radiation doses in FLASH and conventional dose rate modes to small fields and volumes. In this study, we developed a strategy to increase the effective radiation field size using a custom robotic motion platform to automatically shift the positions of biological samples. The beam was first broadened with a thin tungsten scatterer and shaped by customized brass collimators for irradiating cell/organoid cultures in 96-well plates (a 7-mm-diameter circle) or for irradiating mice (1-cm2 square). Motion patterns of the robotic platform were written in G-code, with 9-mm spot spacing used for the 96-well plates and 10.6-mm spacing for the mice. The accuracy of target positioning was verified with a self-leveling laser system. The dose delivered in the experimental conditions was validated with EBT-XD film attached to the 96-well plate or the back of the mouse. Our film-measured dose profiles matched Monte Carlo calculations well (1D gamma pass rate >95%). The FLASH dose rates were 113.7 Gy/s for cell/organoid irradiation and 191.3 Gy/s for mouse irradiation. These promising results indicate that this robotic platform can be used to effectively increase the field size for preclinical experiments with proton FLASH.

Methods: A phase space file in a plane at 202 mm downstream of the beam exit window is generated through tuning parameters to match FDC results with measured or MCNPX Monte Carlo-simulated integrated depth-dose distribution (IDD) and lateral dose profiles. To spread out the Bragg peak, widen the beam and reduce the penumbra, a ridge filter (RF), a high-Z material scatterer and a collimator with compensator are inserted in the beam path and their shapes and sizes have been optimized. The FDC calculations are validated by comparing Geant4 Monte Carlo simulations. In addition, a set of algorithms to automatically choose the optimum dimensions of the beam shaping elements is developed and tested using the same beams. At the last part, dose rates for optimized beams were estimated by scaling their dose distributions to that of their original beams. Results: The optimized 86.4 MeV beam had an 8.5 mm wide spread-out Bragg peak (SOBP) (proximal 90% to distal 90% of the maximum dose), 14.5 mm, 12.0 mm and 11.0 lateral widths with dose above 50%, 80% and 90% respectively and a 2.5 mm penumbra from 80% to 20% in the lateral profile for the energy. The 159.5 MeV beam had a SOBP of 39.0 mm and the lateral widths with dose above 50%, 80% and 90% of 20.5 mm, 15.0 and 12.5 mm when the source to surface distance (SSD) was 550 mm. Wider lateral widths was obtained with increased SSD. The FDC calculations had passing rates higher than 96% using 3mm/3% as the gamma-index criterion comparing with Geant4 simulations for both energies. The set of automatic algorithms can choose the proper dimensions for the high-density scatterer, RF, collimator and compensator efficiently. And the optimized 159.5 MeV beam with different SDDs had entrance dose rate higher than 40 Gy/s if the entrance dose rate of the original beam was 150 Gy/s., Comment: 15 pages, 7 figures, two tables