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We designed and developed a set of free-response questions to assess learning assistants pedagogical content knowledge in the context of questioning (PCK-Q). Our goal was to develop a set of written free-response questions that would be representative of the LAs PCK-Q observed in the classroom. In this paper we discuss the design, development and validation processes. These include videotaping and analysis of classroom interactions, development of a coding rubric, and development, administration, and validation of written free-response questions.