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Studies of meteorite analysis and theoretical modeling have indicated the possibility that some carbonaceous near-Earth asteroids are thermally altered due to radiative heating during close approaches to the Sun in addition to parent body processes (Nakamura, 2005; Marchi et al., 2009; Chaumard et al., 2012). In April 2019, the Hayabusa2 mission successfully completed an artificial impact experiment on the carbonaceous near-Earth asteroid 162173 Ryugu (Arakawa et al., 2020), which provided an opportunity to investigate the effects of radiative heating through the exposed subsurface material. Here we report observations of the Ryugu’s subsurface material by the Near-Infrared Spectrometer (NIRS3) on the Hayabusa2 spacecraft. Spectra of the subsurface material exhibit a slightly stronger and peak-shifted hydroxyl absorption feature compared to that observed for the surface, indicating that space weathering and/or radiative heating caused a subtle change in the spectrum of Ryugu surface. However, the shape of the absorption feature still suggests that the subsurface material experienced heating above 300 ˚C similar to the surface. In contrast, our thermal modeling shows that radiative heating does not increase the subsurface temperature at 1 m depth above 200 ˚C even if the semimajor axis is reduced down to 0.344 au. This supports that the Ryugu material would have been preferentially altered due to radiogenic and/or impact heating on the parent body rather than radiative heating.